Cellular and mitochondrial determination of low molecular mass organic acids by LC–MS/MS

Fiori, Jessica; Amadesi, Elisa; Fanelli, Flaminia; Tropeano, Concetta Valentina; Rugolo, Michela; Gotti, Roberto

doi:10.1016/j.jpba.2017.11.071

Cited by 22 publications

(16 citation statements)

References 32 publications

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“…Similar to previous reports[46], fecal SCFA was measured by gas chromatography-mass spectrometry after derivatization reaction. Liquid chromatography-mass spectrometry was employed to quantify the levels of other organic acids, i.e ., lactate, pyruvate, and fumarate, as described previously[47].…”

Section: Methodsmentioning

confidence: 99%

Altered profiles of fecal metabolites correlate with visceral hypersensitivity and may contribute to symptom severity of diarrhea-predominant irritable bowel syndrome

Zhang¹,

Geng²,

Li³

et al. 2019

WJG

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BACKGROUNDFecal metabolites are associated with gut visceral sensitivity, mucosal immune function and intestinal barrier function, all of which have critical roles in the pathogenesis of irritable bowel syndrome (IBS). However, the metabolic profile and pathophysiology of IBS are still unclear. We hypothesized that altered profiles of fecal metabolites might be involved in the pathogenesis of IBS with predominant diarrhea (IBS-D).AIMTo investigate the fecal metabolite composition and the role of metabolites in IBS-D pathophysiology.METHODSThirty IBS-D patients and 15 age- and sex-matched healthy controls (HCs) underwent clinical and psychological assessments, including the IBS Symptom Severity System (IBS-SSS), an Italian modified version of the Bowel Disease Questionnaire, the Bristol Stool Form Scale (BSFS), the Hospital Anxiety and Depression Scale, and the Visceral Sensitivity Index. Visceral sensitivity to rectal distension was tested using high-resolution manometry system by the same investigator. Fecal metabolites, including amino acids and organic acids, were measured by targeted metabolomics approaches. Correlation analyses between these parameters were performed.RESULTSThe patients presented with increased stool water content, more psychological symptoms and increased visceral hypersensitivity compared with the controls. In fecal metabolites, His [IBS-D: 0.0642 (0.0388, 0.1484), HC: 0.2636 (0.0780, 0.3966), P = 0.012], Ala [IBS-D: 0.5095 (0.2826, 0.9183), HC: 1.0118 (0.6135, 1.4335), P = 0.041], Tyr [IBS-D: 0.1024 (0.0173, 0.4527), HC: 0.5665 (0.2436, 1.3447), P = 0.018], Phe [IBS-D: 0.1511 (0.0775, 0.3248), HC: 0.3967 (0.1388, 0.7550), P = 0.028], and Trp [IBS-D: 0.0323 (0.0001, 0.0826), HC: 0.0834 (0.0170, 0.1759), P = 0.046] were decreased in IBS-D patients, but isohexanoate [IBS-D: 0.0127 (0.0060, 0.0246), HC: 0.0070 (0.0023, 0.0106), P = 0.028] was significantly increased. Only Tyr was mildly correlated with BSFS scores in all subjects (r = -0.347, P = 0.019). A possible potential biomarker panel was identified to correlate with IBS-SSS score (R2Adjusted = 0.693, P < 0.001). In this regression model, the levels of Tyr, Val, hexanoate, fumarate, and pyruvate were significantly associated with the symptom severity of IBS-D. Furthermore, visceral sensation, including abdominal pain and visceral hypersensitivity, was correlated with isovalerate, valerate and isohexanoate.CONCLUSIONAltered profiles of fecal metabolites may be one of the origins or exacerbating factors of symptoms in IBS-D via increasing visceral sensitivity.

