Cellular alterations in midgut cells of honey bee workers (Apis millefera L.) exposed to sublethal concentrations of CdO or PbO nanoparticles or their binary mixture

Dabour, Khaled; Naggar, Yahya Al; Masry, Saad; Naiem, Elsaied; Giesy, John P.

doi:10.1016/j.scitotenv.2018.09.311

Cited by 48 publications

(37 citation statements)

References 80 publications

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“…In the current study, short-term exposure of adult D. melanogaster to a sublethal concentration of CdO NPs adversely affected all studied life history traits of the fly including survival, developmental time and reproductive fitness. Such results are in line with previous studies revealing that exposure to Cd, either as salts (CdCl2) or as nano-sized (CdO) can cause toxic effects in D. melanogaster as well as organisms (AL Naggar et al, 2018; Bixler and Schnee, 2018; Blum et al, 2012; Dabour et al, 2019; Hu et al, 2019; Mathew and NB, 2018). Inhalation of CdO NPs during pregnancy negatively affects fecundity and inhibits the development of fetal and postnatal offspring of mice (Blum et al, 2012).…”

Section: Discussionsupporting

confidence: 92%

“…Exposure of aquatic and terrestrial organisms to potentially toxic metals and metalloids can have adverse effects (Ali et al, 2019). Even at sublethal concentrations, metals and metalloids can cause toxic effects (AL Naggar et al, 2018; Dabour et al, 2019). Given a substantial amount of existing literature on potential risks associated with exposure to emerging nanomaterials (Exbrayat et al, 2015), to our knowledge, this is the first study to investigate and report adverse long lasting effects on key life history traits of D. melanogaster well beyond exposure times to CdO NPs even at a lesser concentration.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have demonstrated toxic potencies of cadmium-containing nanoparticles (NPs) in vitro and in vivo (AL Naggar et al, 2018; Brunetti et al, 2013; Dabour et al, 2019; Demir et al, 2020) and on human health (Blum et al, 2012; Dumkova et al, 2016b). Although these studies provide independent empirical support for the toxicity of these Cd-containing NPs, however more information about potential risks of these metallic nanoparticles on living organisms are still required; such as for example their effects on life history traits, possible short or long term effects on physiology at the genomic and proteomic levels, during and after exposure.…”

Section: Introductionmentioning

confidence: 99%

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Consequences of a short-term exposure to a sub lethal concentration of CdO nanoparticles on key life history traits in the fruit fly (Drosophila melanogaster)

El-Kholy

Giesy

Naggar

2020

Preprint

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Nanoparticles of cadmium oxide (CdO NPs) are among the most common industrial metal oxide nanoparticles. Early adulthood (F0) fruit flies (D. melanogaster) were exposed for 7 days to a sub lethal concentration (0.03 mg CdO NPs/ml, which was 20% of the LC50), spiked into food media to test for long term-effects over time and beyond their direct exposure on key life history traits. Effects on survival, developmental time, eclosion rate, fecundity and negative geotaxis performance were assessed. Potential effects on ultrastructure of mid gut cells were also investigated by use of electron microscopy. All studied life history traits, as well as climbing behavior were adversely affected by exposure to CdO NPs. In non-exposed progeny (F1) of adult flies (F0), a blistered wing phenotype was also observed. Lysis of nuclear and rough endoplasmic reticulum (rER) membranes, mitochondrial swelling and lysis were among the most common cellular alterations observed in midgut cells of F0 flies exposed to CdO NPs. Genes encoding for metallothionein (MTn A-D) were significantly upregulated in both parent flies (F0) and their progeny (F1) after exposure of F0 flies to CdO NPs, compared to unexposed, control flies, a result which indicated potential, long-term effects. Taken together, these results suggest that short-term exposure to a sublethal concentration of CdO NPs is sufficient to cause long-lasting, harmful effects on fruit flies.Graphical Abstract

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Consequences of a short-term exposure to a sub lethal concentration of CdO nanoparticles on key life history traits in the fruit fly (Drosophila melanogaster)

