Cells in the intestinal system of holoturians (echinodermata) express cholecystokinin-like immunoreactivity

García-Arrarás, JoséE.; Torres-Avillán, Ilia; Ortiz‐Miranda, Sonia

doi:10.1016/0016-6480(91)90026-3

Cited by 27 publications

(18 citation statements)

References 14 publications

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“…There is increasing evidence for the existence of various neuropeptides in echinoderms (Shirai and Walker 1988;Elphick et al 1991;Díaz-Miranda et al 1992), suggesting that these substances are involved in neurotransmission in these organisms. However, only in the case of GFS and to a lesser extent for CCK-like immunoreactivity is there evidence for a specific action in the putative target cell, both peptides causing relaxation of the intestine and, in the case of GFS, also relaxation of the muscle components (García-Arrarás et al 1991;. In vertebrates, galanin has been found to affect the contractile activity of the gut; however, its effects have been shown to vary across species and to depend on the area of the gut tested (Ekblad et al 1985;Fox et al 1988;Karila et al 1993).…”

Section: Possible Roles Of the Galanin-like Moleculementioning

confidence: 94%

“…The spatial expression pattern of the GAL-LI in the holothurians merits comparison to those of, CCK and GFS, two other peptides that have been shown to be expressed by cells in the holothurian nervous system (García-Arrarás et al 1991;. All are expressed by isolated nerve cells within the serosa of the intestine by fibers innervating the enteric musculature, and by cells and an extensive system of fibers within the radial nerve cord.…”

Section: Distribution Of Gal-limentioning

confidence: 98%

“…For the production of antibodies against galanin we followed a similar procedure to that previously used in the preparation of antibodies to other neuroactive substances (García-Arrarás et al 1991. In brief, 0.5 mg galanin (Calbiochem Corp., San Diego, Calif., USA) was coupled to 7.5 mg bovine serum albumin (Sigma, St. Louis, Mo., USA) using glutaraldehyde.…”

Section: Antibodiesmentioning

confidence: 99%

“…Procedures for immunohistochemistry have been described previously (García-Arrarás et al 1991. Controls were done by omitting the primary antibody or by incubating the primary antibody with 1-10 µg/ml (0.3-3 µM) galanin overnight, before applying it to the tissue sections.…”

Section: Immunohistochemistrymentioning

confidence: 99%

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Galanin-like immunoreactivity in the sea cucumber Holothuria glaberrima

Díaz‐Miranda

Pardo-Reoyo

Martínez

et al. 1996

Cell and Tissue Research

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Galanin, a neuropeptide of 29 amino acids originally purified from porcine small intestine, has been found in most vertebrate groups, where it is present in both the central and peripheral nervous systems. In this study, galanin-like immunoreactivity was detected in an invertebrate, the sea cucumber Holothuria glaberrima. In this organism, fibers and cells expressing galanin-like immunoreactivity were found in the enteric nerve plexuses of the esophagus, and large and small intestine, particularly in the serosal layer. Immunoreactivity was also found in the ectoneural portion of the radial nerve and in nervous elements within the body wall. Preabsorbtion tests indicated that the observed immunoreactivity was not due to cross-reactivity of the antibody with other peptides also found in the intestinal plexuses. The immunoreactivity to galanin was quantified in various tissues using radioimmunoassay and partially characterized with high performance liquid chromatography. These results show that although the holothurian peptide shows common immunological determinants with porcine galanin, it differs with regard to certain biochemical properties.

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Section: Possible Roles Of the Galanin-like Moleculementioning

confidence: 94%

Section: Distribution Of Gal-limentioning

confidence: 98%

Section: Antibodiesmentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

See 2 more Smart Citations

Galanin-like immunoreactivity in the sea cucumber Holothuria glaberrima

Díaz‐Miranda

Pardo-Reoyo

Martínez

et al. 1996

Cell and Tissue Research

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“…In the past few years numerous regulatory peptides have been described in the nervous system of echinoderms (Welsch et al 1989;Elphick et al 1989Elphick et al , 1991García-Arrarás et al 1991;Martínez et al 1993;Newman and Thorndyke 1994). Adrenomedullin (AM) is a potent hypotensive peptide recently isolated from human pheochromocytoma (Kitamura et al 1993).…”

Section: Introductionmentioning

confidence: 99%

Adrenomedullin-like immunoreactivity in the nervous system of the starfish Marthasterias glacialis

Martı́nez

Unsworth

Cuttitta

1996

Cell and Tissue Research

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The nervous system of the starfish Marthasterias glacialis was investigated immunocytochemically using an antiserum specific for adrenomedullin (AM), a new regulatory peptide. Immunoreactivity was only found in nerves of the basiepithelial plexus of cardiac and pyloric stomachs and pyloric caeca, while the radial nerve cords and the other digestive organs were negative. The strongest AM-like immunoreactivity was located in the current-producing areas of the cardiac stomach. The distribution of this peptide suggests different functions in echinoderms involving regulation of muscle movement and neurotransmission. The presence of an AM-like substance in echinoderms points to an early phylogenetic origin for this regulatory system.

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Heterogeneity of cholecystokinin/gastrin-like immunoreactivity in the nervous system of the nematodeAscaris suum

1996

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A wholemount immunocytochemical method was used for the localization of cholecystokinin (CCK8)-like and gastrin-like immunoreactivity in Ascaris. The patterns of specific neuronal staining given by two antisera and four monoclonal antibodies made against CCK8, and one antiserum made against gastrin were investigated. Preabsorption of these antibodies with CCK8 or gastrin 17 resulted in complete loss of immunoreactivity in almost all of the neurons (two antisera also contained nonspecific antibodies), suggesting that all of the antibodies recognize epitopes, in Ascaris neurons, that include some or all of the C-terminal five amino acids that are identical in CCK8 and gastrin 17. However, the seven different antibodies showed immunoreactivity in different subpopulations of neurons, implying that there are at least seven different species of CCK-like molecules in Ascaris. Fractionation of Ascaris peptide extracts by high performance liquid chromatography (HPLC), monitoring fractions with a CCK8 radioimmunoassay (RIA), also shows heterogeneity of molecules immunologically related to CCK8.

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Cells in the intestinal system of holoturians (echinodermata) express cholecystokinin-like immunoreactivity

Cited by 27 publications

References 14 publications

Galanin-like immunoreactivity in the sea cucumber Holothuria glaberrima

Galanin-like immunoreactivity in the sea cucumber Holothuria glaberrima

Adrenomedullin-like immunoreactivity in the nervous system of the starfish Marthasterias glacialis

Heterogeneity of cholecystokinin/gastrin-like immunoreactivity in the nervous system of the nematodeAscaris suum

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