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Section: Methodsmentioning

confidence: 99%

Altered profiles of fecal metabolites correlate with visceral hypersensitivity and may contribute to symptom severity of diarrhea-predominant irritable bowel syndrome

Zhang¹,

Geng²,

Li³

et al. 2019

WJG

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“…23 However, these methods are time-consuming and reported in 13 to 75 min cycle times. 24,25 In regards of urinary organic acid profiles, the limit of detection, range and accuracy of our approach were good for routine laboratory practice.…”

Section: Resultsmentioning

confidence: 86%

Rapid Quantification of Urinary Organic Acids by HPLC Mass Spectrometry to Assess Nutritional Individuality in Chinese Children

Zhang

You

Zhu³

et al. 2020

ANAL. SCI.

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A urinary organic acids profile can be utilized as an effective screening tool for analyzing abnormality of nutrient metabolism. By using these metabolic markers in conjunction with one another, it helps in understanding how individual nutrient metabolism is executed and to determine where there may be imbalances in the metabolic cycle. In this study, we developed a rapid quantification method of 20 urinary organic acids by HPLC-mass spectrometry. A pre-analytical process of organic acid extraction from a urine sample is crucial in this methodology. The process was accomplished by liquid-liquid extraction followed by strong anion exchange. Compared with previous methods, this method greatly reduces the analysis time and allows for the simultaneous quantification of 20 organic acids within 10 min for the first time. This methodology enabled us to analyze urine samples collected from 34 Chinese children. The abnormalities of some urinary organic acids were found in this group, which revealed evidence of functional inadequacy of specific nutrients. The preliminary data in this study confirmed the suitability of the method for rapid and accurate quantification of the target organic acids in urine samples.

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“…The LOD and linearity range of HA were lower than those of reported methods [31, 32]. In addition, the linearity ranges and LODs of CA, MA, and SU were comparable or even lower than those of using HPLC–MS or colorimetric enzymatic methods [33, 34]. More importantly, the 2D analysis by HPLC–ESI–IMS substantially attenuates the interferences from crops, and therefore shows higher selectivity and easier manipulation relative to the reported methods.…”

Section: Resultsmentioning

confidence: 97%

A rapid liquid chromatography‐electrospray ionization‐ion mobility spectrometry method for monitoring nine representative metabolites in the seedlings of cucumber and wheat

Yuan

et al. 2020

J of Separation Science

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A fast and convenient high‐performance liquid chromatography‐electrospray ionization‐ion mobility spectrometry method was developed to determine nine representative metabolites in the seedlings of cucumber and wheat. The analytical conditions were obtained by optimizing the parameters of high‐performance liquid chromatography and ion mobility spectrometry. Briefly, acetonitrile–0.1% formic acid solution was selected as the mobile phase for gradient elution at a flow velocity of 0.4 mL/min. Under negative electrospray ionization mode, spray voltage of ion mobility spectrometry was 4.5 kV, and drift tube temperature was set at 90°C. The metabolites from seedling leaves were extracted using 80% acetonitrile as the solvent at 4°C for 12 h. Results showed that under soilless culture conditions, the contents of maltose, citric acid, and p‐hydroxybenzoic acid in the seedlings of cucumber and wheat were reduced by low concentration of itaconic acid, succinic acid, and citric acid. Importantly, this analytical approach demonstrated high sensitivity, good linear response, and high selectivity. The lowest limit of detection was 0.004 μg for p‐hydroxybenzoic acid. Overall, this high‐performance liquid chromatography‐electrospray ionization‐ion mobility spectrometry method is sensitive and efficient for rapid separation and identification of plant metabolites.

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Cellular and mitochondrial determination of low molecular mass organic acids by LC–MS/MS

Cited by 22 publications

References 32 publications

Altered profiles of fecal metabolites correlate with visceral hypersensitivity and may contribute to symptom severity of diarrhea-predominant irritable bowel syndrome

Altered profiles of fecal metabolites correlate with visceral hypersensitivity and may contribute to symptom severity of diarrhea-predominant irritable bowel syndrome

Rapid Quantification of Urinary Organic Acids by HPLC Mass Spectrometry to Assess Nutritional Individuality in Chinese Children

A rapid liquid chromatography‐electrospray ionization‐ion mobility spectrometry method for monitoring nine representative metabolites in the seedlings of cucumber and wheat

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