Cells of the Mononuclear Phagocyte Series Differentiate Into Osteoclastic Lacunar Bone Resorbing Cells

The immunophenotype of osteoclasts and macrophage polykaryons

“…Both macrophage polykaryons and osteoclasts can be formed in cultures of monocytes and macrophages 6–8 20. Our findings in this study indicate that both osteoclasts and macrophage polykaryons express CD45, CD51 and CD68, and that in formalin-fixed tissue, osteoclasts are distinguished by lack of expression of the macrophage-associated antigens CD14 and HLA-DR.…”

Enhancing Alendronate Release from a Novel PLGA/Hydroxyapatite Microspheric System for Bone Repairing Applications

“…Generally, there are three mainstream opinions about the origination of osteoclasts: 1) osteoclasts originate from the fusion of monocytes and/or macrophages; 2) monocytes, macrophages and osteoclasts all originate from common progenitor; and 3) osteoclasts are differentiated from macrophages, among which the third one is best acknowledged (38). The study by Quinn et al (39,40) confirmed that mature monocytes and macrophages are capable of differentiating into osteoclasts when co-cultured with specific bone-derived stromal cell lines in the presence of 1.25 (OH) 2 D 3 and human M-CSF. CohenSela et al (41) reported that liposome-encapsulating BPs in polymeric nanoparticles transiently depleted of monocytes and macrophages.…”

The multi-differentiation potential of peripheral blood mononuclear cells

“…OCs formed by the fusion of mononuclear precursors of the monocyte/macrophage lineage present in the peripheral circulation have been demonstrated in vitro , although there is no conclusive evidence to suggest that OCs can also be formed from the fusion of PBMCs [119,120]. It is possible, however, that PBMCs undergo nuclear division but not cytokinesis [73].…”