CellCountCV – a web-application for accurate cell counting and automated batch processing of microscopy images using fully-convolutional neural networks

Abstract

The in vitro cellular models are promising tools for studying normal and pathological conditions. One of their important applications is the development of genetically engineered biosensor systems to investigate the processes occurring in living cells in real time. Today, there are fluorescence protein based sensory systems for detecting various substances in living cells (for example, hydrogen peroxide, ATP, Ca 2+ etc.) or for detecting processes such as endoplasmic reticulum stress. Such systems help to study mechanisms underlying the pathogenic processes and diseases and for screening potential therapeutic compounds. It is also necessary to develop new tools for processing and analysis of obtained microimages. Here we present our web-application CellCountCV for automation of microscopy cell images analysis which is based on fully-convolutional deep neural networks. This approach can efficiently deal with non-convex overlapping objects, that are virtually inseparable with conventional image processing methods. The cell counts predicted with CellCountCV were very close to expert estimates (the average error rate was < 4%). CellCountCV was used to analyse large series of microscopy images obtained in experimental studies and it was able to demonstrate the endoplasmic reticulum stress development and to catch the dose-dependent effect of tunicamycin.

Algorithm for automatic detection of insulin granule exocytosis in human pancreatic β-cells

“…Existing platforms like Fiji, Metamorph and many more, while useful, lack the versatility required for diverse biological analyses. Recent developments have seen the emergence of tools specifically designed for exocytosis event analysis (8,11,12).…”

“…Existing platforms like Fiji, Metamorph and many more, while useful, lack the versatility required for diverse biological analyses. Recent developments have seen the emergence of tools specifically designed for exocytosis event analysis (8,11,12). Our work diverges from these complex methodologies, aiming instead for a simplified, scalable, and rapid detection of exocytosis.…”