Cells Isolated from Adult Human Skeletal Muscle Capable of Differentiating into Multiple Mesodermal Phenotypes

Williams, John T.; Southerland, Shiela S.; Souza, John W.; Calcutt, Andrew F.; Cartledge, Richard G.

doi:10.1177/000313489906500106

Cited by 193 publications

(10 citation statements)

References 6 publications

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“…A valid alternative to osteoblasts is represented by mesenchymal stem cells (MSCs), also known as skeletal stem cells or bone marrow stromal cells [13]. It has been proved that these progenitor cells can differentiate into osteogenic, chondrogenic and adipogenic lineages [14] and preserve this differentiation potential after in vitro expansion [15]. Bone marrow, periosteum, adipose tissue, umbilical cord and placenta are considered possible human MSC sources [16].…”

Section: Introductionmentioning

confidence: 99%

Toward mimicking the bone structure: design of novel hierarchical scaffolds with a tailored radial porosity gradient

Luca¹,

Longoni²,

Criscenti³

et al. 2016

Biofabrication

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Guiding bone regeneration poses still unmet challenges due to several drawbacks of current standard treatments in the clinics. A possible solution may rely on the use of three-dimensional scaffolds with optimized structural properties in combination with human mesenchymal stem cells (hMSCs). Bone presents a radial gradient structure from the outside, where the cortical bone is more compact (porosity ranging from 5% to 10%), toward the inner part, where the cancellous bone is more porous (porosity ranging from 50% to 90%). Here, we present a new scaffold design with a built-in gradient in porosity, which approximate the radial bone structure. The pores of the outer ring were 500 μm, the ones in the middle zone were 750 μm and the inner part presented pores of 1000 μm. The porosity of each scaffold region resembled the gradient present in bone, with the outer ring having a porosity of 29.6% ± 5%, the middle and inner regions a porosity of 50.8% ± 8.1% and 77.6% ± 3.2% respectively. hMSCs behavior was analyzed in terms of growth, extracellular matrix deposition and differentiation toward the osteogenic lineage. A trend was displayed by the hMSCs residing in different zones of the gradient scaffolds after 7, 14 and 28 days of culture in mineralization medium. Osteogenic differentiation was influenced by pore size and location in scaffolds displaying a radial porosity gradient. Cell differentiation was confirmed by gene expression with upregulation of Runx2 and bone sialoprotein markers. Mineralization staining further confirmed the maturation of cell differentiation, as indicated by the presence of calcium and phosphate mineral deposits.

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Section: Introductionmentioning

confidence: 99%

Toward mimicking the bone structure: design of novel hierarchical scaffolds with a tailored radial porosity gradient

Luca¹,

Longoni²,

Criscenti³

et al. 2016

Biofabrication

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show abstract

“…Mesenchymal stem cells have recently attracted much interest for possible clinical use because of their self-renewing potential and multipotency. Thus far, MSCs have been identified in a variety of tissues, including the bone marrow, muscle, periosteum, neural tissue, and adipose tissue [38][39][40][41][42][43]. Among these tissues, the bone marrow has been well established as an MSC source in humans.…”

Section: Discussionmentioning

confidence: 99%

Deer antler reserve mesenchyme cells with hyaluronan alleviates cartilage damage in a rat model

Jia,

Li,

Han

et al. 2023

Animal Research and One Health

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Reserve mesenchyme cells (RMCs) of deer antlers have been considered as the promising cell source for repairing injury‐induced articular cartilage or cartilage degeneration. However, systematic investigation of RMC differentiation to repair injured cartilage and its combination with biomaterials has not been reported. The aim of this study was to evaluate the role of RMCs in combination with hyaluronic acid (HA) in promoting chondrogenic differentiation through simulating native environments and their efficacy in articular cartilage repair. The RMCs were cultured in vitro for the characterization of these cells, including morphology, surface marker expression, and multipotent differentiation potential (adipogenesis, chondrogenesis, and osteogenesis). When combined with HA in vitro, RMCs increased expression levels of the chondrogenic marker gene (COL II and COMP) but decreased levels of the hypertrophic marker gene (COL X). Using a rat articular cartilage defect model, we evaluated the effects of RMCs in combination with HA on cartilage defect repair at 4 and 8 weeks through macroscopical, histological, and immunohistochemical examinations. Compared with other groups, treatment with RMCs + HA reduced cartilage loss and degree of cartilage surface worn, whereas cartilage content was significantly increased. These results suggest that the combination of RMCs with HA can effectively repair cartilage defects. We believe that effective cartilage defect repair will benefit from the use of RMCs together with favorable biomaterials, such as HA.

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“…The MSCs can differentiate into osteogenic, chondrogenic, and adipogenic cell lineage according to these criteria ( Dominici et al, 2006 ). It is well established that MSCs can be found in almost all post-natal tissues and may be obtained from various tissues such as skeletal muscle ( Williams et al, 1999 ), BM ( Haynesworth et al, 1992 ), umbilical cord ( Erices et al, 2000 ), placenta ( In’t Anker et al, 2004 ), and adipocyte ( Halvorsen et al, 2000 ; Go et al, 2020 ). The first isolated human dental MSCs were derived from pulp tissue ( Gronthos et al, 2000 ).…”

Section: Cartilage Regenerationmentioning

confidence: 99%

Hopes and opportunities of stem cells from human exfoliated deciduous teeth (SHED) in cartilage tissue regeneration

Mahdavi-Jouibari

Parseh

Kazeminejad

et al. 2023

Front. Bioeng. Biotechnol.

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Cartilage lesions are common conditions, affecting elderly and non-athletic populations. Despite recent advances, cartilage regeneration remains a major challenge today. The absence of an inflammatory response following damage and the inability of stem cells to penetrate into the healing site due to the absence of blood and lymph vessels are assumed to hinder joint repair. Stem cell-based regeneration and tissue engineering have opened new horizons for treatment. With advances in biological sciences, especially stem cell research, the function of various growth factors in the regulation of cell proliferation and differentiation has been established. Mesenchymal stem cells (MSCs) isolated from different tissues have been shown to increase into therapeutically relevant cell numbers and differentiate into mature chondrocytes. As MSCs can differentiate and become engrafted inside the host, they are considered suitable candidates for cartilage regeneration. Stem cells from human exfoliated deciduous teeth (SHED) provide a novel and non-invasive source of MSCs. Due to their simple isolation, chondrogenic differentiation potential, and minimal immunogenicity, they can be an interesting option for cartilage regeneration. Recent studies have reported that SHED-derived secretome contains biomolecules and compounds that efficiently promote regeneration in damaged tissues, including cartilage. Overall, this review highlighted the advances and challenges of cartilage regeneration using stem cell-based therapies by focusing on SHED.

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Cells Isolated from Adult Human Skeletal Muscle Capable of Differentiating into Multiple Mesodermal Phenotypes

Cited by 193 publications

References 6 publications

Toward mimicking the bone structure: design of novel hierarchical scaffolds with a tailored radial porosity gradient

Toward mimicking the bone structure: design of novel hierarchical scaffolds with a tailored radial porosity gradient

Deer antler reserve mesenchyme cells with hyaluronan alleviates cartilage damage in a rat model

Hopes and opportunities of stem cells from human exfoliated deciduous teeth (SHED) in cartilage tissue regeneration

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