Cellular activity of osteoblasts in the presence of hydroxyapatite: an in vitro experiment

Alliot‐Licht, Brigitte; Grégoire, Marc; Orly, I.; Ménanteau, Jean

doi:10.1016/0142-9612(91)90025-6

Cited by 71 publications

(31 citation statements)

References 33 publications

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“…It has been suggested that the low ALP-specific activity and 1,25 (OH 2 ) vitamin D 3 -stimulated osteocalcin production on as-received and calcined HA surfaces could be attributed to many factors, including ingestion of HA particles and intracellular solubilization. 39 It was observed that intracellular dissolution of calciumcontaining crystals greatly influence cell behavior. [40][41][42] The presence of HA particles and its intracellular solubilization were hypothesized to adversely affect calcium and phosphate homoeostatic mechanisms and to modify the mechanical regulators of DNA synthesis without any expression of cytotoxic effect.…”

Section: Discussionmentioning

confidence: 99%

Osteoblast precursor cell activity on HA surfaces of different treatments

Ong

Hoppe

Cardenas

et al. 1998

J. Biomed. Mater. Res.

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The clinical success of dental implants is governed by implant surfaces and bone cell responses that promote rapid osseointegration and long-term stability. The specific objective of this study was to investigate osteoblast precursor cell responses to hydroxyapatite (HA) surfaces of different treatments. Since the nature of bone cell responses in vitro is influenced by the properties of HA ceramics, this study was divided into two components: a chemical and crystallographic characterization of the HA ceramics and an in vitro cell culture study. The sintered HA samples were observed to have the highest crystallite size as compared to the as-received HA and calcined HA samples. No differences in the surface roughness and chemical composition were observed among the sintered, calcined, and as-received HA surfaces. In concurrence with the X-ray diffraction, high resolution XPS resolution of Ca 2p also indicated a higher crystallinity on sintered HA samples as compared to the calcined and as-received HA samples. As indicated by increased alkaline phosphatase-specific activity, increased cell-surface and matrix-associated protein, and 1.25 (OH2) vitamin D3-stimulated osteocalcin production, a more differentiated osteoblast-like phenotype was observed on the sintered HA surfaces compared to the as-received HA and calcined HA surfaces. An increased osteoblast-like cell activity on the sintered HA surfaces suggested that the crystallite size of HA surfaces may play an important role in governing cellular response.

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Section: Discussionmentioning

confidence: 99%

Osteoblast precursor cell activity on HA surfaces of different treatments

Ong

Hoppe

Cardenas

et al. 1998

J. Biomed. Mater. Res.

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“…12 In this study, grains as small as 5-10 m in diameter were seen integrated into the newly formed bone trabeculae, corroborating previous studies that reported bone formation in the presence of HA particles of less than 15 m. 13 However, it was established that such small particles (less than 10 m) were often subjected to phagocytosis by mono-and multinucleated histiocytic cells 14,15 and even by fibroblast or osteoblast-like cells. 16 Indeed, many small CaP grains were gathered in the intertrabecular spaces and elicited activation of macrophages (MC) and recruitment of multinucleated giant cells (GMNC). However, this foreign-body cell reaction was not associated with granuloma formation and did not prevent new bone formation as nonbioactive particles do.…”

Section: Discussionmentioning

confidence: 99%

Long-term bone response to particulate injectable ceramic

Dupraz¹,

Delécrin²,

Moreau³

et al. 1998

J. Biomed. Mater. Res.

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Biphasic calcium phosphate particles (BCP), alone or combined with a cellulosic ether vehicle in an injectable composite material (COMP), were implanted in femurs of rabbits. The long-term follow-up (up to 78 weeks) indicated: (1) BCP and COMP induced a foreign-body inflammatory reaction but without fibrous encapsulation. Phagocytosis was mediated by mononucleated macrophages (MC) and giant multinucleated cells (GMNC). Phagocytosis was stronger with COMP and required the recruitment of GMNC while it primarily involved MC in the case of BCP. (2) There appeared to be no significant difference between the bone ingrowth in the defects packed with BCP (dBCP) and in those filled with COMP (dCOMP). Bone reconstruction mostly was achieved after 4 weeks in dBCP but took more time to reach the center of dCOMP. High bone remodeling was observed at the last evaluation times, especially in the case of COMP. (3) Degradation of the materials occurred mainly during the first 4 weeks and was more severe for COMP, which probably was related to the smaller granulometry of its mineral phase. Cell-mediated degradation went on for the 78 weeks and followed two processes: phagocytosis and/or extracellular dissolution of the calcium phosphate particles.

