Cell wall surface layer (S-layer) promotes colony formation in Microcystis: comparison of S-layer characteristics between colonial and unicellular forms of Microcystis and function conformation

Zu, Yao; Hong, Sujuan; Xu, Chongxin; Li, Weiwei; Chen, Siyu; Li, Jianhong

doi:10.1007/s11356-020-08254-w

Cited by 10 publications

(2 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The TB-EPS on cells was not completely removed ( Figure 5B ). Most Microcystis (>85%) have a layer of hydrophobic proteins outside the cell wall named S-layer, which is important for cell adhesion and surface recognition ( Šmarda et al., 2002 ; Schachtsiek et al., 2004 ; Zu et al., 2020 ). This structure may overlap with TB-EPS, which allows cells in group CK to directly recognize and adhere to special substances between TB-EPS, thus forming larger and relatively denser colonies.…”

Section: Discussionmentioning

confidence: 99%

Effect of extracellular polymeric substances on the colony size and morphological changes of Microcystis

Pan,

Yang,

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

Surface blooms of colony-forming Microcystis are increasingly occurring in aquatic ecosystems on a global scale. Recent studies have found that the Microcystis colonial morphology is a crucial factor in the occurrence, persistence, and dominance of Microcystis blooms, yet the mechanism driving its morphological dynamics has remained unknown. This study conducted a laboratory experiment to test the effect of extracellular polymeric substances on the morphological dynamics of Microcystis. Ultrasound was used to disaggregate colonies, isolating the cells and of the Microcystis suspension. The single cells were then re-cultured under three homologous EPS concentrations: group CK, group Low, and group High. The size, morphology, and EPS [including tightly bound EPS (TB-EPS), loosely bound EPS (LB-EPS), bound polysaccharides (B-polysaccharides), and bound proteins (B-proteins)] changes of colonies were closely monitored over a period of 2 months. It was observed that colonies were rapidly formed in group CK, with median colony size (D50) reaching 183 µm on day 12. The proportion of colonies with a size of 150–500 µm increased from 1% to more than 50%. Colony formation was also observed in both groups Low and High, but their D50 increased at a slower rate and remained around 130 µm after day 17. Colonies with a size of 50–150 µm account for more than 50%. Groups CK and Low successively recovered the initial Microcystis morphology, which is a ring structure formed of several small colonies with a D50 of 130 µm. During the recovery of the colony morphology, the EPS per cell increased and then decreased, with TB-EPS and B-polysaccharides constituting the primary components. The results suggest that colony formation transitioned from adhesion driven to being division driven over time. It is suggested that the homologous EPS released into the ambient environment due to the disaggregation of the colony is a chemical cue that can affect the formation of a colony. This plays an important but largely ignored role in the dynamics of Microcystis and surface blooms.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of extracellular polymeric substances on the colony size and morphological changes of Microcystis

Pan,

Yang,

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…In laboratory enrichment cultures Methylomirabilis bacteria prefer to grow in aggregates with other microorganisms rather than planktonically ( Gambelli et al, 2018 ). S-layers have been implicated to promote formation and maintenance of the aggregates ( Zu et al, 2020 ), the flat surfaces of the cell walls of Methylomirabilis bacteria might aid in the attachment of one cell to the other. Moreover, flat surfaces and aggregates could help against shearing forces that these microorganisms might be exposed to in their natural habitats ( Mader et al, 1999 ).…”

Section: Discussionmentioning

confidence: 99%

The Polygonal Cell Shape and Surface Protein Layer of Anaerobic Methane-Oxidizing Methylomirabilislanthanidiphila Bacteria

et al. 2021

View full text Add to dashboard Cite

Methylomirabilis bacteria perform anaerobic methane oxidation coupled to nitrite reduction via an intra-aerobic pathway, producing carbon dioxide and dinitrogen gas. These diderm bacteria possess an unusual polygonal cell shape with sharp ridges that run along the cell body. Previously, a putative surface protein layer (S-layer) was observed as the outermost cell layer of these bacteria. We hypothesized that this S-layer is the determining factor for their polygonal cell shape. Therefore, we enriched the S-layer from M. lanthanidiphila cells and through LC-MS/MS identified a 31 kDa candidate S-layer protein, mela_00855, which had no homology to any other known protein. Antibodies were generated against a synthesized peptide derived from the mela_00855 protein sequence and used in immunogold localization to verify its identity and location. Both on thin sections of M. lanthanidiphila cells and in negative-stained enriched S-layer patches, the immunogold localization identified mela_00855 as the S-layer protein. Using electron cryo-tomography and sub-tomogram averaging of S-layer patches, we observed that the S-layer has a hexagonal symmetry. Cryo-tomography of whole cells showed that the S-layer and the outer membrane, but not the peptidoglycan layer and the cytoplasmic membrane, exhibited the polygonal shape. Moreover, the S-layer consisted of multiple rigid sheets that partially overlapped, most likely giving rise to the unique polygonal cell shape. These characteristics make the S-layer of M. lanthanidiphila a distinctive and intriguing case to study.

show abstract

Editorial special issue on cyanobacterial blooms and water ecological restoration

2020

Environ Sci Pollut Res

View full text Add to dashboard Cite

Cyanobacterial bloom formation and aquatic ecosystem degradation driven by global warming and eutrophication have become serious ecological and environmental problems worldwide. Recently, great progress in physiology of cyanobacteria has been achieved, but our knowledge on the mechanisms of bloom formation of cyanobacterial species and the feasible methods for ecological restoration is still poor. The 6th Forum on Cyanobacterial Blooms, organized by the Northwest Agriculture and Forest University, Yangling, China, in 22-23 June 2019, was held to share most recent research progress in cyanobacterial blooms and ecological restoration around China. This forum was held annually since 2014. The forum was jointly proposed and initiated by Prof. Guoxiang Wang from Nanjing Normal University and Prof. Renhui Li from the Institute of Hydrobiology, Chinese Academy of Sciences. Since then, the form has covered a wide variety of topics, including the most common toxic bloomforming cyanobacterium Microcystis, cyanobacteria blooms and nutrient cycling in shallow lakes, interactions between cyanobacterial bloom formation and physical, chemical, and biological factors, as well as monitoring and early warning of cyanobacterial blooms. This Special Issue of Environmental Science and Pollution Research comprises selected papers presented in the 6th Forum on Cyanobacterial Blooms. In detail, 13 communications are presented in this special issue, elucidating the following topics:

show abstract

Cell wall surface layer (S-layer) promotes colony formation in Microcystis: comparison of S-layer characteristics between colonial and unicellular forms of Microcystis and function conformation

Cited by 10 publications

References 50 publications

Effect of extracellular polymeric substances on the colony size and morphological changes of Microcystis

Effect of extracellular polymeric substances on the colony size and morphological changes of Microcystis

The Polygonal Cell Shape and Surface Protein Layer of Anaerobic Methane-Oxidizing Methylomirabilislanthanidiphila Bacteria

Editorial special issue on cyanobacterial blooms and water ecological restoration

Contact Info

Product

Resources

About