Cellular and Chemical Assays for Discovery of Novel Antioxidants in Marine Organisms

Hofer, Tim; Eriksen, Tonje Engevik; Hansen, Espen; Varmedal, Ingrid; Jensen, Ida‐Johanne; Hammer-Andersen, Jeanette; Olsen, Ragnar L.

doi:10.1007/978-1-60761-956-7_32

Cited by 7 publications

(18 citation statements)

References 62 publications

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“…Wolfe and co-workers classifies them as having a moderate effect in the ORAC assay [20], it has also been reported by our research group [21]. When examined in this study, quercetin and luteolin displayed activities of 7 and 10 µM Trolox equivalents (TE), respectively, at 1 μg/mL.…”

Section: Resultssupporting

confidence: 77%

“…These two known antioxidants have previously been reported to have a high antioxidant activity in the CAA assay, both by our research group and others [20,21]. As seen by Figure 2, compound 2 shows a dose-dependent activity, capable of inhibiting oxidation at concentrations as low as 10 µg/mL (68%).…”

Section: Resultsmentioning

confidence: 61%

“…The use of cellular assays in addition to biochemical ones have also previously been utilized by our research group [21]. Information obtained by the cellular assays is advantageous, as biological systems are much more complex than chemical mixtures.…”

Section: Resultsmentioning

confidence: 99%

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Cellular Antioxidant Effect of Four Bromophenols from the Red Algae, Vertebrata lanosa

et al. 2013

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Three known bromophenols, 2,3-dibromo-4,5-dihydroxybenzylaldehyde (1), 2,2′,3-tribromo-3′,4,4′,5-tetrahydroxy-6′-hydroxymethyldiphenylmethane (2) and bis(2,3-dibromo-4,5-dihydroxylbenzyl) ether (3), and one new one, 5,5″-oxybis(methylene)bis(3-bromo-4-(2′,3′-dibromo-4′,5′-dihydroxylbenzyl)benzene-1,2-diol) (4), were isolated from an extract of the red alga, Vertebrata lanosa. The antioxidant activity of these four bromophenols was examined using one biochemical and two cellular assays: Oxygen Radical Absorbance Capacity (ORAC), Cellular Antioxidant Activity (CAA) and Cellular Lipid Peroxidation Antioxidant Activity (CLPAA) assays. Compound 2 distinguished itself by showing potent activity, having a better antioxidant effect than luteolin in both the CAA and CLPAA assays and of quercetin in the CLPAA assay. Although several bromophenols are known to be potent antioxidants in biochemical assays, this is the first time their cellular antioxidant activity has been demonstrated.

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Section: Resultssupporting

confidence: 77%

Section: Resultsmentioning

confidence: 61%

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Cellular Antioxidant Effect of Four Bromophenols from the Red Algae, Vertebrata lanosa

et al. 2013

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“…We used the cellular antioxidant activity assay (CAA, Figure 4) and the cellular lipid peroxidation antioxidant activity (CLPAA, Figure 5) assays [20,21] to measure the intracellular reactive oxygen species (ROS) and the lipid membrane antioxidant activity, respectively. None of the molecules had any effect in the CAA assay (Figure 4).…”

Section: Resultsmentioning

confidence: 99%

“…The CLPAA assay on the other hand detects antioxidants preventing lipid peroxidation in cellular membranes by monitoring the increase of a green fluorescence product produced by the lipophilic probe C-11-BODIPY after addition of cumene hydroperoxide (CumOOH) [21]. …”

Section: Resultsmentioning

confidence: 99%

Antioxidant and Anti-Inflammatory Activities of Barettin

et al. 2013

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In this paper, we present novel bioactivity for barettin isolated from the marine sponge Geodia barretti. We found that barettin showed strong antioxidant activity in biochemical assays as well as in a lipid peroxidation cell assay. A de-brominated synthetic analogue of barettin did not show the same activity in the antioxidant cell assay, indicating that bromine is important for cellular activity. Barettin was also able to inhibit the secretion of the inflammatory cytokines IL-1β and TNFα from LPS-stimulated THP-1 cells. This combination of anti-inflammatory and antioxidant activities could indicate that barettin has an atheroprotective effect and may therefore be an interesting product to prevent development of atherosclerosis.

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