Cellobiose dehydrogenase and chitosan‐based lysozyme responsive materials for antimicrobial wound treatment

Öhlknecht, Christoph; Tegl, Gregor; Beer, Bianca; Sygmund, Christoph; Ludwig, Roland; Guebitz, Georg M.

doi:10.1002/bit.26070

Cited by 24 publications

(19 citation statements)

References 23 publications

(29 reference statements)

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“…Existing studies on the inhibitory effects of chitosan against E. coli and S. aureus do not reveal a significant difference in the susceptibility of these two bacteria towards chitosan [29]. The H 2 O 2 concentration generated by GOX in the culture media was measured to be between 55 and 68 μM within 8 h incubation whereby a steady state of this concentration range was obtained after 2 h. This concentrations are in agreement with previous studies of immobilized H 2 O 2 producing enzymes [19,30,31] and lie above the desired values of 10 μM which are known to be the minimum inhibitory concentration [32]. The culture media were incubated with the particles for 24 h and the complete inhibition of bacterial growth could be set upright long this time, proofing a high stability of immobilized GOX in this complex medium.…”

Section: Antioxidant and Antimicrobial Propertiessupporting

confidence: 90%

The chemo enzymatic functionalization of chitosan zeolite particles provides antioxidant and antimicrobial properties

Tegl

Stagl

Mensah

et al. 2018

Engineering in Life Sciences

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Silicate‐based microporous materials like zeolites are nano enabled particles and used for various applications including pharmaceutical formulations. This study reports on the chemo‐enzymatic functionalization of chitosan‐zeolite particles (CTS‐zeolites) with caffeic acid (CA) and glucose oxidase (GOX) to impart combined antioxidant and antimicrobial properties. CA was grafted on the chitosan moieties by using laccase generating stable particles (zeta potential –36.7 mV) of high antioxidant activity (44% DPPH inhibition). GOX was immobilized both on CTS‐zeolites and on CA modified CTS‐zeolites and creating a hydrogen peroxide generation system continuously and in‐situ producing this oxidative and antimicrobial agent. The system prevented bacterial growth of E. coli and S. aureus over 24 h whereby a steady‐state concentration of around 60 μM hydrogen peroxide in the culture medium was observed. CA and GOX functionalized CTS‐zeolite particles additionally showed combinatorial antioxidant and antimicrobial properties providing a powerful bioactive system for medical applications. These particles proved their suitability for incorporation in bioactive formulations which could be used, inter alia, for topical wound treatments.

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Section: Antioxidant and Antimicrobial Propertiessupporting

confidence: 90%

The chemo enzymatic functionalization of chitosan zeolite particles provides antioxidant and antimicrobial properties

Tegl

Stagl

Mensah

et al. 2018

Engineering in Life Sciences

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“…Worldwide, chitosan can be accumulated for 6–8 million tons every year and contain various bio-functional groups such as –NH 2 and –OH 2 . It has been widely applied in various fields, such as artificial skin 3 , food and nutrition 4 , ophthalmology 5 , textile finishing 6 , batteries 7 , 8 , drug-delivery system 9 , biotechnology 10 , 11 , etc.…”

Section: Introductionmentioning

confidence: 99%

Hierarchical Porous Chitosan Sponges as Robust and Recyclable Adsorbents for Anionic Dye Adsorption

Wang

Sun

et al. 2017

Sci Rep

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Biomass waste treatment and detrimental dye adsorption are two of the crucial environmental issues nowadays. In this study, we investigate to simultaneously resolve the aforementioned issues by synthesizing chitosan sponges as adsorbents toward rose bengal (RB) dye adsorption. Through a temperature-controlled freeze-casting process, robust and recyclable chitosan sponges are fabricated with hierarchical porosities resulted from the control of concentrations of chitosan solutions. Tested as the adsorbents for RB, to the best of our knowledge, the as-prepared chitosan sponge in this work reports the highest adsorption capacity of RB (601.5 mg/g) ever. The adsorption mechanism, isotherm, kinetics, and thermodynamics are comprehensively studied by employing statistical analysis. Importantly and desirably, the sponge type of chitosan adsorbents exceedingly facilitates the retrieving and elution of chitosan sponges for recyclable uses. Therefore, the chitosan sponge adsorbent is demonstrated to possess dramatically squeezable capability with durability for 10,000 cycles and recyclable adsorption for at least 10 cycles, which provides an efficient and economical way for both biomass treatment and water purification.

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“…This was a sufficient concentration to induce an antimicrobial effect against S . aureus and E. coli (Figure 10-c) [305].…”

Section: Nanotechnology For Treatment Of Burn Infections and Woundmentioning

confidence: 99%

Nanomedicine and advanced technologies for burns: Preventing infection and facilitating wound healing

Jahromi

Zangabad

Basri

et al. 2018

Advanced Drug Delivery Reviews

349

189

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According to the latest report from the World Health Organization, an estimated 265,000 deaths still occur every year as a direct result of burn injuries. A widespread range of these deaths induced by burn wound happens in low- and middle-income countries, where survivors face a lifetime of morbidity. Most of the deaths occur due to infections when a high percentage of the external regions of the body area is affected. Microbial nutrient availability, skin barrier disruption, and vascular supply destruction in burn injuries as well as systemic immunosuppression are important parameters that cause burns to be susceptible to infections. Topical antimicrobials and dressings are generally employed to inhibit burn infections followed by a burn wound therapy, because systemic antibiotics have problems in reaching the infected site, coupled with increasing microbial drug resistance. Nanotechnology has provided a range of molecular designed nanostructures (NS) that can be used in both therapeutic and diagnostic applications in burns. These NSs can be divided into organic and non-organic (such as polymeric nanoparticles (NPs) and silver NPs, respectively), and many have been designed to display multifunctional activity. The present review covers the physiology of skin, burn classification, burn wound pathogenesis, animal models of burn wound infection, and various topical therapeutic approaches designed to combat infection and stimulate healing. These include biological based approaches (e.g. immune-based antimicrobial molecules, therapeutic microorganisms, antimicrobial agents, etc.), antimicrobial photo- and ultrasound-therapy, as well as nanotechnology-based wound healing approaches as a revolutionizing area. Thus, we focus on organic and non-organic NSs designed to deliver growth factors to burned skin, and scaffolds, dressings, etc. for exogenous stem cells to aid skin regeneration. Eventually, recent breakthroughs and technologies with substantial potentials in tissue regeneration and skin wound therapy (that are as the basis of burn wound therapies) are briefly taken into consideration including 3D-printing, cell-imprinted substrates, nano-architectured surfaces, and novel gene-editing tools such as CRISPR-Cas.

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Cellobiose dehydrogenase and chitosan‐based lysozyme responsive materials for antimicrobial wound treatment

Cited by 24 publications

References 23 publications

The chemo enzymatic functionalization of chitosan zeolite particles provides antioxidant and antimicrobial properties

The chemo enzymatic functionalization of chitosan zeolite particles provides antioxidant and antimicrobial properties

Hierarchical Porous Chitosan Sponges as Robust and Recyclable Adsorbents for Anionic Dye Adsorption

Nanomedicine and advanced technologies for burns: Preventing infection and facilitating wound healing

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