Cells of Epithelial Lineage Are Present in Blood, Engraft the Bronchial Epithelium, and Are Increased in Human Lung Transplantation

May, L.A.; Kicic, Anthony; Rigby, Paul; Heel, Kathy; Lee-Pullen, Tracey F.; Crook, Maxine L.; Charles, Adrian; Banerjee, Balarka; Ravine, David; Saxena, Alka; Musk, M.; Stick, Stephen M.; Chambers, Daniel C.

doi:10.1016/j.healun.2009.03.013

Cited by 7 publications

(7 citation statements)

References 26 publications

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“…The Th1 response, with increased expression of interferon-γ, interleukin (IL)-2, IL-12, and IL-18, may play a role helping tissue repair, whereas the Th2 cytokines, including IL-4, IL-5, IL-10, and IL-13, tend to promote fibroproliferation. 48 Certain chemokine receptors expressed on lung epithelial cells, such as CXCR3 [49][50][51] for Th1 responses, and CCR4, 52 CCR8, 53 and CXCR4 [54][55][56] for Th2 responses, can modulate the transition from lung restoration and repair to progressive lung fibrosis.…”

Section: Biochemical Insultsmentioning

confidence: 99%

Mechanical Ventilation–associated Lung Fibrosis in Acute Respiratory Distress Syndrome

et al. 2014

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One of the most challenging problems in critical care medicine is the management of patients with the acute respiratory distress syndrome. Increasing evidence from experimental and clinical studies suggests that mechanical ventilation, which is necessary for life support in patients with acute respiratory distress syndrome, can cause lung fibrosis, which may significantly contribute to morbidity and mortality. The role of mechanical stress as an inciting factor for lung fibrosis versus its role in lung homeostasis and the restoration of normal pulmonary parenchymal architecture is poorly understood. In this review, the authors explore recent advances in the field of pulmonary fibrosis in the context of acute respiratory distress syndrome, concentrating on its relevance to the practice of mechanical ventilation, as commonly applied by anesthetists and intensivists. The authors focus the discussion on the thesis that mechanical ventilation-or more specifically, that ventilator-induced lung injury-may be a major contributor to lung fibrosis. The authors critically appraise possible mechanisms underlying the mechanical stress-induced lung fibrosis and highlight potential therapeutic strategies to mitigate this fibrosis.

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Section: Biochemical Insultsmentioning

confidence: 99%

Mechanical Ventilation–associated Lung Fibrosis in Acute Respiratory Distress Syndrome

et al. 2014

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“…On the other hand, concerning the mechanism(s) behind the phenomenon, it could be that cytokines are mimicking signals coming from the damaged lung. It has been proposed that chemokines, such as SDF-1 (or CXCL12), are the main stimuli which attract circulating CXCR4-expressing epithelial progenitor cells to the lung in rodents and humans [56, 136, 137]. …”

Section: Heterogeneity Of Marrow-derived Stem/progenitor Cellsmentioning

confidence: 99%

Hematopoietic and Mesenchymal Stem Cells for the Treatment of Chronic Respiratory Diseases: Role of Plasticity and Heterogeneity

Conese

Piro

Carbone

et al. 2014

The Scientific World Journal

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Chronic lung diseases, such as cystic fibrosis (CF), asthma, and chronic obstructive pulmonary disease (COPD) are incurable and represent a very high social burden. Stem cell-based treatment may represent a hope for the cure of these diseases. In this paper, we revise the overall knowledge about the plasticity and engraftment of exogenous marrow-derived stem cells into the lung, as well as their usefulness in lung repair and therapy of chronic lung diseases. The lung is easily accessible and the pathophysiology of these diseases is characterized by injury, inflammation, and eventually by remodeling of the airways. Bone marrow-derived stem cells, including hematopoietic stem/progenitor cells (HSPCs) and mesenchymal stromal (stem) cells (MSCs), encompass a wide array of cell subsets with different capacities of engraftment and injured tissue regenerating potential. Proof-of-principle that marrow cells administered locally may engraft and give rise to specialized epithelial cells has been given, but the efficiency of this conversion is too limited to give a therapeutic effect. Besides the identification of plasticity mechanisms, the characterization/isolation of the stem cell subpopulations represents a major challenge to improving the efficacy of transplantation protocols used in regenerative medicine for lung diseases.

