Cellular and Humoral Immunity against Cow's Milk Proteins in Type 1 Diabetes

Sarugeri, Elena; Dozio, Nicoletta; Meschi, Franco; Pastore, M. R.; Bonifacio, Ezio

doi:10.1006/jaut.1999.0327

Cited by 11 publications

(13 citation statements)

References 59 publications

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“…Increased cellular and humoral immune responses to different cow's milk proteins including BSA, -lactoglobulin and -casein have been reported in patients with type 1 diabetes (Karjalainen et al 1992, Cavallo et al 1996b, Vaarala et al 1996, Ellis et al 1998, but these results have not always been confirmed (Atkinson et al 1992, Roep et al 1999, Sarugeri et al 1999.…”

Section: Introductionmentioning

confidence: 99%

Establishment of T cell lines to bovine beta-casein and beta-casein-derived epitopes in patients with type 1 diabetes

Monetini

Barone²,

Stefanini³

et al. 2003

Journal of Endocrinology

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Enhanced cellular immune response to bovine -casein has been reported in patients with type 1 diabetes. In this study we aimed to establish -casein-specific T cell lines from newly diagnosed type 1 diabetic patients and to characterise these cell lines in terms of phenotype and epitope specificity. Furthermore, since sequence homologies exist between -casein and putative beta-cell autoantigens, reactivity to the latter was also investigated.T cell lines were generated from the peripheral blood of nine recent onset type 1 diabetic patients with different HLA-DQ and -DR genotypes, after stimulation with antigen pulsed autologous irradiated antigen presenting cells (APCs) and recombinant human interleukin-2 (rhIL-2). T cell line reactivity was evaluated in response to bovine -casein, to 18 overlapping peptides encompassing the whole sequence of -casein and to beta-cell antigens, including the human insulinoma cell line, CM, and a peptide from the beta-cell glucose transporter, GLUT-2. T cell lines specific to -casein could not be isolated from HLA-matched and -unmatched control subjects.-Casein T cell lines reacted to different sequences of the protein, however a higher frequency of T cell reactivity was observed towards the C-terminal portion (peptides B05-14, and B05-17 in 5/9 and 4/9 T cell lines respectively). Furthermore, we found that 1 out of 9 -casein-specific T cell lines reacted also to the homologous peptide from GLUT-2, and that 3 out of 4 of tested cell lines reacted also to extracts of the human insulinoma cell line, CM.We conclude that T cell lines specific to bovine -casein can be isolated from the peripheral blood of patients with type 1 diabetes; these cell lines react with multiple and different sequences of the protein particularly towards the C-terminal portion. In addition, reactivity of -casein T cell lines to human insulinoma extracts and GLUT-2 peptide was detected, suggesting that the potential cross-reactivity with beta-cell antigens deserves further investigation.

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Section: Introductionmentioning

confidence: 99%

Establishment of T cell lines to bovine beta-casein and beta-casein-derived epitopes in patients with type 1 diabetes

Monetini

Barone²,

Stefanini³

et al. 2003

Journal of Endocrinology

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“…In type I diabetic patients, much attention has been given to infant's early diet [8,9] and in particular to a possible role of cow's milk [10]. Sarugeri et al [11] suggests that humoral responses to a-and b-casein are elevated in Italian patients with type I diabetes.There are few studies of caseins in milk using 2- 24] and only for human and bovine milk; thus, the aim of this work was to detect, by 2-DE coupled with MALDI-TOF mass spectrometry, two types of genotyped goat milk. These milk samples have defined characteristics.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Identification of caseins in goat milk

et al. 2002

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The importance of goat milk in infant diet is growing, because it is reported that goat's milk in some cases is less allergenic than cow's milk. This is due probably to the lower presence of caseins associated with a specific type of alpha(s1)-casein. In caprine breeds, four types of alpha(s1)-casein alleles are identified and associated with various amounts of this protein in milk. The contribution of strong alleles to the goat milk is approximately 3.6 g/L of alpha(s1)-casein, while for middle alleles is only 1.6 g/L, weak alleles 0.6 g/L. The contribution of null allele is very low (or non-existent). The quantity of total caseins in caprine milk is positively correlated with the amount of alpha(s1)-casein. Milk from animals possessing strong alleles contain significantly more total caseins than milk from animals without those alleles. This is important because animals with mild alleles can be employed to produce milk for allergic subjects while the other animals can be used to produce milk for the dairy industry. This work shows casein profiles of two types of classified goat milk (B, strong alpha(s1) allele, 0, null alpha(s1) allele) with two-dimensional electrophoresis coupled with matrix-assisted laser desorption/ionization-time of flight mass spectrometry, and it confirms the different polymorphisms at locus alpha(s1) casein.

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“…Case-control studies have also found associations between cow's milk antibodies and T1D see e.g. (Sarugeri et al, 1999;Monetini et al, 2002), but some form of reverse causality cannot be excluded as alternative explanations for the association described in these studies. Although an early study indicated a role of so-called molecular mimicry between a protein in cow's milk and a -cell antigen (Karjalainen et al, 1992), it was subsequently refuted (Rønningen et al, 1998).…”

Section: Breast-feeding and Cow's Milkmentioning

confidence: 75%

Genetic Testing of Newborns for Type 1 Diabetes Susceptibility – The MIDIA Study

Rønningen¹

2011

Type 1 Diabetes - Complications, Pathogenesis, and Alternative Treatments

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Cellular and Humoral Immunity against Cow's Milk Proteins in Type 1 Diabetes

Cited by 11 publications

References 59 publications

Establishment of T cell lines to bovine beta-casein and beta-casein-derived epitopes in patients with type 1 diabetes

Establishment of T cell lines to bovine beta-casein and beta-casein-derived epitopes in patients with type 1 diabetes

Identification of caseins in goat milk

Genetic Testing of Newborns for Type 1 Diabetes Susceptibility – The MIDIA Study

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