Cell Wall Mannan of Candida Attenuates Osteogenic Differentiation by Human Dental Pulp Cells

Bulanawichit, Wajathip; Nguyen, Thu Ngoc Yen; Ritprajak, Patcharee; Nowwarote, Nunthawan; Osathanon, Thanaphum

doi:10.1016/j.joen.2022.11.010

Cited by 1 publication

(2 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the contrary, other studies demonstrated that PG -LPS and E.coli LPS exhibited inhibitory effects on osteoblastic differentiation of human periodontal ligament stem cells and murine dental pulp-derived cell lines [ 21 , 26 , 29 , 30 ]. Further, exposure of dental pulp cells to the cell wall mannan of Candida species, a CLR ligand, led to the reduction of mineralisation [ 18 ]. In accordance with these latter studies, our result showed that different concentrations of TLR and CLR agonists used in the present study ( PG -LPS, E.coli LPS, poly(I:C), Pam3CSK4, Furfurman, and Zymosan) impaired the osteogenic differentiation of hDPSCs, as evidenced by the reduction in both ALP activity and mineralised nodule formation.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Toll-like receptor and C-type lectin receptor agonists attenuate osteogenic differentiation in human dental pulp stem cells

Bulanawichit,

Sinsareekul,

Kornsuthisopon

et al. 2024

BMC Oral Health

Self Cite

View full text Add to dashboard Cite

Background This study aimed to investigate the effects of various toll-like receptor (TLR) and C-type lectin receptor (CLR) ligands on osteogenic differentiation in human dental pulp stem cells (hDPSCs). Methods hDPSCs were cultured and treated with various concentrations (0.01, 0.1, 1.0, and 10 µg/mL) of TLR or CLR agonists (PG-LPS, E.coli LPS, poly(I:C), Pam3CSK4, Furfurman, and Zymosan). Cell viability was determined by MTT assay. The effects of TLR and CLR agonists on osteogenic differentiation of hDPSCs were measured by alkaline phosphatase (ALP) activity, Alizarin Red S staining, and Von Kossa staining. In addition, the mRNA expression of osteogenesis-related genes (ALP, COL1A1, RUNX2, OSX, OCN and DMP1) was examined by RT-qPCR. A non-parametric analysis was employed for the statistical analyses. The statistically significant difference was considered when p < 0.05. Results Treatment with TLR and CLR agonists was associated with an increase in hDPSCs’ colony-forming unit ability. Compared with the control group, TLR and CLR agonists significantly inhibited the osteogenic differentiation of hDPSCs by decreasing the ALP activity, mineralised nodule formation, and mRNA expression levels of osteogenesis-related genes (ALP, COL1A1, RUNX2, OSX, OCN and DMP1). The inhibition of TRIF but not Akt signalling rescued the effects of TLR and CLR agonist attenuating hDPSCs’ mineralisation. Conclusions The activation of TLRs or CLRs exhibited an inhibitory effect on osteogenic differentiation of hDPSCs via the TRIF-dependent signalling pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

“…From the result of previous studies, it can be implied that different microbial compositions (bacteria and fungi) of the caries lesion may exert distinct influences on the inflammatory response of the pulp [ 18 , 19 ]. However, there are some contradictory data regarding the roles of different TLRs in the differentiation of hDPSCs.…”

Section: Introductionmentioning

confidence: 99%