Cell wall invertase as a regulator in determining sequential development of endosperm and embryo through glucose signaling early in seed development

Wang, Lu; Liao, Shengjin; Ruan, Yong‐Ling

doi:10.4161/psb.22722

Cited by 3 publications

(2 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Molecular genetic studies have shown that cell wall invertase (CWIN) proteins play a key role in determining the sequential development of the endosperm and the embryo 2 , and a positive correlation has been observed between CWIN activity and seed development in a range of plant species including bean ( Vicia faba ) 3 , 4 , barley ( Hordeum vulgare ) 5 , maize ( Zea mays ) 6 , rice ( Oryza sativa ) 7 , tomato ( Solanum lycopersicum ) 8 , cotton ( Gossypium hirsutum ) 9 , and soybean ( Glycine max ) 10 . Mutants of maize and rice with reduced CWIN expression produce smaller seeds 7 , 11 , whereas elevation of CWIN activity by silencing its inhibitor in tomato increases the seed size 12 .…”

Section: Introductionmentioning

confidence: 99%

Aberrant seed development in Litchi chinensis is associated with the impaired expression of cell wall invertase genes

Zhang

et al. 2018

Hortic Res

View full text Add to dashboard Cite

Cell wall invertase (CWIN) are known to play important roles in seed development. However, most reports to date have focused on a single gene family member, and have mainly investigated CWIN functions during the filling stage of seed development. In this study, we found significant lower levels of CWIN protein and activity associated with seed abortion in the Litchi chinensis cultivar “Nuomici.” We identified five litchi CWIN genes and observed that the expression of LcCWIN5 was limited to the flower tissues and decreased sharply with fruit development. Silencing of LcCWIN5 expression before 28 DAA (cell division stage) resulted in perturbed liquid endosperm development, smaller seeds, and higher seed abortion rate, while silencing after 28 DAA (filling stage) had no effect on seed development. In contrast, LcCWIN2 was mostly expressed in the funicle and seed coat, and increased with fruit development. Decreased LcCWIN2 expression and CWIN activity during early seed filling coincided with smaller seeds in the cultivar “Feizixiao.” Silencing of LcCWIN2 caused a reduction in the seed size without inducing seed abortion. We propose that CWIN activity in seed maternal tissues during cell division stage is likely due to LcCWIN5 expression, which regulates early seed development. On the other hand, CWIN activity during the filling stage is due to the expression of LcCWIN2, which may promote carbon import by creating a sucrose gradient. Comparable LcCWIN5 expression, but much lower CWIN activity, detected in the funicle of “Nuomici” is consistent with post-translational regulation.

show abstract

Section: Introductionmentioning

confidence: 99%

Aberrant seed development in Litchi chinensis is associated with the impaired expression of cell wall invertase genes

Zhang

et al. 2018

Hortic Res

View full text Add to dashboard Cite

show abstract

“…Since the substrates and products of this kind of enzyme are both nutrients and signal molecules, CWINVs participate in many aspects of plant development and growth (Roitsch and Gonzalez, 2004). These include sucrose and starch accumulation in carrot roots (Tang et al, 1999) and tomato fruit (Zhang N. et al, 2015); pathogenesis in tomato (Schaarschmidt et al, 2006; Kocal et al, 2008; Bonfig et al, 2010), tobacco (Essmann et al, 2008), and rice (Sun et al, 2014); and seed development in tomato (Jin et al, 2009), cotton (Wang and Ruan, 2012; Wang et al, 2013), maize, sorghum, and rice (Chourey et al, 2010; Jain et al, 2010; Li et al, 2013; French et al, 2014). These CWINVs might perform sucrose hydrolysis in various tissues, mainly in phloem, to facilitate phloem loading/unloading.…”

Section: Introductionmentioning

confidence: 99%

Cell Wall Invertase 3 Affects Cassava Productivity via Regulating Sugar Allocation From Source to Sink

Yan

et al. 2019

Front. Plant Sci.

View full text Add to dashboard Cite

Storage roots are the main sink for photo-assimilate accumulation and reflect cassava yield and productivity. Regulation of sugar partitioning from leaves to storage roots has not been elucidated. Cell wall invertases are involved in the hydrolysis of sugar during phloem unloading of vascular plants to control plant development and sink strength but have rarely been studied in root crops like cassava. MeCWINV3 encodes a typical cell wall invertase in cassava and is mainly expressed in vascular bundles. The gene is highly expressed in leaves, especially mature leaves, in response to diurnal rhythm. When MeCWINV3 was overexpressed in cassava, sugar export from leaves to storage roots was largely inhibited and sucrose hydrolysis in leaves was accelerated, leading to increased transient starch accumulation by blocking starch degradation and reduced overall plant growth. The progress of leaf senescence was promoted in the MeCWINV3 over-expressed cassava plants with increased expression of senescence-related genes. Storage root development was also delayed because of dramatically reduced sugar allocation from leaves. As a result, the transcriptional expression of starch biosynthetic genes such as small subunit ADP-glucose pyrophosphorylase , granule-bound starch synthase I , and starch branching enzyme I was reduced in accordance with insufficient sugar supply in the storage roots of the transgenic plants. These results show that MeCWINV3 regulates sugar allocation from source to sink and maintains sugar balance in cassava, thus affecting yield of cassava storage roots.

show abstract

Integration of Morphological, Physiological, Transcriptome and Proteome Analyses Reveal the Role and Mechanism of Photoassimilate Alterations in Modulating Early Seed Development of Torreya Grandis

Suo,

Zhong,

et al. 2023

Preprint

View full text Add to dashboard Cite

Cell wall invertase as a regulator in determining sequential development of endosperm and embryo through glucose signaling early in seed development

Cited by 3 publications

References 20 publications

Aberrant seed development in Litchi chinensis is associated with the impaired expression of cell wall invertase genes

Aberrant seed development in Litchi chinensis is associated with the impaired expression of cell wall invertase genes

Cell Wall Invertase 3 Affects Cassava Productivity via Regulating Sugar Allocation From Source to Sink

Integration of Morphological, Physiological, Transcriptome and Proteome Analyses Reveal the Role and Mechanism of Photoassimilate Alterations in Modulating Early Seed Development of Torreya Grandis

Contact Info

Product

Resources

About