Cell Wall Localization of Two DUF642 Proteins, BIIDXI and TEEBE, during Meloidogyne incognita Early Inoculation

Salazar-Iribe, Alexis; Zúñiga-Sánchez, Esther; Mejía, Emma Zavaleta; Gamboa-deBuen, and Alicia

doi:10.5423/ppj.nt.05.2017.0101

“…Its product is a protein that contains a DUF642 domain and a galactose-binding-like domain fold, and it is member of a group of seven proteins in Eucalyptus that have orthology relationships to a group of ten proteins in Arabidopsis . Remarkably, two members of this group are AT4G32460 (BIIDXI locus) and AT2G41800 (TEEBE locus), recently reported as highly induced genes by auxin during early interaction between the susceptible A. thaliana ecotype Columbia and the RKN M. incognita [60]. SNP EuBR03s30383415 is also predicted to cause an allelic change in the region downstream of another gene of P. guajava , J3R85_001444, which has orthology relationship to Eucgr.C01790 (Uniprot id: A0A059CQ27) in Eucalyptus .…”

Section: Discussionmentioning

confidence: 99%

Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae

Santos

¹

,

Costa

²

,

Boiteux

³

et al. 2022

Preprint

0

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Tropical fruit tree species constitute a yet untapped supply of outstanding diversity of taste and nutritional value, barely developed from the genetics standpoint, with scarce or no genomic resources to tackle the challenges arising in modern breeding practice. We generated a de novo genome assembly of the Psidium guajava, the super fruit “apple of the tropics”, and successfully transferred 14,268 SNP probesets from Eucalyptus to Psidium at the nucleotide level, to detect genomic loci linked to resistance to the root knot nematode (RKN) Meloidogyne enterolobii derived from the wild relative P. guineense. Significantly associated loci with resistance across alternative analytical frameworks, were detected at two SNPs on chromosome 3 in a pseudo-assembly of Psidium guajava genome built using a syntenic path approach with the Eucalyptus grandis genome to determine the order and orientation of the contigs. The P. guineense-derived resistance response to RKN and disease onset is conceivably triggered by mineral nutrients and phytohormone homeostasis or signaling with the involvement of the miRNA pathway. Hotspots of mapped resistance quantitative trait loci and functional annotation in the same genomic region of Eucalyptus provide further indirect support to our results, highlighting the evolutionary conservation of genomes across genera of Myrtaceae in the adaptation to pathogens. Marker assisted introgression of the resistance loci mapped should accelerate the development of improved guava cultivars and hybrid rootstocks.

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“…thaliana ecotype Columbia and the RKN M . incognita [ 64 ]. SNP EuBR03s30383415 is also predicted to cause an allelic change in the region downstream of another gene of P .…”

Section: Discussionmentioning

confidence: 99%

“…Its product is a protein that contains a DUF642 domain and a galactose-binding-like domain fold, and it is member of a group of seven proteins in Eucalyptus that have orthology relationships to a group of ten proteins in Arabidopsis. Remarkably, two members of this group are AT4G32460 (BIIDXI locus) and AT2G41800 (TEEBE locus), recently reported as highly induced genes by auxin during early interaction between the susceptible A. thaliana ecotype Columbia and the RKN M. incognita [64]. SNP EuBR03s30383415 is also predicted to cause an allelic change in the region downstream of another gene of P. guajava, J3R85_001444, which has orthology relationship to Eucgr.C01790 (Uniprot id: A0A059CQ27) in Eucalyptus.…”

Section: Associated Snps Are Located Within or In Close Proximity To ...mentioning

confidence: 99%

Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae

Santos

¹

,

Costa²,

Boiteux³

et al. 2022

PLoS ONE

0

View full text Add to dashboard Cite

Tropical fruit tree species constitute a yet untapped supply of outstanding diversity of taste and nutritional value, barely developed from the genetics standpoint, with scarce or no genomic resources to tackle the challenges arising in modern breeding practice. We generated a de novo genome assembly of the Psidium guajava, the super fruit “apple of the tropics”, and successfully transferred 14,268 SNP probesets from Eucalyptus to Psidium at the nucleotide level, to detect genomic loci linked to resistance to the root knot nematode (RKN) Meloidogyne enterolobii derived from the wild relative P. guineense. Significantly associated loci with resistance across alternative analytical frameworks, were detected at two SNPs on chromosome 3 in a pseudo-assembly of Psidium guajava genome built using a syntenic path approach with the Eucalyptus grandis genome to determine the order and orientation of the contigs. The P. guineense-derived resistance response to RKN and disease onset is conceivably triggered by mineral nutrients and phytohormone homeostasis or signaling with the involvement of the miRNA pathway. Hotspots of mapped resistance quantitative trait loci and functional annotation in the same genomic region of Eucalyptus provide further indirect support to our results, highlighting the evolutionary conservation of genomes across genera of Myrtaceae in the adaptation to pathogens. Marker assisted introgression of the resistance loci mapped should accelerate the development of improved guava cultivars and hybrid rootstocks.

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Degree of pectin methyl esterification in endosperm cell walls is involved in embryo bending in Arabidopsis thaliana

Cruz-Valderrama

¹

,

Jiménez‐Durán

²

,

Zúñiga-Sánchez

³

et al. 2018

Biochemical and Biophysical Research Communications

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Cell Wall Localization of Two DUF642 Proteins, BIIDXI and TEEBE, during Meloidogyne incognita Early Inoculation

Cited by 7 publications

References 17 publications

Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae

Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae

Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae

Degree of pectin methyl esterification in endosperm cell walls is involved in embryo bending in Arabidopsis thaliana

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