Cellular adhesion is required for effector functions of human eosinophils via G-protein coupled receptors

Fujiu, T; Katô, Masahiko; Kimura, Hirokazu; Tachibana, Akira; Suzuki, Masato; Nako, Yasushi; Morikawa, Akihiro

doi:10.1016/s1081-1206(10)61917-5

Cited by 14 publications

(12 citation statements)

References 39 publications

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“…Because Ca 2+ mobilization is not rate limiting for degranulation of suspended cells (Pierini et al, 1997), other aspects of signaling evidently provide this kinetic regulation that depends on cell adherence and morphology. A role for cell adherence in stimulated exocytosis was demonstrated previously for human eosinophils (Fujiu et al, 2002), as well as for RBL mast cells (Apgar, 1997), but the time dependence of this enhancement was not previously characterized. Although the molecular basis for the time needed for cell attachment to achieve optimal degranulation of RBL mast cells remains to be determined, it could be related to the time needed for development of stable cell protrusions.…”

Section: Degranulation Is Significantly Influenced By the Duration Ofmentioning

confidence: 87%

Spatiotemporal Resolution of Mast Cell Granule Exocytosis

Cohen

Corwith

Holowka

et al. 2012

Journal of Cell Science

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SummaryMast cell activation initiated by antigen-mediated crosslinking of IgE receptors results in stimulated exocytosis of secretory lysosomes in the process known as degranulation. Much has been learned about the molecular mechanisms important for this process, including the crucial role of Ca 2+ mobilization, but spatio-temporal relationships between stimulated Ca 2+ mobilization and granule exocytosis are incompletely understood. Here we use a novel imaging-based method that uses fluorescein isothiocyanate (FITC)-dextran as a reporter for granule exocytosis in RBL mast cells and takes advantage of the pH sensitivity of FITC. We demonstrate the selectivity of FITCdextran, accumulated by fluid-phase uptake, as a marker for secretory lysosomes, and we characterize its capacity to delineate different exocytotic events, including full fusion, kiss-and-run transient fusion and compound exocytosis. Using this method, we find strong dependence of degranulation kinetics on the duration of cell to substrate attachment. We combine imaging of degranulation and Ca 2+ dynamics to demonstrate a spatial relationship between the sites of Ca 2+ wave initiation in extended cell protrusions and exocytosis under conditions of limited antigen stimulation. In addition, we find that the spatially proximal Ca 2+ signaling and secretory events correlate with participation of TRPC1 channels in Ca 2+ mobilization.

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Section: Degranulation Is Significantly Influenced By the Duration Ofmentioning

confidence: 87%

Spatiotemporal Resolution of Mast Cell Granule Exocytosis

Cohen

Corwith

Holowka

et al. 2012

Journal of Cell Science

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“…Like neutrophils, eosinophils require b 2 -integrin participation for activation of the respiratory burst and degranulation. 10,11 We have previously reported that VCAM-1-activated eosinophil O 2 2 generation is completely inhibited by anti-b2 mAb but only partially inhibited by anti-a4 19 and now WAY103. Activation of VLA-4 has been proposed as a mechanism of inside-out cell signaling, which activates b 2 -integrins 30,31 and hence b 2 -dependent functions, such as transendothelial migration and respiratory burst.…”

Section: Discussionmentioning

confidence: 98%

“…Eosinophil adhesion to VCAM-1 can both stimulate superoxide anion (O 2 2 ) generation and potentiate activation by N-formyl-methionylleucyl-phenylalanine, 8 whereas eosinophil degranulation is strongly dependent on cell adhesion 9 ; both of these functions require activation of eosinophil b 2 -integrin similar to neutrophils. 10,11 Therefore agents that modulate eosinophil adhesion might inhibit not only a cell's migration to the airway but also its functional activation once it arrives. A better understanding of the effects of VLA-4 antagonists on cell functions must be gained before these agents can be used therapeutically.…”

mentioning

confidence: 99%

Effects of the very late adhesion molecule 4 antagonist WAY103 on human peripheral blood eosinophil vascular cell adhesion molecule 1–dependent functions

Sedgwick

Jansen

Kennedy³

et al. 2005

Journal of Allergy and Clinical Immunology

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“…Appropriate stimuli such as immunoglobulins [4], cytokines [5] and lipid mediators, including leukotrienes [6] and platelet-activating factor [7], induce degranulation of toxic eosinophil granule proteins. Subsequent tissue damage induced by eosinophil granule proteins and superoxides contributes to the airway inflammation associated with such diseases [3,8].…”

Section: Introductionmentioning

confidence: 99%

Elevated Macrophage Inflammatory Protein 1α and Interleukin-17 Production in an Experimental Asthma Model Infected with Respiratory Syncytial Virus

Ishioka

Yamada²,

Kimura

et al. 2013

Int Arch Allergy Immunol

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Background: Respiratory syncytial virus (RSV) infection is associated with both the development and exacerbation of bronchial asthma. We examined eosinophil infiltration and the cytokine profiles of both airway and peripheral blood in antigen-sensitized mice infected with RSV to investigate the pathogenesis of exacerbations of asthma due to RSV infection. Methods: Ovalbumin (OVA)-sensitized mice were challenged by OVA inhalation 3 times and then infected with RSV [10⁵ TCID₅₀ (50% of tissue culture infectious dose)/25 g body weight] or mock infection immediately after the last challenge. Animals from each group, namely, the control (PBS instead of OVA inhalation plus mock infection), RSV (PBS plus RSV), OVA (OVA plus mock) and OVA/RSV (OVA plus RSV) were analyzed. Analysis included evaluation of airway responsiveness to methacholine, pathological findings in the airway by hematoxylin and eosin (HE) and Luna staining, bronchoalveolar fluid (BALF) and peripheral leukocytes counts, and concentrations of multiple cytokines/chemokines in both BALF and serum. Results: Airway responsiveness was significantly enhanced in the OVA and OVA/RSV groups compared with the control group. Levels of tissue and BALF eosinophils were higher in the OVA and OVA/RSV groups than in the RSV or control group. Significantly higher levels of macrophage inflammatory protein (MIP)-1α in BALF were observed in the OVA/RSV group compared with the 3 other groups. Production of serum IL-17 was also significantly elevated in the OVA/RSV group compared with the control or OVA group. Conclusions: These findings suggest that MIP-1α and IL-17 may play important roles in acute exacerbation of asthma induced by RSV in an animal model.

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Cellular adhesion is required for effector functions of human eosinophils via G-protein coupled receptors

Cited by 14 publications

References 39 publications

Spatiotemporal Resolution of Mast Cell Granule Exocytosis

Spatiotemporal Resolution of Mast Cell Granule Exocytosis

Effects of the very late adhesion molecule 4 antagonist WAY103 on human peripheral blood eosinophil vascular cell adhesion molecule 1–dependent functions

Elevated Macrophage Inflammatory Protein 1α and Interleukin-17 Production in an Experimental Asthma Model Infected with Respiratory Syncytial Virus

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