Cell Wall β-(1,6)-Glucan of Saccharomyces cerevisiae

Aimanianda, Vishukumar; Clavaud, Cécile; Simenel, Catherine; Fontaine, Thierry; Delepierre, Muriel; Latgé, Jean‐Paul

doi:10.1074/jbc.m807667200

Cited by 119 publications

(64 citation statements)

References 57 publications

(41 reference statements)

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“…RodAp was extracted from the spore surface by incubating 10 9 dry conidia with 48% (vol/vol) hydrofluoric acid (HF) for 72 h at 4°C (23). The contents were centrifuged (10,000 ϫ g, 10 min), and the supernatant obtained was dried under N 2 .…”

Section: Methodsmentioning

confidence: 99%

Surface Structure Characterization of Aspergillus fumigatus Conidia Mutated in the Melanin Synthesis Pathway and Their Human Cellular Immune Response

et al. 2014

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gIn Aspergillus fumigatus, the conidial surface contains dihydroxynaphthalene (DHN)-melanin. Six-clustered gene products have been identified that mediate sequential catalysis of DHN-melanin biosynthesis. Melanin thus produced is known to be a virulence factor, protecting the fungus from the host defense mechanisms. In the present study, individual deletion of the genes involved in the initial three steps of melanin biosynthesis resulted in an altered conidial surface with masked surface rodlet layer, leaky cell wall allowing the deposition of proteins on the cell surface and exposing the otherwise-masked cell wall polysaccharides at the surface. Melanin as such was immunologically inert; however, deletion mutant conidia with modified surfaces could activate human dendritic cells and the subsequent cytokine production in contrast to the wild-type conidia. Cell surface defects were rectified in the conidia mutated in downstream melanin biosynthetic pathway, and maximum immune inertness was observed upon synthesis of vermelone onward. These observations suggest that although melanin as such is an immunologically inert material, it confers virulence by facilitating proper formation of the A. fumigatus conidial surface.

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Section: Methodsmentioning

confidence: 99%

Surface Structure Characterization of Aspergillus fumigatus Conidia Mutated in the Melanin Synthesis Pathway and Their Human Cellular Immune Response

et al. 2014

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“…MALDI-TOF mass spectra were acquired on a Voyager Elite DE-STR mass spectrometer (Perspective Biosystems, Framingham, MA) equipped with a pulsed nitrogen laser (337 nm) and a gridless delayed extraction ion source. The spectrometer was operated in positive reflectron mode as previously described (22).…”

Section: Methodsmentioning

confidence: 99%

Characterization of a New β(1–3)-Glucan Branching Activity of Aspergillus fumigatus

Gastebois

Mouyna

Simenel

et al. 2010

Journal of Biological Chemistry

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“…They constitute approximately 50–60% of the dry weight of fungal cell walls [10] and thereof approximately 65–90% are β-(1,3)-glucans and approximately 10–15% are β-(1,6)-glucans [11,12]. The β-(1,3)-glucans are well-known pro-inflammatory agents [13,14] and innate immune responses can be induced through binding to monocytes or macrophages via the receptor dectin-1 [15] or complement receptor 3 (CR3) [16].…”

Section: Introductionmentioning

confidence: 99%

IgG to Various Beta-Glucans in a Human Adult Population

Noss¹,

Wouters²,

Smit³

et al. 2011

Int Arch Allergy Immunol

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Background: Fungal β-(1,3)-glucans are pro-inflammatory agents, and exposures to β-(1,3)-glucans are associated with respiratory tract symptoms. IgG anti-(1,3)-glucan titers are measured in diagnosis of fungal infections. Although other β-glucan structures exist, like β-(1,6)-glucans, little is known about their antigenic or pro-inflammatory properties. We aimed to investigate IgG titers and specificities in human sera against different β-glucans with varying structures. Methods: IgG anti-β-glucan was measured by enzyme immunoassay in a random sample of 40 sera from healthy adults, with a panel of 8 differently structured glucans. In a subsequent larger series, IgG anti-β-(1,6)-glucan was measured in a random sample of 667 sera from three occupational populations with different organic dust exposures. Possible determinants of IgG anti-β-(1,6)-glucan titers were explored with linear-regression analysis. Results: We found wide variation in anti-β-glucan IgG levels. The highest titers were found for pure β-(1,6)-glucan pustulan. Moderate to strong reactions with other β-(1,6)-containing structures appeared to be due to cross-reacting anti-β-(1,6)-glucan antibodies. Surprisingly, the mean IgG anti-β-(1,6)-glucan titer was significantly lower in agricultural workers – with highest organic dust exposure – than in spray painters and bakery workers. Smoking status was associated with lower IgG anti-β-(1,6)-glucan titers in all populations. Conclusions: IgG to β-(1,3)- and β-(1,6)-glucans can be found in normal human sera. β-(1,6)-glucans appear to be much more potent antigens. The health impact of high anti-β-(1,6)-glucan antibody levels remains unclear and further investigations are needed.

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Cell Wall β-(1,6)-Glucan of Saccharomyces cerevisiae

Cited by 119 publications

References 57 publications

Surface Structure Characterization of Aspergillus fumigatus Conidia Mutated in the Melanin Synthesis Pathway and Their Human Cellular Immune Response

Surface Structure Characterization of Aspergillus fumigatus Conidia Mutated in the Melanin Synthesis Pathway and Their Human Cellular Immune Response

Characterization of a New β(1–3)-Glucan Branching Activity of Aspergillus fumigatus

IgG to Various Beta-Glucans in a Human Adult Population

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