Cells Derived from Human Umbilical Cord Blood Support the Long-Term Growth of Undifferentiated Human Embryonic Stem Cells

Zhan, Xi; Hill, Christine; Brayton, Cory; Shamblott, Michael J.

doi:10.1089/clo.2007.0087

Cited by 9 publications

(9 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar results were obtained by Lee et al (33), who reported that Oct3/4 was expressed in hESCs grown over placental cells. Furthermore, hESCs grown over hHFDC expressed the surface protein SSEA-4, the transcription factor Oct3/4, and alkaline phosphatase, in accordance with results reported by Zhan et al (32) and Cho et al (40), who used umbilical cord cells as feeder layers. It should be noted that none of the feeder-layer cells expressed these proteins.…”

Section: Discussionsupporting

confidence: 89%

See 1 more Smart Citation

Hair follicle-derived mesenchymal cells support undifferentiated growth of embryonic stem cells

Oliveira

Santos

Vairo

et al. 2017

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

The aim of the present study was to investigate whether feeder layers composed of human hair follicle-derived mesenchymal stem cells (hHFDCs) are able to support human embryonic stem cells (hESCs). hHFDCs and mouse embryonic fibroblasts (MEFs) were isolated and cultured in Dulbecco's modified Eagle's medium (DMEM)/F-12 and low-glucose DMEM, respectively. hHFDCs were passaged three times and subsequently characterized. hHFDCs and MEFs were mitotically inactivated with mitomycin C for 3 h prior to co-culture with H9-hESCs. hESCs were initially established on a mouse feeder layer, subsequently transferred onto a human feeder layer and split every 5 days. Cell morphology, expression of specific ‘undifferentiation’ markers and growth factors, and the differentiation capacity of hESCs grown on the hHFDC feeder layer were analyzed. hHFDCs are adherent to plastic, possess the classic mesenchymal stem cell phenotype [they express cluster of differentiation (CD)90, CD73 and CD105] and are able to differentiate into adipocytes, chondroblasts and osteocytes, indicating that these cells are multipotent. Population-doubling time analysis revealed that hHFDCs rapidly proliferate over 34.5 h. As a feeder layer, hHFDC behaved similarly to MEF in maintaining the morphology of hESCs. The results of alkaline phosphatase activity, reverse transcription-quantitative polymerase chain reaction analysis of the expression of pluripotency transcription factors [octamer-binding transcription factor 4 (Oct4), Nanog and sex determining region Y-box 2], and immunofluorescence assays of markers (stage-specific embryonic antigen-4 and Oct4) in hESCs co-cultured over hHFDC, indicated that the undifferentiated state of hESCs was preserved. No change in the level of growth factor transcripts (bone morphogenetic protein 4, fibroblast growth factor-2, vascular endothelial growth factor, Pigment epithelium-derived factor and transforming growth factor-β1) was detected for either feeder layer prior to or following inactivation. Similar phenotypes of embryoid body formation, size and morphology were observed in the hHFDC and MEF feeders. In conclusion, hHFDC maintained hESCs in an undifferentiated state comparable to MEF in standard conditions, which may be an important finding regarding the establishment of stem cell-based translational applications.

show abstract

Section: Discussionsupporting

confidence: 89%

“…Many authors use enzymatic digestion for hESCs cultured on human feeder layers (30,32–34). However, the commonly used enzymes including trypsin, collagenase type IV and dispase (35), are animal derived.…”

Section: Discussionmentioning

confidence: 99%

Hair follicle-derived mesenchymal cells support undifferentiated growth of embryonic stem cells

Oliveira

Santos

Vairo

et al. 2017

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

show abstract

“…Cells grown in this manner are not ideal for transplantation into humans due to the risk of xenogenic-based rejection by the immune system [18] as well as the potential for cross-species transfer of viruses or pathogens [19]. MEF feeder layers can be replaced with human-derived cell lines to avoid hESC contact with murine cells, but the presence of additional cell lines in hESC culture inevitably contributes to variability and increased cost during the expansion and scale-up of stem cells [19][20][21]. As a result, direct coculture has been replaced by medium conditioning, where hESC are grown on an acellular support matrix in the medium that has been conditioned by a separate, supporting cell line [19,20,22,23].…”

Section: Introductionmentioning

confidence: 99%

Proliferation and Pluripotency of Human Embryonic Stem Cells Maintained on Type I Collagen

