Cell Wall Reactive Proteins in the Coat and Wall of Maize Pollen

Suen, Der Fen; Wu, Sherry; Chang, Han Chang; Dhugga, Kanwarpal S.; Huang, Anthony H. C.

doi:10.1074/jbc.m307843200

Cited by 64 publications

(41 citation statements)

References 36 publications

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“…In comparison, maize pollen, although having a shape similar to European native grass pollen, is about three times larger in diameter and thus possesses some 27-fold higher volume and probably weight than grass pollen. Second, pollen grains of maize are more sticky due to pollenkitt present on the exine surface, which causes agglomeration of the pollen and may prevent aerial spread even further [40]. However, pollen fragments, such as starch granules, may be released and distributed as published by Suphioglu et al [41], which may be of importance considering the tremendous amounts of maize pollen produced in agriculture.…”

Section: Discussionmentioning

confidence: 99%

Proteome analysis of maize pollen for allergy-relevant components

et al. 2006

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Over the last few decades, the cultivation of maize (Zea mays) has strongly increased in Central Europe. We therefore decided to study the allergen composition and the allergenic potency of its pollen in comparison with pollen from timothy grass (Phleum pratense), a typical representative of the native grasses. We found that 65% of the sera reactive to timothy pollen also bound to maize pollen proteins. By using 2-DE immunoblotting, followed by incubation with mAbs directed against known allergens or protein sequencing, those IgE-reactive components were further classified. Although novel, maize-specific pollen allergens could not be found, the presence of crossreacting allergens belonging to groups 1 and 13 (Zea m 1 and 13), both having high IgE prevalence, as well as the presence of the less important group 3 and 12 allergens was found. The structural variability of Zea m 1 and Zea m 13 was determined by sequencing clones isolated from a maize pollen cDNA library. This revealed sequence identities of 72 and 70%, respectively, to the corresponding Phl p 1 and Phl p 13 allergens of timothy grass pollen. IgE-crossreactivity was further studied using immunoblot inhibition tests. Here, timothy pollen extract completely blocked IgE binding to maize, whereas maize pollen extract blocked IgE reactivity to only some timothy pollen allergens.

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Section: Discussionmentioning

confidence: 99%

Proteome analysis of maize pollen for allergy-relevant components

et al. 2006

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“…During pollen development, a thick callose wall composed of b-1, 3-glucan has been shown to surround the tetrads (Bucciaglia and Smith, 1994). The b-1, 3-glucanase may play a role in the release of tetrads to free microspores after hydrolysis of the callose (Bucciaglia and Smith, 1994;Tsuchiya et al, 1995;Bucciaglia et al, 2003;Suen et al, 2003). Antisense approach of b-1, 3-glucanase (Tag1) with anther specific RNA reduced the protein level, but showed normal tetrad dissolution and pollen development (Bucciaglia et al, 2003).…”

Section: Genes Contributing To the Male Gametophytic Defect In Osnop mentioning

confidence: 97%

The Oryza sativa no pollen (Osnop) gene plays a role in male gametophyte development and most likely encodes a C2-GRAM domain-containing protein

2005

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Phenotype screens of Ds insertional lines identified a male sterile Orysa sativa no pollen (Osnop) mutant with a pollen-less phenotype at the flowering stage. The mutant phenotype showed linkage to Ds insertion into Osnop gene region. This mutant contained a deletion of 65 kb chromosomal region at the site of Ds insertion containing 14 predicted genes. Out of these deleted genes, Delegen 5-7, 9-10 were redundant, as two or three copies were present with 100% homology in other regions of rice genome. RT-PCR analysis showed that Delegen 5-7 were expressed not only in wild type plants but also in the mutant plants. In addition to this, Delegen 8-10 transcripts could not be detected under normal growth conditions, and Delegen 12 was expressed only in roots, thus deletion of these genes may not affect the pollen development. Our data and analysis also ruled out the possibility of delegen 1-4, 11, and 13 as candidates contributing to the pollen-less phenotype. Further investigation showed that the delegen 14 was expressed only in late stage of pollen development with the highest expression at the stage of pollen release and germination by RT-PCR, Northern blotting, in situ hybridization, and promoter-GUS transgenic plants. Thus, the delegen 14 gene is the best candidate for Osnop, corresponding to the pollen-less phenotype in the mutant. Our data suggest that delegen 14 may play an important role during late stage of pollen development and its germination. Since the delegen 14 gene has both C(2) and GRAM domains, it can be assumed that this gene cross-links both calcium and phosphoinositide signaling pathways. This is the first report to suggest possible functions for this gene in plant development.

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“…On average, the sequence identity was found to be about 60%; the region of homology covers the whole sequence of group 2/3 allergens (expansinlike proteins) and the C-terminal (CBM) sequence of group I allergens (␤-expansin) (48). A 10-kDa expansin-like allergen derived from maize pollen has already been implicated in the past as part of the group of proteins involved in the hydrolysis of style cell walls during pollen tube penetration (190). Although the specific carbohydrate binding determinant of expansins remains controversial, its ability to bind to cellulose was demonstrated.…”

Section: 2003)mentioning

confidence: 99%

Carbohydrate Binding Modules: Biochemical Properties and Novel Applications

Shoseyov

Shani

Levy

2006

Microbiol Mol Biol Rev

479

347

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SUMMARY Polysaccharide-degrading microorganisms express a repertoire of hydrolytic enzymes that act in synergy on plant cell wall and other natural polysaccharides to elicit the degradation of often-recalcitrant substrates. These enzymes, particularly those that hydrolyze cellulose and hemicellulose, have a complex molecular architecture comprising discrete modules which are normally joined by relatively unstructured linker sequences. This structure is typically comprised of a catalytic module and one or more carbohydrate binding modules (CBMs) that bind to the polysaccharide. CBMs, by bringing the biocatalyst into intimate and prolonged association with its substrate, allow and promote catalysis. Based on their properties, CBMs are grouped into 43 families that display substantial variation in substrate specificity, along with other properties that make them a gold mine for biotechnologists who seek natural molecular “Velcro” for diverse and unusual applications. In this article, we review recent progress in the field of CBMs and provide an up-to-date summary of the latest developments in CBM applications.

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Cell Wall Reactive Proteins in the Coat and Wall of Maize Pollen

Cited by 64 publications

References 36 publications

Proteome analysis of maize pollen for allergy-relevant components

Proteome analysis of maize pollen for allergy-relevant components

The Oryza sativa no pollen (Osnop) gene plays a role in male gametophyte development and most likely encodes a C2-GRAM domain-containing protein

Carbohydrate Binding Modules: Biochemical Properties and Novel Applications

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