Cell wall structure of secreted laccase-silenced strain in Lentinula edodes

Sakamoto, Yuichi; Nakade, Keiko; Sato, Shiho; Yoshimi, Akira; Sasaki, Kuniaki; Konno, Naotake; Abe, Keietsu

doi:10.1016/j.funbio.2018.09.005

Cited by 26 publications

(34 citation statements)

References 51 publications

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“…The mycelial density of the L. edodes isolates also did not present a significant difference between the treatments. Mycelial growth was characterized by the presence of low density mycelium (note 1), even after 23 days of cultivation (Table 1), which differs from the results cited by Sakamoto et al (2018) with L. edodes with increased of the cultivation period.…”

Section: Mycelial Growth Of Lentinula Edodescontrasting

confidence: 74%

“…The formation of the compact composites by L. edodes can be correlated with the aggregation of the culture particles substrate by the chitin (Islam et al, 2017;Yang et al, 2017) or by the increase in β-1,3-1,6-glucan present on the fungal cell wall (Sakamoto et al, 2018), which can be considered as a natural polymer and responsible for the resistance of the fungal composite (Haneef et al, 2017;Yang et al, 2017;Tudryn et al, 2018) Moreover, L. edodes fungi releases oxidative enzymes, such as laccases, to promote the decomposition of the substrate culture favoring mycelial growth (Sakamoto et al, 2018), which reduces the biomass of the vegetal residue (Bento;Casaril, 2012;Ramirez-Chan et al, 2014). Sales-Campos and Andrade (2011) mentioned that the Lentinus strigosus fungi resulted in loss of mass of the substrate culture between 42.2% and 58.6% of wood residues.…”

Section: Mechanical Strength Of Shiitake Mushroom Compositementioning

confidence: 99%

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Production of biocomposites from the reuse of coconut powder colonized by Shiitake mushroom

et al. 2019

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The demand for biodegradable composite has grown worldwide in recent years, mainly in order to reduce environmental contamination by structural materials produced from the oil industry. The objective of this study was to evaluate the growth of isolates from the edible mushroom “Shiitake” (Lentinula edodes) in substrate coconut powder-based supplemented with wheat bran, as well as to analyze the influence of fungi growing period and drying time of the colonized substrate on the mechanical properties of the composite, in order to produce a biodegradable composite. The mycelial density is not influenced by the type of hyphae of L. edodes. Drying of the composite does not influence the residual odor, depending on the isolate. The compressive strength and foam type of the fungal composite may be influenced by the culture period and type of hyphae, depending on the fungal isolate. The composites colonized by the L. edodes isolates presented higher mechanical resistance at 30 days of complete colonization. The coconut powder supplemented with wheat bran colonized by isolated fungi LED 96/18 is an ecological alternative in the packaging production considering its mechanical properties.

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Section: Mycelial Growth Of Lentinula Edodescontrasting

confidence: 74%

Section: Mechanical Strength Of Shiitake Mushroom Compositementioning

confidence: 99%

Production of biocomposites from the reuse of coconut powder colonized by Shiitake mushroom

et al. 2019

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“…Fungal cell wall variation is a vital factor influencing pellet morphology and changes in laccase secretion. 29,31 The thicknesses of the hyphal walls of all three recombinant strains were observed using TEM (Figure 7). The thickness of the P. ferulae::sspoxa3a/b cell wall (0.090 ± 0.017 μm) was approximately 0.80-fold that of the control cell wall (0.112 ± 0.009 μm).…”

Section: ■ Resultsmentioning

confidence: 99%

Roles of Small Subunits of Laccase (ssPOXA3a/b) in Laccase Production by Pleurotus eryngii var. ferulae