El-Kholy

Giesy

Naggar

2020

Preprint

Self Cite

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“…ehabsoft.com/ldpline)) ( Table 1), and the cumulative death of the beetles was calculated for the tested doses (S1 Table). All the tested parameters were assessed in those beetles injected with the sublethal dose of 0.01 mg/g (Group 2) [35].…”

Section: Exposure Protocol and Effects On Survivalmentioning

confidence: 99%

Relative expression of microRNAs, apoptosis, and ultrastructure anomalies induced by gold nanoparticles in Trachyderma hispida (Coleoptera: Tenebrionidae)

et al. 2020

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The extensive use of nanomaterials generates toxic effects on non-target species and the ecosystem. Although gold nanoparticles (Au-NPs) are generally expected to be safe, the recent study contains conflicting data regarding their cytotoxicity in the darkling beetles Trachyderma hispida. The study postulated cellular perturbation in the ovarian tissue of the beetles induced by a sublethal dose of Au-NPs (0.01 mg/g). When compared with the controls, a significant inhibition in the activities of the antioxidant enzymes selenium-dependent (GPOX) and selenium-independent (GSTP) glutathione peroxidases (GPx) was observed in the treated beetles. The study proposed microRNAs (miRNA-282 and miRNA-989) as genotoxic markers for the first time, reporting a significant suppression in their transcriptional levels in the treated beetles. Furthermore, TUNEL (Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) and flow cytometry assays (annexin V-Fitc) indicated a significant increase in ovarian cell apoptosis in the treated beetles. Additionally, an ultrastructure examination revealed pathological changes in the ovarian cells of the treated beetles. The resulting anomalies in the present study may interrupt the fecundity of the beetles and lead to the future suppression of beetle populations.

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“…Also, cell nuclei contained a higher amount and density of heterochromatin [80]. Honeybees fed with food contaminated with sublethal concentrations of heavy metals (Cadmium oxide (CdO) and lead oxide (PbO)) showed similar cellular damage in the midgut tissue and a disruption of the peritrophic membrane [213]. Damage of the peritrophic membrane, the midgut tissue, or an altered gut microbiome due to the uptake of heavy metal may increase the susceptibility of social insects to gut pathogens such as Nosema spp.…”

Section: Pollutants: Heavy Metalsmentioning

confidence: 99%

Pollutants and Their Interaction with Diseases of Social Hymenoptera

Feldhaar

Otti

2020

Insects

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Many insect species, including social insects, are currently declining in abundance and diversity. Pollutants such as pesticides, heavy metals, or airborne fine particulate matter from agricultural and industrial sources are among the factors driving this decline. While these pollutants can have direct detrimental effects, they can also result in negative interactive effects when social insects are simultaneously exposed to multiple stressors. For example, sublethal effects of pollutants can increase the disease susceptibility of social insects, and thereby jeopardize their survival. Here we review how pesticides, heavy metals, or airborne fine particulate matter interact with social insect physiology and especially the insects’ immune system. We then give an overview of the current knowledge of the interactive effects of these pollutants with pathogens or parasites. While the effects of pesticide exposure on social insects and their interactions with pathogens have been relatively well studied, the effects of other pollutants, such as heavy metals in soil or fine particulate matter from combustion, vehicular transport, agriculture, and coal mining are still largely unknown. We therefore provide an overview of urgently needed knowledge in order to mitigate the decline of social insects.

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Cellular alterations in midgut cells of honey bee workers (Apis millefera L.) exposed to sublethal concentrations of CdO or PbO nanoparticles or their binary mixture

Cited by 48 publications

References 80 publications

Consequences of a short-term exposure to a sub lethal concentration of CdO nanoparticles on key life history traits in the fruit fly (Drosophila melanogaster)

Consequences of a short-term exposure to a sub lethal concentration of CdO nanoparticles on key life history traits in the fruit fly (Drosophila melanogaster)

Relative expression of microRNAs, apoptosis, and ultrastructure anomalies induced by gold nanoparticles in Trachyderma hispida (Coleoptera: Tenebrionidae)

Pollutants and Their Interaction with Diseases of Social Hymenoptera

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