Celluclast 1.<scp>5L</scp> pretreatment enhanced aroma of palm kernels and oil after kernel roasting

Zhang, Wencan; Zhao, Fangju; Yang, Tiankui; Zhao, Feifei; Xu, Dingde

doi:10.1002/jsfa.8394

Cited by 10 publications

(7 citation statements)

References 34 publications

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“…Conditions of 55 °C seemed to be the optimum for all of the cases, either using pretreated biomass or not, which is in agreement with most Based on these results, the optimum pH found for each enzyme was used to test the temperature of the process (Figure 5). Conditions of 55 • C seemed to be the optimum for all of the cases, either using pretreated biomass or not, which is in agreement with most of the previous works on enzymatic pretreatment using Viscozyme, Celluclast, and Alcalase, where their optimum temperature ranged from 35 to 55 • C, achieving its maximum at 55 • C [27,[29][30][31][32].…”

Section: Optimization Of the Ultrasound Enzymatic Pretreatmentsupporting

confidence: 90%

“…Foods 2021, 10, x FOR PEER REVIEW 8 of 14 of the previous works on enzymatic pretreatment using Viscozyme, Celluclast, and Alcalase, where their optimum temperature ranged from 35 to 55 °C, achieving its maximum at 55 °C [27,[29][30][31][32]. Thus, the optimal conditions for the synergic effect of enzymatic pretreatment and US incubation were incubation for 6 h at pH 5.0 and 55 °C for all enzymes studied (Viscozyme, Celluclast, and Alcalase).…”

Section: Optimization Of the Ultrasound Enzymatic Pretreatmentmentioning

confidence: 99%

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Combination of Synergic Enzymes and Ultrasounds as an Effective Pretreatment Process to Break Microalgal Cell Wall and Enhance Algal Oil Extraction

Blanco-Llamero

García-García

Señoráns

2021

Foods

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Microalgal biomass is a sustainable source of bioactive lipids with omega-3 fatty acids. The efficient extraction of neutral and polar lipids from microalgae requires alternative extraction methods, frequently combined with biomass pretreatment. In this work, a combined ultrasound and enzymatic process using commercial enzymes Viscozyme, Celluclast, and Alcalase was optimized as a pretreatment method for Nannochloropsis gaditana, where the Folch method was used for lipid extraction. Significant differences were observed among the used enzymatic pretreatments, combined with ultrasound bath or probe-type sonication. To further optimize this method, ranges of temperatures (35, 45, and 55 °C) and pH (4, 5, and 8) were tested, and enzymes were combined at the best conditions. Subsequently, simultaneous use of three hydrolytic enzymes rendered oil yields of nearly 29%, showing a synergic effect. To compare enzymatic pretreatments, neutral and polar lipids distribution of Nannochloropsis was determined by HPLC–ELSD. The highest polar lipids content was achieved employing ultrasound-assisted enzymatic pretreatment (55 °C and 6 h), whereas the highest glycolipid (44.54%) and PE (2.91%) contents were achieved using Viscozyme versus other enzymes. The method was applied to other microalgae showing the potential of the optimized process as a practical alternative to produce valuable lipids for nutraceutical applications.

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Section: Optimization Of the Ultrasound Enzymatic Pretreatmentsupporting

confidence: 90%

Section: Optimization Of the Ultrasound Enzymatic Pretreatmentmentioning

confidence: 99%

Combination of Synergic Enzymes and Ultrasounds as an Effective Pretreatment Process to Break Microalgal Cell Wall and Enhance Algal Oil Extraction

Blanco-Llamero

García-García

Señoráns

2021

Foods

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“…The physical methods such as ultrasounds (USs) accelerate the action of the enzymes, showing a synergic effect of both pretreatments, enzymatic and US, on the break of N. gaditana cell wall. When each pretreatment was applied separately on the microalgae biomass the oil yield was fewer than the one obtained by their combination, obtaining an effective combined pretreatment previously optimized in other works of the research group ( Zuorro et al, 2016 ; Chen et al, 2017 ; Zhang et al, 2017 ; Zhang et al, 2018a ; Dixon and Wilken, 2018 ; Wang et al, 2019 ; Blanco-Llamero et al, 2021 ).…”

Section: Introductionmentioning

confidence: 95%

Cross-Linked Enzyme Aggregates and Their Application in Enzymatic Pretreatment of Microalgae: Comparison Between CLEAs and Combi-CLEAs

Blanco-Llamero

García-García

Señoráns

2021

Front. Bioeng. Biotechnol.

