Cellular and humoral immune responses to recombinant antigens in sheep infected with <i>Toxoplasma gondii</i>

Coughlan, S. N.; Saman, Eric; Jacobs, Dirk; Mercier, Corinne; Cesbron-Delauw, M.-F.; Trees, A. J.

doi:10.1111/j.1365-3024.1995.tb00916.x

Cited by 9 publications

(3 citation statements)

References 17 publications

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“…Nevertheless infection with tissue cysts can occur, although rare, when sheep eat the placenta or abortion products from ewes that aborted as a result of toxoplasmosis [ 47 - 49 ]. As mentioned above, only a few studies analyzed the immune response in sheep, three of them after oral inoculation with oocysts [ 42 , 45 , 50 ] and four following an intraperitoneal injection with tachyzoites of the RH strain or S48 strain [ 17 , 51 - 53 ]. The latter is a non-natural infection.…”

Section: Discussionmentioning

confidence: 99%

Parasite distribution and associated immune response during the acute phase of Toxoplasma gondiiinfection in sheep

Verhelst

Craeye

Entrican³

et al. 2014

BMC Vet Res

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BackgroundIn many countries, Toxoplasma gondii (T. gondii) is a major cause of reproductive disorders and abortions in the sheep industry, and therefore responsible for important financial and economic losses. In addition, undercooked infected lamb is an important risk factor for human toxoplasmosis.In the present study, the initial phase of the infection was investigated: the parasite’s entry site, the subsequent distribution of the parasite and the host-immune response.ResultsParasite DNA was already detected in the cranial small intestinal mucosa the first days after oral infection with T. gondii tissue cysts. Simultaneously, high IFN-gamma and IL-12 responses were induced mainly in the mesenteric lymph nodes. The emergence of IgG1 (at 8dpi), and IgG2 (at 11 dpi) was accompanied by a decrease or even disappearance of the IFN-gamma and IL-12 response in the Peyers’ patches (PP), PBMC’s and popliteal LN’s. Meanwhile the parasite DNA could be recovered from most mucosal and systemic tissues to become undetectable in the small intestine, popliteal LN, PBMC and spleen 3 weeks pi.ConclusionsOur results indicate that parasites enter the cranial small intestine the first days after infection and that after an increase the first two weeks after infection, the parasite DNA levels in the intestine drop below the detection limit three weeks after infection. This coincides with an increase in parastic-specific serum IgG1 and IgG2 and a decrease of the antigen-specific IFN-gamma response in PP, PBMC and popliteal LN. We suggest a role for IFN-gamma and IL-12 in controlling the infection.

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Section: Discussionmentioning

confidence: 99%

Parasite distribution and associated immune response during the acute phase of Toxoplasma gondiiinfection in sheep

Verhelst

Craeye

Entrican³

et al. 2014

BMC Vet Res

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“…This may be attributed to its sensitivity against host proteases. Nevertheless, truncated forms of ROP2, expressed as fusion with a Cro-LacI peptide or with a six histidinyl residues tag (11,12), were purified and used to study, in diagnostic tests, the humoral response during Toxoplasma infection of humans.…”

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confidence: 99%

Expression of a Recombinant Toxoplasma gondii ROP2 Fragment as a Fusion Protein in Bacteria Circumvents Insolubility and Proteolytic Degradation

Jacquet

Daminet

Haumont

et al. 1999

Protein Expression and Purification

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“…Evidence for the compartmentalized clonal expansion of B cells based on PCR analysis was reported for the brains of multiple sclerosis patients (12) and the salivary glands of patient with Sjögren's syndrome (14,15). Secondary gene rearrangements, involving V H replacements were found in clonally related B cells that expanded locally in the synovial tissues of patients with rheumatoid arthritis (32). High Ag load may be the driving force for localized B cell expansion.…”

Section: Figurementioning

confidence: 98%

Evidence for a Compartmentalized B Cell Response as Characterized by IgG Epitope Specificity in Human Ocular Toxoplasmosis

Klaren

Peek

2001

The Journal of Immunology

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Infectious agents in the eye induce both a local and a systemic humoral immune response. Previously, differences in Ag recognition were observed between systemic and ocular derived IgG of patients with ocular toxoplasmosis. This finding implied a nonrandom distribution of IgG-producing B cells in the inflamed eye. In the present study, we compared the intraocular and systemic B cell responses of patients with ocular toxoplasmosis to a single Toxoplasma gondii Ag. Two series of C-terminally deleted recombinant T. gondii GRA-2 proteins were constructed to delineate IgG B cell epitopes of paired ocular and serum samples. Differences in epitope region recognition between the ocular and systemic compartment were detected in 9 of 13 patients. The difference in distribution of GRA-2 epitopes between paired samples is indicative of a local GRA-2 specific B cell population functionally different from the systemic GRA-2-specific B cell population. Our results suggest a selective activation of a subset of B cells locally in nonlymphoid tissue.

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Cellular and humoral immune responses to recombinant antigens in sheep infected with Toxoplasma gondii

Cited by 9 publications

References 17 publications

Parasite distribution and associated immune response during the acute phase of Toxoplasma gondiiinfection in sheep

Parasite distribution and associated immune response during the acute phase of Toxoplasma gondiiinfection in sheep

Expression of a Recombinant Toxoplasma gondii ROP2 Fragment as a Fusion Protein in Bacteria Circumvents Insolubility and Proteolytic Degradation

Evidence for a Compartmentalized B Cell Response as Characterized by IgG Epitope Specificity in Human Ocular Toxoplasmosis

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