Cellular and molecular basis for the action of retinoic acid in spermatogenesis

Griswold, Michael D.

doi:10.1530/jme-22-0067

Cited by 10 publications

(8 citation statements)

References 58 publications

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“…Several Sertoli cell expressed proteins, such as the type III intermediate filament proteins vimentin, desmin and peripherin (Mruk and Cheng, 2004, Vogl et al, 2008) as well as fatty acid-binding protein 5 (FABP5) and intracellular retinol-binding protein 1 (RBP1) (Oresti et al, 2013, Griswold, 2022), were found to be higher abundant in Actl7b-/- testicular samples. Differential abundance of these mostly Sertoli cell enriched proteins might indicate a secondary effect caused by arrested and apoptotic germ cells.…”

Section: Resultsmentioning

confidence: 99%

Actl7b-deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis

Merges

Arévalo

Lohanadan

et al. 2022

Preprint

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Actin-related proteins (Arp) are classified according to their similarity to actin and are involved in diverse cellular processes. ACTL7B is a testis-specific Arp and highly conserved in rodents and primates. ACTL7B is specifically expressed in round and elongating spermatids during spermiogenesis. Here, we have generated an Actl7b-null allele in mice to unravel the role of ACTL7B in sperm formation. Male mice homozygous for the Actl7b-null allele (Actl7b-/-) were infertile, while heterozygous males (Actl7b+/-) were fertile. Severe spermatid defects such as detached acrosomes, disrupted membranes and failed elongation of the axoneme start to accumulate at step 9 in Actl7b-/- mice, finally resulting in spermatogenic arrest. Abnormal spermatids, became degraded. Co-immunoprecipitation identified interaction between ACTL7B and the LC8 dynein light chains DYNLL1 and DYNLL2, which are first detected in step 9 spermatids and mislocalized when ACTL7B is absent. Our data unequivocally establishes that mutations in ACTL7B may be directly related to male infertility, pressing for additional research in men.

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Section: Resultsmentioning

confidence: 99%

Actl7b-deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis

Merges

Arévalo

Lohanadan

et al. 2022

Preprint

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“…In addition, at stage IX–XII, there were no significant differences between WT and Rhox8 KO, indicating a normal spermatogenesis during this period, which implies a crucial role of Rhox8 at the stage VII–VIII rather than at the later stages including spermiation. Taking into account that the action of retinoic acid is required for spermatogonial differentiation and that the level of retinoic acid is high at stage VII–VIII when type A spermatogonia differentiate into type A1 spermatogonia [48, 49], the highest level of Rhox8 expression at stage VII and VIII implies a possible role of Rhox8 in retinoic acid signaling pathway during spermatogonia differentiation.…”

Section: Discussionmentioning

confidence: 99%

Rhox8 homeobox gene ablation leads to rete testis abnormality and male subfertility in mice

Oh,

Kasu,

Bottoms

et al. 2023

Biology of Reproduction

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The reproductive homeobox X-linked (Rhox) genes encode transcription factors that are expressed selectively in reproductive tissues including the testis, epididymis, ovary, and placenta. While many Rhox genes are expressed in germ cells in the mouse testis, only Rhox8 is expressed exclusively in the Sertoli cells during embryonic and postnatal development, suggesting a possible role of Rhox8 in embryonic gonad development. Previously, Sertoli cell-specific knockdown of RHOX8 resulted in male subfertility due to germ cell defects. However, this knockdown model was limited in examining the functions of Rhox8 as RHOX8 knockdown occurred only postnatally, and there was still residual RHOX8 in the testis. In this study, we generated new Rhox8 knockout (KO) mice using the CRISPR/Cas9 system. Sex determination and fetal testis development were apparently normal in mutant mice. Fertility analysis showed a low fecundity in Rhox8 KO adult males, with disrupted spermatogenic cycles, increased germ cell apoptosis, and reduced sperm count and motility. Interestingly, Rhox8 KO testes showed an increase in testis size with dilated seminiferous tubules and rete testis, which might be affected by efferent duct Rhox8 ablation dysregulating the expression of metabolism and transport genes in the efferent ducts. Taken together, the data presented in this study suggest that Rhox8 in the Sertoli cells is not essential for sex determination and embryonic testis differentiation but has an important role in complete spermatogenesis and optimal male fertility.

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“…Moreover, ATRA is known to modulate uterine receptivity and facilitate embryo implantation by promoting the secretion of uterine factors necessary for embryo attachment and placental development [141,142]. Additionally, ATRA plays a pivotal role in male reproduction, influencing processes such as spermatogenesis, testicular development, and sperm production and motility [143,144].…”

Section: Growth Cellular Differentiation Morphogenesis and Reproducti...mentioning

confidence: 99%

Pet Wellness and Vitamin A: A Narrative Overview

Shastak,

Pelletier

2024

Animals

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The health of companion animals, particularly dogs and cats, is significantly influenced by nutrition, with vitamins playing a crucial role. Vitamin A, in particular, is indispensable, with diverse roles ranging from vision to immune modulation and reproduction. Despite its importance, the metabolism and dietary requirements of vitamin A in companion animals remain complex and not fully understood. This review provides a comprehensive overview of the historical perspective, the digestion, the metabolism, the physiological roles, the deficiency, the excess, and the interactions with other micronutrients of vitamin A in companion animals. Additionally, it highlights future research directions and gaps in our understanding. Insights into the metabolism of vitamin A in companion animals, personalized nutrition strategies based on genetic variability, longitudinal studies tracking the status of vitamin A, and investigations into its immunomodulatory effects are crucial for optimizing pet health and wellness. Furthermore, understanding the stability and bioavailability of vitamin A in pet food formulations is essential for ensuring the provision of adequate micronutrients. Overall, this review underscores the importance of vitamin A in companion animal nutrition and the need for further research to enhance our understanding and to optimize dietary recommendations for pet health and well-being.

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Cellular and molecular basis for the action of retinoic acid in spermatogenesis

Cited by 10 publications

References 58 publications

Actl7b-deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis

Actl7b-deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis

Rhox8 homeobox gene ablation leads to rete testis abnormality and male subfertility in mice

Pet Wellness and Vitamin A: A Narrative Overview

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