Cellular and humoral components of bronchoalveolar lavage in the sheep

Rola‐Pleszczynski, Marek; Sirois, Pierré; Bégin, Raymond

doi:10.1007/bf02713902

Cited by 23 publications

(1 citation statement)

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“…'2 BRONCHOALVEOLAR LAVAGE FLUID ANALYSIS Most of the techniques used-in bronchoalveolar lavage procedures and analyses have been previously described. '2 17 Briefly, lung lavage was performed by slow infusion of three 50-ml 39°C aliquots of phosphate-buffered saline through a 50-ml Luerlock syringe attached to the work channel of the bronchoscope and by gentle syringe aspiration of the effluent. The effluent was passed through four layers of cheesecloth to remove mucus and the cells were The yield of lung biopsy specimens was similar to that of our previous study and the histopathological features of the control material remained normal (fig 1, panel a).…”

Section: Experimental Designmentioning

confidence: 99%

Assessment of progression of asbestosis in the sheep model by bronchoalveolar lavage and pulmonary function tests.

Bégin¹,

Rola‐Pleszczynski²,

Massé³

et al. 1983

Thorax

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To study the relationship between the results of bronchoalveolar lavage and pulmonary function tests during induction and progression of asbestosis, three groups of six sheep were exposed repeatedly by intratracheal injection to either saline (controls), low doses of Canadian chrysotile UICC asbestos (cumulative exposure 328 mg) (low-dose group), or high doses of the same fibres (cumulative dose 2282 mg) (high-dose group) until there was clear evidence of alveolitis from the lung biopsy specimens of all sheep of the high-dose group. During the course of this induction and for the following eight months lung biopsies, bronchoalveolar lavage and pulmonary function tests were performed at two-month intervals. At the time of initial alveolitis in the high-dose group there was no significant change in the cellularity of the bronchoalveolar lavage fluid, but static lung compliance (C5t), vital capacity (VC), arterial oxygen tension (Pao2), and diffusion capacity (DL/VA) were significantly lower than in the other groups. In the following months, as the alveolitis evolved into a fibrosing process, macrophages and neutrophils from the bronchoalveolar lavage fluid increased significantly and pulmonary function deteriorated. Proteins and enzymes in the bronchoalveolar lavage fluid also increased significantly in the high-dose group. These data show that in the sheep model of asbestosis simple tests of pulmonary function correlate well with histological changes and changes in the bronchoalveolar lavage fluid in the course of the disease and can be used to assess progression of asbestosis.

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Section: Experimental Designmentioning

confidence: 99%