Cell wall metabolism of peaches and nectarines treated with <scp>UV</scp>‐B radiation: a biochemical and molecular approach

Scattino, Claudia; Negrini, N.; Morgutti, S.; Cocucci, M.; Crisosto, Carlos H.; Tonutti, Pietro; Castagna, Antonella; Ranieri, Annamaria

doi:10.1002/jsfa.7168

Cited by 12 publications

(5 citation statements)

References 74 publications

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“…Therefore, PMEs, together with other cell-wall-dismantling enzymes such as polygalacturonases and β-galactosidases, play a crucial role in the softening process during fruit ripening and senescence, contributing to the overall commercial and organoleptic quality of the products. A UV-B-induced decrease in PME activity in the peel of peach fruit was previously observed [85,86]. Indeed, the authors found a reduction in PME activity 12 and 36 h after a 60 min UV-B exposure, while a decreased PME1 expression was detected 12 and 24 h after the UV-B treatment.…”

Section: Discussionsupporting

confidence: 63%

Transcriptomic Analysis on the Peel of UV-B-Exposed Peach Fruit Reveals an Upregulation of Phenolic- and UVR8-Related Pathways

Santin

Simoni

Vangelisti

et al. 2023

Plants

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UV-B treatment deeply influences plant physiology and biochemistry, especially by activating the expression of responsive genes involved in UV-B acclimation through a UV-B-specific perception mechanism. Although the UV-B-related molecular responses have been widely studied in Arabidopsis, relatively few research reports deepen the knowledge on the influence of post-harvest UV-B treatment on fruit. In this work, a transcriptomic approach is adopted to investigate the transcriptional modifications occurring in the peel of UV-B-treated peach (Prunus persica L., cv Fairtime) fruit after harvest. Our analysis reveals a higher gene regulation after 1 h from the irradiation (88% of the differentially expressed genes—DEGs), compared to 3 h recovery. The overexpression of genes encoding phenylalanine ammonia-lyase (PAL), chalcone syntase (CHS), chalcone isomerase (CHI), and flavonol synthase (FLS) revealed a strong activation of the phenylpropanoid pathway, resulting in the later increase in the concentration of specific flavonoid classes, e.g., anthocyanins, flavones, dihydroflavonols, and flavanones, 36 h after the treatment. Upregulation of UVR8-related genes (HY5, COP1, and RUP) suggests that UV-B-triggered activation of the UVR8 pathway occurs also in post-harvest peach fruit. In addition, a regulation of genes involved in the cell-wall dismantling process (PME) is observed. In conclusion, post-harvest UV-B exposure deeply affects the transcriptome of the peach peel, promoting the activation of genes implicated in the biosynthesis of phenolics, likely via UVR8. Thus, our results might pave the way to a possible use of post-harvest UV-B treatments to enhance the content of health-promoting compounds in peach fruits and extending the knowledge of the UVR8 gene network.

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Section: Discussionsupporting

confidence: 63%

Transcriptomic Analysis on the Peel of UV-B-Exposed Peach Fruit Reveals an Upregulation of Phenolic- and UVR8-Related Pathways

Santin

Simoni

Vangelisti

et al. 2023

Plants

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“…In different peach cultivars and with a 36 h UV-B irradiation, Scattino et al ( 2014 ) showed a different trend of accumulation between hydroxycinnamic acids and flavonols. A positive role of UV-B radiation has been also observed not only as stimulator of antioxidant compounds, but also in extending the shelf-life of post-harvest fruit by lowering the softening process (Scattino et al, 2015 ). However, effects of UV-B radiation can be very different depending on the UV-B dose given, since a long and intense UV-B exposure might induce non-specific stress responses to the plant, while a mild and short UV-B radiation triggers specific adaptation responses, such as the activation of genes specifically involved in UV-B acclimation (Favory et al, 2009 ; Jenkins, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