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“…Saad et al 21 described limited phagocytosis of degradation particles of polyesterurethane foam by primary rat osteoblasts and the mouse osteogenic cell line MC3T3-E1 without any signs of cellular damage or reduced ability for adhesion. In addition, phagocytosis by osteoblasts has been reported for particles of synthetic calcium phosphate, 22,23 calcified bone matrix and collagen fibrils suggesting a possible participation of osteoblasts in bone resorption. 23 We did not observe a striking change in the behaviour of the osteoblasts.…”

Section: Discussionmentioning

confidence: 99%

Human osteoblast-like cells phagocytose metal particles and express the macrophage marker CD68 in vitro

Heinemann

Lohmann²,

Siggelkow³

et al. 2000

The Journal of Bone and Joint Surgery. British volume

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P eriprosthetic osteolysis is a major cause of aseptic loosening in artificial joint replacement. It is assumed to occur in conjunction with the activation of macrophages. We have shown in vitro that human osteoblast-like cells, isolated from bone specimens obtained from patients undergoing hip replacement, phagocytose fine particles of titanium alloy (TiAlV).The human osteoblast-like cells were identified immunocytochemically by the presence of bone-specific alkaline phosphatase (BAP). With increasing duration of culture, a variable number of the osteoblastic cells became positive for the macrophage marker CD68, independent of the phagocytosis of particles, with a fine granular cytoplasmic staining which was coexpressed with BAP as revealed by immunodoublestaining. The metal particles were not toxic to the osteoblastic cells since even in culture for up to four weeks massively laden cells were vital and had a characteristic morphology. Cells of the human osteosarcoma cell line (HOS 58) were also able to phagocytose metal particles but had only a low expression of the CD68 antigen. Fluorescence-activated cell scanning confirmed our immunocytochemical results. Additionally, the cells were found to be negative for the major histocompatibility complex-II (MHC-II) which is a marker for macrophages and other antigen-presenting cells. Negative results of histochemical tests for tartrate-resistant acid phosphatase excluded the contamination by osteoclasts or macrophages in culture.Our observations suggest that the osteoblast can either change to a phagocytosing cell or that the phagocytosis is an underestimated property of the osteoblast. The detection of the CD68 antigen is insufficient to prove the monocytic lineage. In order to discriminate between macrophages and osteoblasts additional markers should be used. To our knowledge, this is the first demonstration of cells of an osteoblastic origin which have acquired a mixed phenotype of both osteoblasts and macrophages. Received 17 December 1998; Accepted after revision 21 May 1999Resorption of the surrounding bone is commonly associated with aseptic loosening of artificial joints.1-4 Particles of titanium, a common material used for endoprostheses, are released from the surfaces into the surrounding tissue by corrosion or wear. 3,4 The release of particulate wear debris is generally accepted to be responsible for the recruitment of monocytes/macrophages and the formation of foreign-body granulomas. Phagocytosis of wear particles by macrophages stimulates these cells to produce and secrete inflammatory mediators 5,6 which have been implicated in the stimulation of bone resorption. 7,8The role of osteoblasts in contact with wear particles is poorly understood. We have investigated the behaviour of cultured human osteoblast-like cells when exposed to metal particles in vitro. To exclude the presence of cells of myeloid origin, we also studied markers such as CD68, tartrate-resistant acid phosphatase (TRAP) and major histocompatibility complex II (MHC II). CD68 is used a...

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Cellular activity of osteoblasts in the presence of hydroxyapatite: an in vitro experiment

Cited by 71 publications

References 33 publications

Osteoblast precursor cell activity on HA surfaces of different treatments

Osteoblast precursor cell activity on HA surfaces of different treatments

Long-term bone response to particulate injectable ceramic

Human osteoblast-like cells phagocytose metal particles and express the macrophage marker CD68 in vitro

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