Cells of human breast milk

Witkowska-Zimny, Małgorzata; Kamińska-El-Hassan, Ewa

doi:10.1186/s11658-017-0042-4

Cited by 197 publications

(186 citation statements)

References 63 publications

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“…A portion of the breast milk expresses markers for mesenchymal stem cells (MSCs) [10]. Interestingly, a subpopulation of nestin-positive cells has also been reported in breast milk cells [11]. Nestin is a marker for neural stem cells.…”

Section: Introductionmentioning

confidence: 99%

The Therapeutic Potential of Conditioned Medium from Human Breast Milk Stem Cells in Treating Spinal Cord Injury

2020

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Study Design: Experimental animal study.Purpose: This study investigated the therapeutic effects of human breast milk stem cell (BMSC)-conditioned medium (BMSC-CM) in a model of spinal cord injury (SCI) in male Sprague-Dawley rats.Overview of Literature: SCI is one of the leading causes of disability in addition to sensory and motor impairment. So far, there have been no successful treatments for SCI. Given the positive outcomes associated with using stem cells and their derivatives as a treatment for various diseases, there is a growing interest in using them as an SCI treatment. Recent research has demonstrated that CM from stem cells has therapeutic advantages.Methods: Human BMSCs were isolated and characterized, and CM was subsequently collected. Animals received an intrathecal administration of BMSC-CM after SCI. The activity of caspase-3 was measured to assess apoptosis, and levels of tumor necrosis factor-α and interleukin-1β were measured to assess inflammation. Also, sensory and locomotor performances were assessed after SCI and BMSC-CM administration.Results: Administration of CM from BMSC reduced apoptosis and inflammation at the site of injury in a rat model of SCI (p<0.05). Motor, sensory, locomotor, and sensorimotor performances were significantly improved in rats that received BMSC-CM after SCI.Conclusions: Intrathecal administration of BMSC-CM improved recovery in a rat model of SCI.

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Section: Introductionmentioning

confidence: 99%

The Therapeutic Potential of Conditioned Medium from Human Breast Milk Stem Cells in Treating Spinal Cord Injury

2020

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“…Some cells such as MECs, macrophages, neutrophils, lymphocytes and stem cells are also found in milk [79,80].…”

Section: Milk Compositionmentioning

confidence: 99%

Physiology of milk secretion

Truchet

Honvo-Houeto

2017

Best Practice & Research Clinical Endocrinology & Metab

100

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“…Thus, it was unclear if we could initiate a robust lamprey immune response with soluble sugars. We approached this question by immunizing with unmodified human milk, which is a complex mixture of lipids, soluble proteins, many unique free glycans and maternal epithelial cells, leukocytes and beneficial bacteria 38 . Lamprey were immunized five times with 20 µl of whole milk and the resulting plasma, contained VLRBs bound to previously described free human milk oligosaccharides (Fig.…”

Section: Resultsmentioning

confidence: 99%

Development of smart anti-glycan reagents using immunized lampreys

et al. 2020

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Studies on the expression of cellular glycans are limited by a lack of sensitive tools that can discriminate specific structural features. Here we describe the development of a robust platform using immunized lampreys (Petromyzon marinus), which secrete variable lymphocyte receptors called VLRBs as antibodies, for generating libraries of anti-glycan reagents. We identified a wide variety of glycan-specific VLRBs detectable in lamprey plasma after immunization with whole fixed cells, tissue homogenates, and human milk. The cDNAs from lamprey lymphocytes were cloned into yeast surface display (YSD) libraries for enrichment by multiple methods. We generated VLRB-Ig chimeras, termed smart anti-glycan reagents (SAGRs), whose specificities were defined by microarray analysis and immunohistochemistry. 15 VLRB antibodies were discovered that discriminated between linkages, functional groups and unique presentations of the terminal glycan motif. The development of SAGRs will enhance future studies on glycan expression by providing sequenced, defined antibodies for a variety of research applications.

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Cells of human breast milk

Cited by 197 publications

References 63 publications

The Therapeutic Potential of Conditioned Medium from Human Breast Milk Stem Cells in Treating Spinal Cord Injury

The Therapeutic Potential of Conditioned Medium from Human Breast Milk Stem Cells in Treating Spinal Cord Injury

Physiology of milk secretion

Development of smart anti-glycan reagents using immunized lampreys

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