UV-B Pre-treatment Alters Phenolics Response to Monilinia fructicola Infection in a Structure-Dependent Way in Peach Skin

et al. 2018

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Phenolic compounds represent a large class of secondary metabolites, involved in multiple functions not only in plant life cycle, but also in fruit during post-harvest. phenolics play a key role in the response to biotic and abiotic stresses, thus their accumulation is regulated by the presence of environmental stimuli. The present work aimed to investigate how different pre-UV-B-exposures can modulate the phenolic response of peach fruit infected with Monilinia fructicola. Through HPLC-DAD-MSn, several procyanidins, phenolic acids, flavonols, and anthocyanins were detected. Both UV-B radiation and fungal infection were able to stimulate the accumulation of phenolics, dependent on the chemical structure. Regarding UV-B exposure, inoculated with sterile water, 3 h of UV-B radiation highest concentration of phenolics was found, especially flavonols and cyanidin-3-glucoside far from the wound. However, wounding decreased the phenolics in the region nearby. When peaches were pre-treated with 1 h of UV-B radiation, the fungus had an additive effect in phenolic accumulation far from the infection, while it had a subtractive effect with 3 h of UV-B radiation, especially for flavonols. Canonical discriminant analysis and Pearson correlation revealed that all phenolic compounds, except procyanidin dimer, were highly regulated by UV-B radiation, with particularly strong correlation for quercetin and kaempferol glycosides, while phenolics correlated with the fungus infection were quercetin-3-galactoside, quercetin-3-glucoside, kaempferol-3-galactoside and isorhamnetin-3-glucoside. Modulation of pathogen-induced phenolics also far from inoculation site might suggest a migration of signaling molecules from the infected area to healthy tissues.

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“…6). Expansin (EXP) contributes to cell wall disassembly through a non-enzymatic mechanism by disrupting non-covalent linkages at the cellulose/ hemicelluloses interface [49]. Hayama et al indicated that EXP2 is more likely to play a role in the expansion process in ripe fruit, and not related to the loss of firmness [50].…”

Section: Possible Differences Occurring Between Melting and Non-meltimentioning

confidence: 99%

Characteristics and regulatory pathway of the PrupeSEP1 SEPALLATA gene during ripening and softening in peach fruits

Qian

et al. 2017

Plant Science

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SEPALLATA genes are members of a subfamily of MADS-box transcription factors, and have essential roles in floral organ development and fruit ripening. In this study, the PrupeSEP1 gene was cloned from peach flesh. Its deduced amino acid sequence was very similar to that of MdMADS8 and MdMADS9 in apple and MADS-RIN-like in strawberry. During storage of melting flesh (MF) peach, the pattern of SEP1 expression was similar to that of ethylene biosynthesis and ethylene signal transduction-related gene expression (EIN2 and ETR2). The SEP1 expression level was correlated with that of EIN2 and ETR2. Furthermore, in MF, cell wall modification-related genes (Endo-PG3, EXP2 and PME3), N-glycan processing genes (β-Hex2 and α-Man) and Lox1 exhibited similar expression patterns to that of SEP1. However, in non-melting flesh (NMF) peach, the SEP1 expression pattern was different from that of MF peach. Moreover, in NMF, only EXP2, LOX1, and α-Man expression patterns were similar to that of SEP1. After SEP1 expression was inhibited by virus-induced gene silencing technique (VIGS) in MF peach, compared with the control, the fruit remained firm and fruit softening was delayed. While expression levels of the ripening and softening related genes, ACS2, EIN2, PME1, Endo-PG3, ACO1, ETR2,β-Hex2 and Lox1, were significantly decreased in SEP1-silenced peach, the transcription of EXP2 and PME3 were significantly enhanced except at the harvest stage. Yeast one-hybrid verification showed SEP1 can interact with promoter of target genes PGs. Our results indicate SEP1 may regulate fruit ripening and softening of MF peaches, while this regulation may be lost in the NMF peaches.

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Cell wall metabolism of peaches and nectarines treated with UV‐B radiation: a biochemical and molecular approach

Abstract: Based on these results, post-harvest UV-B treatment may be considered a promising tool to improve shelf-life and quality of peach fruit.

Cited by 12 publications

References 74 publications

Transcriptomic Analysis on the Peel of UV-B-Exposed Peach Fruit Reveals an Upregulation of Phenolic- and UVR8-Related Pathways

Transcriptomic Analysis on the Peel of UV-B-Exposed Peach Fruit Reveals an Upregulation of Phenolic- and UVR8-Related Pathways

UV-B Pre-treatment Alters Phenolics Response to Monilinia fructicola Infection in a Structure-Dependent Way in Peach Skin

Characteristics and regulatory pathway of the PrupeSEP1 SEPALLATA gene during ripening and softening in peach fruits

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