Cell wall glycopeptides of Candida albicans serotypes A and B

Kolarova, N.; Masler, L.; Šikl, D.

doi:10.1016/0005-2795(73)90348-6

Cited by 21 publications

(8 citation statements)

References 16 publications

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“…We used sucrose in the metabolic labeling media and glucose in the growth media for cells labeled with 1251I. It is interesting that the mannoprotein we detected was metabolically labeled with L-[35S]rnethionine, since all determinations of amino acid composition of mannoprotein isolated by chemical methods from cell walls of C. albicans or S. cerevisiae have noted the absence of sulfur-containing amino acids (7,11,12). The reasons for this are unknown, but perhaps such amino acids are destroyed or lost by the extraction procedures used.…”

Section: Discussionmentioning

confidence: 99%

Antigenic differences between mannoproteins of germ tubes and blastospores of Candida albicans

Sundstrom¹,

Nichols²,

Kenny³

1987

Infect Immun

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To determine the nature of germ tube-specific antigens of Candida albicans, procedures for intrinsically labeling cell wall antigens metabolically were developed. Blastospores or germ tubes labeled either in their proteins with L-[35S]methionine or in mannose-containing carbohydrates with D[2-3H]mannose contained surface components similar to those found previously with 125I-labeled organisms. Germ tube-specific determinants, were found on a 200-kilodalton protein in digests from germ tubes, whereas a component of similar molecular size in blastospore extracts reacted weakly or not at all with germ tube-specific antibody. In addition, a glycan fraction prepared from germ tubes reacted with the unadsorbed anti-C. albicans polyvalent antibody but not with the germ tube-specific antibody, suggesting that the germ tube-specific determinants are not carbohydrates.

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Section: Discussionmentioning

confidence: 99%

Antigenic differences between mannoproteins of germ tubes and blastospores of Candida albicans

Sundstrom¹,

Nichols²,

Kenny³

1987

Infect Immun

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“…In this respect no differences in the major cytoplasmic proteins of any of the yeast or mycelial phase extracts have been identified (Manning & Mitchell, 1980). In addition, the overall wall composition of the two cellular forms has been shown to be similar (Chattaway et al, 1968), but the relative amounts of alkali-soluble and alkali-insoluble glucans (Sullivan et al, 1983) as well as the glycopeptides extracted with ethylenediamine (Kolarova et al, 1973) have been reported to change during germ-tube formation. Elorza et al (1983) showed that walls of yeast cells contain more mannan than those of the mycelial phase, suggesting that the incorporation of specific mannoproteins might be responsible for the different wall architecture.…”

Section: Discussionmentioning

confidence: 99%

Dimorphism in Candida albicans: Contribution of Mannoproteins to the Architecture of Yeast and Mycelial Cell Walls

1985

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Wall mannoproteins of the two (yeast and mycelial) cellular forms of Candida albicans were solubilized by different agents. Boiling in 2% (w/v) SDS was the best method, as more than 70% of the total mannoprotein was extracted. Over 40 different bands (from 15 to 80 kDal) were detected on SDS-polyacrylamide gel electrophoresis of this material. The residual wall mannoproteins were released after enzymic (Zymolyase and endogenous wall P-glucanases) degradation of wall glucan, suggesting that they are covalently linked to this structural polymer. Four bands (of 160 kDal, 205 kDal and higher molecular mass) were observed in the material released from yeast walls but only the two smaller components were detected in the material obtained from mycelial walls. Moreover, the mannoproteins of high molecular mass, which are covalently linked in walls of normal cells, were not incorporated into walls of regenerating protoplasts, but non-covalently linked mannoproteins were retained from the beginning of the process.

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“…The mannan and/3-glucan are complexed with proteins, which in the yeast phase are present as water-soluble, and water-insoluble glycopeptides, both forms composed of mannan peptides and glucan peptides (84). The ratio protein/carbohydrate of both types of water-soluble glycopeptides was higher in serotype A than in serotype B. Milder methods of extraction resulted in isolation of a series of three complex molecules, all of them with antigenic properties: a) peptidoglucomannan with a glucose: mannose ratio of 2:3, and an aminoacid content of 7.3%; b) a soluble mannoglucan with 11% mannose.…”

Section: Candida Albicansmentioning

confidence: 99%

The cell wall of fungal human pathogens: Its possible role in host-parasite relationshipsA review

San-Blas

1982

Mycopathologia

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The ability of some fungi to cause disease in animals, and particularly in humans, appears to be related to some peculiar trait of their metabolism not shared by taxonomically related species, so that among thousands of species of fungi, only some 100 are considered pathogenic for humans.Recently, there has been an increase in the basic research aimed at elucidating the physiology, biochemistry, and mechanisms of pathogenicity of the fungi in addition to the responses of the infected host. The results from these studies are often difficult to compare because of the lack of reference cultures, and because variability among laboratories are found for many parameters such as origin of isolates, growth conditions, and fractionation procedures used to obtain chemically defined components of cell walls.This review deals with cell wall structures and their influence in host-parasite relationships in those relatively few pathogenic fungi on which substantial literature exists. Serological properties of the walls are included when available. Readers interested in medical and biological aspects of fungi may consult Rippon's (140) and Emmons et al.'s (51) textbooks, among others. Some reviews on microbial surfaces and their relationships to pathogenicity have been published (160 162)2The pathogenic fungi included in this review are classified according to their cell wall structure, following Bartnicki-Garcia's criteria (15) on the distribution of the two major polysaccharides within the fungal cell wall. This classification is based on the fact that fungi may be subdivided into various categories according to the chemical nature of their walls, and that these categories closely parallel conventional taxonomic boundaries. As seen in Table l, this classification is based on dual combinations of those polysaccharides which appear to be the principal component of vegetative walls, disregarding the presence of small amounts of other classifying polysaccharides. The few pathogenic fungi whose cell walls have been studied to some extent belong to Bartnicki-Garcia's categories 5 (chitin-glucan) and 6 (mannan-glucan) ( Table 2). Besides these main polysaccharides, some minor components of the cell wall may also play important roles in pathogenicity, as antigens, or as virulence factors, and they will be mentioned in this review. DermatophytesDermatophytes are a highly specialized group of fungi that, through long evolutionary processes became adapted to invade, colonize, and nourish themselves on the keratinized tissues of animals. The three genera of dermatophytes are: Trichophyton, Microsporum, and Epidermophyton, of which 21, 15, and 1 species, respectively, are currently considered valid members (1) (ln the Vth Panamerican Conference on Mycoses, April 1980, Caracas, these figures were raised to 27, 16, and 2, respectively). Mating studies have revealed that, for some cases, what were considered as single species of Microsporum and Trichophyton, actually represent complexes of more than one species, indis- Mycopathologia7...

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Cell wall glycopeptides of Candida albicans serotypes A and B

Cited by 21 publications

References 16 publications

Antigenic differences between mannoproteins of germ tubes and blastospores of Candida albicans

Antigenic differences between mannoproteins of germ tubes and blastospores of Candida albicans

Dimorphism in Candida albicans: Contribution of Mannoproteins to the Architecture of Yeast and Mycelial Cell Walls

The cell wall of fungal human pathogens: Its possible role in host-parasite relationshipsA review

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