Cellobiose phosphorylase from Caldicellulosiruptor bescii catalyzes reversible phosphorolysis via different kinetic mechanisms

Bai, Shaowei; Yang, Liangzhen; Wang, Honglei; Yang, Chao; Hou, Xuechen; Gao, Jingjie; Zhang, Zuoming

doi:10.1038/s41598-022-08036-z

Cited by 5 publications

(2 citation statements)

References 49 publications

(59 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The rate limiting step of cellulase hydrolysis to glucose is β-glucoside hydrolyzes cellobiose into two glucose monomers [ 46 ]. Recently, it has been found that cellobiose phosphorylase can provide energy advantage for Cellobiose degradation through phosphorylation pathway [ 47 ]. The inhibition of cellulase and β-glucosidase activity led to a significant decrease in cellobiose content.…”

Section: Discussionmentioning

confidence: 99%

Metabolome and transcriptome analyses reveal changes of rapeseed in response to ABA signal during early seedling development

Chen,

Wu,

et al. 2024

BMC Plant Biol

View full text Add to dashboard Cite

Seed germination is an important development process in plant growth. The phytohormone abscisic acid (ABA) plays a critical role during seed germination. However, the mechanism of rapeseed in response to ABA is still elusive. In order to understand changes of rapeseed under exogenous ABA treatment, we explored differentially expressed metabolites (DEMs) and the differentially expressed genes (DEGs) between mock- and ABA-treated seedlings. A widely targeted LC-MS/MS based metabolomics were used to identify and quantify metabolic changes in response to ABA during seed germination, and a total of 186 significantly DEMs were identified. There are many compounds which are involved in ABA stimuli, especially some specific ABA transportation-related metabolites such as starches and lipids were screened out. Meanwhile, a total of 4440 significantly DEGs were identified by transcriptomic analyses. There was a significant enrichment of DEGs related to phenylpropanoid and cell wall organization. It suggests that exogenous ABA mainly affects seed germination by regulating cell wall loosening. Finally, the correlation analysis of the key DEMs and DEGs indicates that many DEGs play a direct or indirect regulatory role in DEMs metabolism. The integrative analysis between DEGs and DEMs suggests that the starch and sucrose pathways were the key pathway in ABA responses. The two metabolites from starch and sucrose pathways, levan and cellobiose, both were found significantly down-regulated in ABA-treated seedlings. These comprehensive metabolic and transcript analyses provide useful information for the subsequent post-transcriptional modification and post germination growth of rapeseed in response to ABA signals and stresses.

show abstract

Section: Discussionmentioning

confidence: 99%

Metabolome and transcriptome analyses reveal changes of rapeseed in response to ABA signal during early seedling development

Chen,

Wu,

et al. 2024

BMC Plant Biol

View full text Add to dashboard Cite

show abstract

“…In the past three years, some articles have also reported newly discovered phosphorylases [28][29][30], promising in synthesizing rare sugars. In addition to the one-pot enzymatic preparation method, some articles report phosphorylase cascade reactions to prepare target products [25,[31][32][33].…”

Section: Synthesis Of Nigerose By the Coupling Reaction Of Maltose Ph...mentioning

confidence: 99%

Efficient synthesis of nigerose by a novel nigerose phosphorylase from Anaerosporobacter mobilis

et al. 2022

Syst Microbiol and Biomanuf

View full text Add to dashboard Cite

Nigerose is a kind of rare disaccharide connected by an α-1,3 glucosidic bond, which is a potential probiotic due to its antidigestive properties and beneficial functions. This study identified and characterized a novel GH65 glycoside phosphorylase derived from Anaerosporobacter mobilis (AmNP). This new protein could specifically catalyze the phospholysis of nigerose to generate glucose and glucose-1-phosphate in the presence of phosphate, indicating it was a typical nigerose phosphorylase. Compared to the previously reported nigerose phosphorylases, AmNP exhibited lower affinity towards nigerose in phosphorolysis reaction and higher affinity towards glucose in reverse phosphorolysis reaction, which indicated that AmNP might be superior in the synthetic capability of disaccharide. Then AmNP was employed to synergize with maltose phosphorylase from Lactobacillus brevis (LbMP) to catalyze the synthesis of nigerose using maltose as the substrate. After optimization of reaction conditions, the highest nigerose yield reached 132.0 g/L with a 66.3% conversion rate, which was higher than ever reported cases using the same reaction pathway to our knowledge. These findings on AmNP in this work were expected to provide a new candidate for large-scale enzymatic synthesis of nigerose and have important theoretical significance for studying nigerose phosphorylase.

show abstract

Recent advances in genetic engineering to enhance plant-polysaccharide-degrading enzyme expression in Penicillium oxalicum: A brief review

Hu,

Liu,

Ouyang

et al. 2024

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

Cellobiose phosphorylase from Caldicellulosiruptor bescii catalyzes reversible phosphorolysis via different kinetic mechanisms

Cited by 5 publications

References 49 publications

Metabolome and transcriptome analyses reveal changes of rapeseed in response to ABA signal during early seedling development

Metabolome and transcriptome analyses reveal changes of rapeseed in response to ABA signal during early seedling development

Efficient synthesis of nigerose by a novel nigerose phosphorylase from Anaerosporobacter mobilis

Recent advances in genetic engineering to enhance plant-polysaccharide-degrading enzyme expression in Penicillium oxalicum: A brief review

Contact Info

Product

Resources

About