Jones

Chu

Pendleton

et al. 2010

Stem Cells and Development

Self Cite

View full text Add to dashboard Cite

Human embryonic stem cells (hESC) require a balance of growth factors and signaling molecules to proliferate and retain pluripotency. Conditioned medium (CM) from a human embryonic germ-cell-derived cell culture, SDEC, was observed to support the growth of hESC on type I collagen (COL I) and on Matrigel (MAT) biomatricies. After 1 month, the population doubling of hESC grown in SDEC CM on COL I was equivalent to that of hESC grown in mouse embryonic fibroblast (MEF) CM on MAT. hESC grown in SDEC CM on COL I expressed OCT4, NANOG, SSEA-4, alkaline phosphatase (AP), and TRA-1-60; retained a normal karyotype; and were capable of forming teratomas. DNA microarray analysis was used to compare the transcriptional profiles of SDEC and the less supportive WI38 and Detroit 551 human cell lines. The mRNA level of secreted frizzledrelated protein (sFRP-1), a known antagonist of the WNT=b-catenin signaling pathway, was significantly reduced in SDEC as compared with the other 2 cell lines, whereas the mRNA levels of prostaglandin-endoperoxide synthase 2 (PTGS2 or COX-2) and prostaglandin I 2 synthase (PGIS), two prostaglandin biosynthesis genes, were significantly increased in SDEC. The level of sFRP-1 protein was significantly reduced, and levels of 2 prostaglandins that are downstream products of PTGS2 and PGIS, prostaglandin E 2 and 6-keto-prostaglandin F 1a , were significantly elevated in SDEC CM compared with WI38, Detroit 551, and MEF CM. Further, addition of purified sFRP-1 to SDEC CM reduced the proliferation of hESC grown on COL I as well as MAT in a dosedependent manner.

show abstract

“…Fetal kas ve deri hücreleri, sünnet derisi fibroblastları, erişkin fallop tüpü epitel hücreleri, plasenta fibroblastları, amniyotik epitel hücreler, göbek kordonu hücreleri ve koryonik plak hücrelerinin de aralarında bulunduğu bu besleyici hücreler insan embriyonik kök hücrelerin uzun süreli, stabil kültürünü desteklemiştir [28][29][30][31][32][33][34][35]. İn-san kökenli besleyici hücreler kullanılarak yalnızca uzun süreli insan embriyonik kök hücre kültürü değil aynı zamanda yeni insan embriyonik kök hücre dizilerinin derivasyonu da gerçekleştirilmiştir [36][37][38].…”

Section: İnsan Embriyonik Kök Hücre Kültürüunclassified

Human embryonic stem cells and microenvironment

İskender

izgi²,

Şanlıoğlu³

et al. 2014

J Clin Exp Invest

View full text Add to dashboard Cite

Human embryonic stem cells (hESCs) possess a great potential in the field of regenerative medicine by their virtue of pluripotent potential with indefinite proliferation capabilities. They can self renew themselves and differentiate into three embryonic germ layers. Although they are conventionally grown on mitotically inactivated mouse feeder cells, there are in vitro culture systems utilizing feeder cells of human origin in order to prevent cross-species contamination. Recently established in vitro culture systems suggested that direct interaction with feeder cells is not necessary but rather attachment to a substrate is required to ensure long-term, efficient hESC culture in vitro. This substrate is usually composed of a mixture of extracellular matrix components representing in vivo natural niche. In hESC biology, the mechanism of interaction of hESCs with extracellular matrix molecules remained insufficiently explored area of research due to their transient nature of interaction with the in vivo niche. However, an in vitro culture system established using extracellular matrix molecules may provide a safer alternative to culture systems with feeder cells while paving the way to Good Manufacturing Practice-GMP production of hESCs for therapeutic purposes. Therefore, it is essential to study the interaction of extracellular matrix molecules with hESCs in order to standardize in vitro culture systems for large-scale production of hESCs in a less laborintensive way. This would not only provide valuable information regarding the mechanisms that control pluripotency but also serve to dissect the molecular signaling pathways of directed differentiation for prospective therapeutic applications in the future. J Clin Exp Invest 2014; 5 (3): 486-495

show abstract

Cells Derived from Human Umbilical Cord Blood Support the Long-Term Growth of Undifferentiated Human Embryonic Stem Cells

Cited by 9 publications

References 23 publications

Hair follicle-derived mesenchymal cells support undifferentiated growth of embryonic stem cells

Hair follicle-derived mesenchymal cells support undifferentiated growth of embryonic stem cells

Proliferation and Pluripotency of Human Embryonic Stem Cells Maintained on Type I Collagen

Human embryonic stem cells and microenvironment

Contact Info

Product

Resources

About