Zhang

Yuan

Wang

et al. 2021

J. Agric. Food Chem.

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The small subunit, ssPOXA3a/b, and the large subunit, POXA3, are indispensable components of typical heterodimeric laccase (Lacc2) in white rot fungi. However, the enzymatic and biological functions of ssPOXA3a/b remain unclear. The present study revealed that neither ssPOXA3a nor ssPOXA3b per se has a catalytic ability, whereas their combination with POXA3 (and especially ssPOXA3b) enhances the activity, thermostability, and pH stability of POXA3. In Pleurotus eryngii var. ferulae, there was no regulatory relationship between ssPOXA3a/b and POXA3 at the transcriptional level. However, sspoxa3a/b overexpression had a negative feedback effect on lacc6 transcription. By contrast, poxa3 transcripts had no effect on any other laccase isoenzyme. Overexpression of sspoxa3a/b resulted in small fungal pellets, thin mycelial walls, and facilitated laccase secretion. However, poxa3 overexpression had no influence on pellet morphology. Collectively, this work elucidated the functions of ssPOXA3a/b and laid an empirical foundation for the development of high-yield laccase.

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“…6,25 Moreover, appressorial turgor pressure provides power for hyphal penetration through the host insect cuticle. 49 In ΔMacwh mutants, appressorial formation rate and the turgor pressure were significantly reduced, accompanied by sets of down-regulated genes involved in attachment, such as those encoding hydrophobins (EFY89521, EFY86282) 6 and filamentous hemagglutinin/adhesin (EFY85044), 50 and those involved in cuticular penetration, such as those encoding tetraspanin (EFY93077), 27 laccases (EFY89448, EFY88619), [51][52][53] endochitinase CHI3 (EFY86188) 54 and subtilisin (EFY85466). 55 In conclusion, Macwh genes play important roles in cell wall construction and integrity, and contribute to virulence by affecting spore attachment, appressorial formation and turgor pressure.…”

Section: Discussionmentioning

confidence: 99%

Calcofluor white hypersensitive proteins contribute to stress tolerance and pathogenicity in entomopathogenic fungus, Metarhizium acridum

Yan

Chen

et al. 2020

Pest Management Science

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BACKGROUND Fungal cell wall integrity is vital for fungal pathogenesis and stress tolerance. Calcofluor white (CFW), a cell wall perturbing agent, inhibits fungal growth by binding chitin in the cell wall. The roles of CFW sensitive proteins remain insufficiently understood in pathogenic fungi. RESULTS We investigated two calcofluor white hypersensitive proteins, MaCwh1 and MaCwh43, in the entomopathogenic fungus Metarhizium acridum. Both Green fluorescent protein (GFP)‐tagged MaCwh1 and MaCwh43 localized at the endoplasmic reticulum. Our results showed that the ΔMacwh1 and ΔMacwh43 mutants were more sensitive to CFW and ultraviolet irradiation stress compared to wild‐type and complement strains. ΔMacwh1 had a stronger sensitivity to these stresses than ΔMacwh43. Both ΔMacwh1 and ΔMacwh43 mutants showed smoother cell wall surface, and drastically reduced chitin and mannose glycoprotein level in the cell wall and glycerol level in conidia compared to wild type. Insect bioassay showed significantly attenuated virulence for both ΔMacwh1 and ΔMacwh43 mutants with impaired ability in penetrating the host cuticle. RNA‐Seq analysis revealed that a large number of genes presumably involved in cell wall construction and modification, pathogenicity and stress response were down‐regulated in both ΔMacwh1 and ΔMacwh43 mutants. CONCLUSIONS These findings demonstrate that both Macwh1 and Macwh43 affect the fungal cell wall ultrastructure and contribute to the stress tolerance and pest control potential in M. acrdium. © 2020 Society of Chemical Industry

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Cell wall structure of secreted laccase-silenced strain in Lentinula edodes

Cited by 26 publications

References 51 publications

Production of biocomposites from the reuse of coconut powder colonized by Shiitake mushroom

Production of biocomposites from the reuse of coconut powder colonized by Shiitake mushroom

Roles of Small Subunits of Laccase (ssPOXA3a/b) in Laccase Production by Pleurotus eryngii var. ferulae

Calcofluor white hypersensitive proteins contribute to stress tolerance and pathogenicity in entomopathogenic fungus, Metarhizium acridum

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