Cellular and Humoral Immune Responses to Pandemic Influenza Vaccine in Healthy and in Highly Active Antiretroviral Therapy-Treated HIV Patients

Agrati, Chiara; Gioia, Cristiana; Castilletti, Concetta; Lapa, Daniele; Berno, Giulia; Puro, Vincenzo; Carletti, Fabrizio; Cimini, Eleonora; Nisii, Carla; Castellino, Flora; Martini, Federico; Capobianchi, Maria Rosaria

doi:10.1089/aid.2011.0371

Cited by 12 publications

(13 citation statements)

References 52 publications

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“…Although some studies demonstrated that HIV+ patients have similar or greater responses to the influenza vaccine [31,38,39], most reported that these patients have compromised responses to this vaccine [5,17-19,22,40], in disagreement with our results. Potential reasons for this discrepancy may include differences in patient populations.…”

Section: Discussioncontrasting

confidence: 99%

Key differences in B cell activation patterns and immune correlates among treated HIV-infected patients versus healthy controls following influenza vaccination

Luo

Zhang

Martin

et al. 2016

Vaccine

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Background There is increasing recognition of the role of B cell dysfunction in HIV pathogenesis, but little is known about how these perturbations may influence responses to vaccinations. Methods Healthy controls (n = 16) and antiretroviral therapy (ART)-treated aviremic HIV-infected subjects (n = 26) receiving standard-of-care annual influenza vaccinations were enrolled in the present study. Total bacterial 16S rDNA levels were assessed by quantitative polymerase chain reactions in plasma. Serologic responses were characterized by ELISA, hemagglutination inhibition assay (HI), and microneutralization, and cell-mediated responses were assessed by ELISPOT (antigen-specific IgG+ antibody-secreting cells (ASCs)) and flow cytometry at pre-vaccination (D0), day 7-10 (D7) and day 14-21 (D14) post-vaccination. Results Decreased peripheral CD4+ T cell absolute counts and increased frequencies of cycling and apoptotic B cells were found at baseline in HIV-infected subjects relative to healthy controls. In healthy controls, post-vaccination neutralizing activities were related to the frequencies of vaccine-mediated apoptosis and cycling of B cells, but not to CD4+ T cell counts. In patients, both baseline and post-vaccination neutralizing activities were directly correlated with plasma level of bacterial 16S rDNA. However, overall vaccine responses including antibody titers and fold changes were comparable or greater in HIV-infected subjects relative to healthy controls. Conclusion B cell function correlates with measures of recall humoral immunity in response to seasonal influenza vaccination in healthy controls but not in ART-treated patients.

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Section: Discussioncontrasting

confidence: 99%

Key differences in B cell activation patterns and immune correlates among treated HIV-infected patients versus healthy controls following influenza vaccination

Luo

Zhang

Martin

et al. 2016

Vaccine

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show abstract

“…There was no significant difference between HIV-negative and HIV-positive cohorts in HI titers to any of the four influenza virus strains tested either at baseline or after vaccination. This finding contrasts with previous studies on HI responses in HIV-infected subjects (18,(20)(21)(22)(23) and likely reflects the generally immunocompetent nature and high CD4 T cell counts of our HIV-positive cohort (24,25).…”

Section: Influenza Vaccination Cohortscontrasting

confidence: 99%

Antibody Responses with Fc-Mediated Functions after Vaccination of HIV-Infected Subjects with Trivalent Influenza Vaccine

Kristensen

Lay

Ana-Sosa-Batiz

et al. 2016

J Virol

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“…Similarly, the establishment of influenza vaccine-elicited memory B cell responses, as measured by polyclonal stimulation and B cell ELISpot, are diminished compared to healthy controls 12 . Initiation of effective ART can restore serological responses to influenza vaccines to levels comparable with healthy controls 32 33 . However it remains unclear if dysfunction or depletion of antigen-specific memory B cell populations in ART-controlled infection affects vaccine-elicited immunity against influenza.…”

mentioning

confidence: 99%

HIV-dependent depletion of influenza-specific memory B cells impacts B cell responsiveness to seasonal influenza immunisation

Wheatley

Kristensen

Lay

et al. 2016

Sci Rep

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Infection with HIV drives significant alterations in B cell phenotype and function that can markedly influence antibody responses to immunisation. Anti-retroviral therapy (ART) can partially reverse many aspects of B cell dysregulation, however complete normalisation of vaccine responsiveness is not always observed. Here we examine the effects of underlying HIV infection upon humoral immunity to seasonal influenza vaccines. Serological and memory B cell responses were assessed in 26 HIV+ subjects receiving ART and 30 healthy controls immunised with the 2015 Southern Hemisphere trivalent inactivated influenza vaccine (IIV3). Frequencies and phenotypes of influenza hemagglutinin (HA)-specific B cells were assessed by flow cytometry using recombinant HA probes. Serum antibody was measured using hemagglutination inhibition assays. Serological responses to IIV3 were comparable between HIV+ and HIV− subjects. Likewise, the activation and expansion of memory B cell populations specific for vaccine-component influenza strains was observed in both cohorts, however peak frequencies were diminished in HIV+ subjects compared to uninfected controls. Lower circulating frequencies of memory B cells recognising vaccine-component and historical influenza strains were observed in HIV+ subjects at baseline, that were generally restored to levels comparable with HIV− controls post-vaccination. HIV infection is therefore associated with depletion of selected HA-specific memory B cell pools.

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Cellular and Humoral Immune Responses to Pandemic Influenza Vaccine in Healthy and in Highly Active Antiretroviral Therapy-Treated HIV Patients

Cited by 12 publications

References 52 publications

Key differences in B cell activation patterns and immune correlates among treated HIV-infected patients versus healthy controls following influenza vaccination

Key differences in B cell activation patterns and immune correlates among treated HIV-infected patients versus healthy controls following influenza vaccination

Antibody Responses with Fc-Mediated Functions after Vaccination of HIV-Infected Subjects with Trivalent Influenza Vaccine

HIV-dependent depletion of influenza-specific memory B cells impacts B cell responsiveness to seasonal influenza immunisation

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