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Carrier-free immobilization is a key process to develop efficient biocatalysts able to catalyze the cell wall degradation in microalgae where the traditional solid supports cannot penetrate. Thus, the insolubilization of commercial Celluclast®, Alcalase®, and Viscozyme® enzymes by carrier-free immobilization and their application in microalgae pretreatment was investigated. In this study, different precipitants at different ratios (ethanol, acetone, and polyethylene glycol 4000) were tested in the first part of the method, to establish the precipitation conditions. The screening of the best precipitant is needed as it depends on the nature of the enzyme. The best results were studied in terms of immobilization yield, thermal stability, and residual activity and were analyzed using scanning electron microscopy. Moreover, a novel strategy was intended including the three enzymes (combi-CLEAs) to catalyze the enzymatic degradation of Nannochloropsis gaditana microalgal cell wall in one pot. The carrier-free immobilized derivatives were 10 times more stable compared to soluble enzymes under the same. At the best conditions showed its usefulness in the pretreatment of microalgae combined with ultrasounds, facilitating the cell disruption and lipid recovery. The results obtained suggested the powerful application of these robust biocatalysts with great catalytic properties on novel and sustainable biomass such as microalgae to achieve cost-effective and green process to extract valuable bioactive compounds.

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“…For example, liner board coated with palm oil from palm fruit bunch’s lignin derivatives exhibited superior water-resistant properties . Thus, we assume that PKO from the seed of palm fruit will be similar to palm oil and be consider as a potential promising candidate for edible, renewable, and biodegradable paper coating with suitable fabrication and curing process. , Additionally, in some regions where PKO is present, it is used as cooking and as microbial ingredients in lotions to prevent skin damages. , …”

Section: Introductionmentioning

confidence: 99%

“…35,36 Additionally, in some regions where PKO is present, it is used as cooking and as microbial ingredients in lotions to prevent skin damages. 37,38 In this work, a facile method has been proposed to fabricate an ecofriendly, low-cost, and water-resistant paper coating using palm kernel oil. Food grade hexane is used as an organic solvent for dissolving the PKO.…”

Section: ■ Introductionmentioning

confidence: 99%

Facile Approach for Ecofriendly, Low-Cost, and Water-Resistant Paper Coatings via Palm Kernel Oil

Zeng

Cao

2020

ACS Appl. Mater. Interfaces

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Paper-based packaging is widely employed in industries ranging from food to beverages to pharmaceuticals because of its attractive advantages of biodegradability, recyclability, good strength, low cost, and lightweight. However, paper products usually have poor water barrier resistance properties because of paper and fibers porous microstructure. In this study, an ecofriendly water-resistant (hydrophobic) oil from biological origin, namely, palm kernel oil (PKO) was used to coat paper by using a facile and cost-effective dip-casting approach. PKO formulation was prepared by mixing with a solvent and furfuryl alcohol (FA). The water resistance, structural properties, and thermal and mechanical properties of the coated papers obtained under different processing conditions were reported and compared to understand the performance of coated paper. Contact angle (CA), Fourier transform infrared (FTIR), and thermal gravimetry (TGA) were used for analysis and characterization of coated papers. Data from contact angle measurements showed that the PKO formulation could considerably improve the liquid water barrier property of the paper, with a measured water contact angle (CA) of ∼120° and reduce the water vapor transmission rate (WVTR) by 22%. This novel, green, low-cost, and water-resistant paper coating made with biological and biodegradable oil is a potential candidate for replacing petroleum-based coatings used in a broad range of applications and will also be able to make an additional full use of the palm kernel oil.

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Celluclast 1.5L pretreatment enhanced aroma of palm kernels and oil after kernel roasting

Abstract: Celluclast pretreatment of PK followed by roasting may be a promising new way of improving PKO aroma. © 2017 Society of Chemical Industry.

Cited by 10 publications

References 34 publications

Combination of Synergic Enzymes and Ultrasounds as an Effective Pretreatment Process to Break Microalgal Cell Wall and Enhance Algal Oil Extraction

Combination of Synergic Enzymes and Ultrasounds as an Effective Pretreatment Process to Break Microalgal Cell Wall and Enhance Algal Oil Extraction

Cross-Linked Enzyme Aggregates and Their Application in Enzymatic Pretreatment of Microalgae: Comparison Between CLEAs and Combi-CLEAs

Facile Approach for Ecofriendly, Low-Cost, and Water-Resistant Paper Coatings via Palm Kernel Oil

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