Cells grown on isolated intravascular dacron hub

Oneal, Robert M.; Jordan, George L.; Rabin, Erwin R.; Bakey, Michael E. De; Halpert, Béla

doi:10.1016/0014-4800(64)90022-x

Cited by 64 publications

(14 citation statements)

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“…Thus none of the wellfunctioning and long-surviving grafts showed repopulation, whereas in the kidneys in which this had occurred renal function was poor and damage due to rejection was extremely severe. These findings may help to resolve the apparent contradictions of previously published work which both discounts (Alexandre et al, 1963;Murray et al, 1964;Bieber et al, 1970;Bohle and Fischbach, 1970) and supports (Stump et al, 1963;O'Neal et al, 1964;Williams and Alvarez, 1969;Williams et al, 1971) the possibility of endothelial repopulation.…”

Section: Discussionmentioning

confidence: 55%

“…The formation of a new endothelial layer from host cells occurs in aortic grafts (Williams and Alvarez, 1969;Williams et al, 1971) and on the surface of Dacron hubs suspended in the aortic blood stream (Stump et al, 1963;O'Neal et al, 1964). The recipients of the aortic grafts did not receive immunosuppressive therapy, and rejection was sufficiently violent to result in several cases of aortic rupture, aneurysm formation, and aortic occlusion.…”

Section: Discussionmentioning

confidence: 99%

“…Medawar (1965) urged investigation of the possibility that adaptation in organ grafts might be explained by an extensive replacement of the donor vascular endothelium by cells derived from the host, and much conflicting evidence has appeared (Alexandre et al, 1963;Stump et al, 1963;O'Neal et al, 1964;Murray et al, 1964;Williams and Alvarez, 1969;Bieber et al, 1970;Bohle and Fischbach, 1970;Williams et al, 1971).…”

Section: Introductionmentioning

confidence: 99%

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Origin of Endothelium in Human Renal Allografts

Sinclair¹

1972

BMJ

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SummarySex chromatin counts performed on the endothelial cells of 40 human kidneys transplanted to recipients of the opposite sex showed that the donor endothelium had persisted except in three poorly functioning and severely damaged grafts. In these a high proportion of the endothelial cells in peritubular capillaries and veins were derived from the host. Endothelial repopulation of organ allografts probably occurs only after severe tissue injury, and it cannot explain the phenomenon of graft adaptation. Repopulated endothelium may be derived from circulating cells.records of the sex of the donor and the recipient. The donor sex was readily confirmed by an examination of arterial smooth muscle cells, where sexual differentiation is very obvious.Most of the sections were examined without previous knowledge of the sex of the donor or the recipient. Between 300 and 1,000 endothelial cells were counted from the arteries, peritubular capillaries, vasa recta, and veins from each specimen. Sex chromatin body counts on glomerular endothelium were attempted in only three cases of special interest, for, though they can be seen in normal female glomeruli, nuclear irregularity is very common and confusion with epithelial and mesangial cells is bound to make the counts unreliable. Control values for the frequency of sex chromatin bodies were established in the sexmatched grafts and from earlier results (Sinclair, 1972) in six normal kidneys.

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Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Origin of Endothelium in Human Renal Allografts

Sinclair¹

1972

BMJ

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“…Since the 1960s, various groups have investigated the origins of ECs and SMCs in vascular pathologic lesions [28][29][30][31]. Medawar [32] hypothesized that allograft adaptation may actually be the result of a gradual replacement of the ECs of the donor by those of the recipient.…”

Section: Source Of Intimal Cells In Graft Arterial Diseasementioning

confidence: 99%

Stem cell origins of intimal cells in graft arterial disease

Shimizu

Mitchell

2003

Current Atherosclerosis Reports

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Long-term solid organ allografts develop diffuse arterial intimal lesions (graft arterial disease ), consisting of smooth muscle cells (SMCs), extracellular matrix, and admixed mononuclear leukocytes. Although the exact mechanisms are unknown, alloresponses likely induce inflammatory cells and/or dysfunctional vascular wall cells to secrete growth factors that promote SMC intimal recruitment, proliferation, and matrix synthesis. GAD eventually culminates in vascular stenosis and ischemic graft failure. Although prior work demonstrated that the endothelium and medial SMCs and the vast majority of endothelial cells (ECs) lining GAD lesions in cardiac allografts are derived from donors, the intimal SMC origin could not be determined. Recent reports suggest that intimal lesions in allograft vessels may also contain host-derived ECs and SMCs. In light of these findings, it is noteworthy that subpopulations of bone marrow and circulating cells have also been shown to differentiate into ECs and SMCs. Here we review recent developments in the understanding of vascular wall cell recruitment that are forcing a re-evaluation of the pathogenic mechanisms underlying GAD, as well as those occurring in more "conventional" atherosclerosis. The demonstration of the host origin of intimal SMCs in GAD lays the groundwork for future interventions where therapeutic genes or drugs may be targeted not to donor medial SMCs, but rather to recipient SM precursor cells.

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“…Some authors have suggested that endothelial cells circulating in the bloodstream, seeding on the graft surface, may contribute to the process of endothelialization (Harker et al, 1977;Mackenzie et al, 1968;O'Neal et al, 1964;Stump et al, 1963). Ts'ao suggested that endothelial cells originate from smooth muscle cells (Ts'ao, 1968).…”

Section: Downloaded By [University Of Lethbridge] At 21:38 17 June 2016mentioning

confidence: 97%

Polyurethane Vascular Grafts with Thorough Porosity: Does an Internal or an External Membrane Wrapping Improve their In Vivo Blood Compatibility and Biofunctionality?

Wang¹,

Liu²,

Guidoin³

et al. 2004

Artif Cells Blood Substit Biotechnol

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ORDER REPRINTS SUMMARYUsing a freeze-drying multiple-step technique, porous polyurethane vascular grafts 1.5 mm in diameter with different internal and external surface characteristics were fabricated: Type A: with thorough porosity, Type B: with internal membrane wrapping and Type C: with external membrane wrapping. One centimeter long tubes of each three types of grafts were implanted into the infra-renal abdominal aorta of rats for periods of 2, 4, 8 weeks. All the grafts were patent at harvesting. At the end of 2 weeks postimplantation, the endothelial cells layer developed inside the grafts about 1.2 mm apart from either the distal anastomosis side or both the distal and proximal anastomoses. They spreaded to the middle part of the one cm-long graft at 4 weeks. The luminal surfaces were entirely covered by a layer of endothelial cells at 8 weeks. Smooth muscle cells also migrated underneath the endothelial cell layer through the inner surface from the anastomosis and grew into the porous wall to generate a biocomposite blood conduit. Type B grafts with an internal membrane wrapping, developed a thicker, easy to lift intima after all the durations of implantation because the internal capsule was poorly anchored to the device. It is therefore of paramount importance that a porous structure is exposed to the blood flow to permit an adequate healing together with a good anchorage of the internal capsule.

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Cells grown on isolated intravascular dacron hub

Cited by 64 publications

References 1 publication

Origin of Endothelium in Human Renal Allografts

Origin of Endothelium in Human Renal Allografts

Stem cell origins of intimal cells in graft arterial disease

Polyurethane Vascular Grafts with Thorough Porosity: Does an Internal or an External Membrane Wrapping Improve their In Vivo Blood Compatibility and Biofunctionality?

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