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“…Since CD45 and CD11b proteins were reported to be present on lung and airway progenitor cells as well as on monocytic and hematopoietic subsets, we set out to determine the abundance of these markers in the AIC population (Rajantie et al ., ; May et al ., ). We found predominant expression of CD45 + and CD11b + surface receptors within the AIC population, with CD45 + /CD11b + double‐positive cells constituting ~ 60% of this fraction (Figures A‐D).…”

Section: Resultsmentioning

confidence: 97%

“…Assuming a hematopoietic cell phenotype, the significant presence of CD45 and CD11b expressing cells in the AIC population might suggest that: 1) the lung can function as a satellite organ of hematopoiesis to promote blood regeneration; 2) blood cells can support lung progenitor cells outside the niche and/or; 3) they can serve as a source of pulmonary cell precursors. These possibilities correspond with reports that identified CD45 and CD45 + /CD11b + expressing cells as epithelial and endothelial progenitors of the lung that participate in postnatal vascular and airway repair and regeneration (Rajantie et al ., ; Yamada and Takakura, ; Zani et al ., ; May et al ., ).…”

Section: Resultsmentioning

confidence: 97%

“…Our findings are consistent with prior reports that suggest that c‐KIT is a marker for lung multipotential stem cells and confirm the existence of fibroblastic and epithelial lineages within cells of the AIC population (Horwitz and Dorfman, ; Shin et al ., ; Torry et al ., ; Weinberg, ; May et al ., ; Kajstura et al ., ). Furthermore, AIC proliferative growth recapitulates that of pluripotential colony‐forming bone marrow and embryonic stem cells grown in suspension (Iscove and Yan, ; zur Nieden et al ., ), and conveys plasticity when subjected to diverse environmental conditions or chemokines (Willis et al ., ).…”

Section: Resultsmentioning

confidence: 99%

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CD45/CD11b positive subsets of adult lung anchorage-independent cells harness epithelial stem cells in culture

Peter

Sen

Keller

et al. 2012

J Tissue Eng Regen Med

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Compensatory growth is mediated by multiple cell types that interact during organ repair. To elucidate the relationship between the stem/progenitor cells that proliferate or differentiate and the somatic cells of lung, we utilized a novel ex vivo pneumoexplant system. Applying this technique, we identified a sustained culture of repopulating adult progenitors in the form of free floating anchorage-independent cells (AICs). AICs did not express integrin proteins α5, β3, and β7, and constituted 37% of the total culture at day 14, yielding a mixed yet conserved population that recapitulated RNA expression patterns of the healthy lung. AICs exhibited rapid proliferation manifested by a marked 60-fold increase in cell numbers by day 21. Over 50% of the AIC population was cKit+ or double-positive for CD45+ and CD11b+ antigenic determinants, consistent with cells of hematopoietic origin. The latter subset was found to be enriched with prosurfactant protein-C and SCGB1A1 expressing putative stem cells and with aquaporin-5 producing cells, characteristic of terminally differentiated alveolar epithelial type-1 pneumocytes. AICs undergo remodeling to form a cellular lining at the air/gel interface, and TGFβ1 treatment modifies protein expression, implying direct-differentiation of this population. These data confirm the active participation of clonogenic hematopietic stem cells in a mammalian model of lung repair and validate mixed stem/somatic cell cultures, which embrace sustained cell viability, proliferation, and differentiation, for use in studies of compensatory pulmonary growth.

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Cells of Epithelial Lineage Are Present in Blood, Engraft the Bronchial Epithelium, and Are Increased in Human Lung Transplantation

Cited by 7 publications

References 26 publications

Mechanical Ventilation–associated Lung Fibrosis in Acute Respiratory Distress Syndrome

Mechanical Ventilation–associated Lung Fibrosis in Acute Respiratory Distress Syndrome

Hematopoietic and Mesenchymal Stem Cells for the Treatment of Chronic Respiratory Diseases: Role of Plasticity and Heterogeneity

CD45/CD11b positive subsets of adult lung anchorage-independent cells harness epithelial stem cells in culture

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