Cell Wall Expansion as Viewed by the Creep Method

Suslov, Dmitry; Vissenberg, Kris

doi:10.1007/978-3-319-79099-2_14

Cited by 4 publications

(2 citation statements)

References 68 publications

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“…Arabidopsis seedlings for creep tests were placed individually into 1.5-mL Eppendorf test tubes, frozen by immersing the closed tubes into liquid nitrogen, stored at −20 °C and used for biomechanical analyses within 2 weeks after freezing. In vitro extension of frozen/thawed hypocotyls was measured with a custom-built constant-load extensometer [ 47 , 48 ]. A 5-mm-long basal segment (located 1.5 mm above the root neck) of a five-day-old hypocotyl or a 3-mm-long subapical segment (located 1.5 mm below the apical hook) of a 76(78)-hour-old hypocotyl was secured between clamps of an extensometer and preincubated in a buffer (20 mM sodium acetate, pH 5.0, or 20 mM MES-KOH, pH 6.0) in a relaxed state for 2 min.…”

Section: Methodsmentioning

confidence: 99%

Sucrose and Mannans Affect Arabidopsis Shoot Gravitropism at the Cell Wall Level

Pozhvanov,

Suslov

2024

Plants

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Gravitropism is the plant organ bending in response to gravity. Gravitropism, phototropism and sufficient mechanical strength define the optimal position of young shoots for photosynthesis. Etiolated wild-type Arabidopsis seedlings grown horizontally in the presence of sucrose had a lot more upright hypocotyls than seedlings grown without sucrose. We studied the mechanism of this effect at the level of cell wall biomechanics and biochemistry. Sucrose strengthened the bases of hypocotyls and decreased the content of mannans in their cell walls. As sucrose is known to increase the gravitropic bending of hypocotyls, and mannans have recently been shown to interfere with this process, we examined if the effect of sucrose on shoot gravitropism could be partially mediated by mannans. We compared cell wall biomechanics and metabolomics of hypocotyls at the early steps of gravitropic bending in Col-0 plants grown with sucrose and mannan-deficient mutant seedlings. Sucrose and mannans affected gravitropic bending via different mechanisms. Sucrose exerted its effect through cell wall-loosening proteins, while mannans changed the walls’ viscoelasticity. Our data highlight the complexity of shoot gravitropism control at the cell wall level.

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Section: Methodsmentioning

confidence: 99%

Sucrose and Mannans Affect Arabidopsis Shoot Gravitropism at the Cell Wall Level

Pozhvanov,

Suslov

2024

Plants

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“…It should be noted that creep measurements in general are carried out on isolated and often heat-inactivated fragments from cucumber or Arabidopsis hypocotyls, mungbean epicotyls, oat coleoptiles or the above mentioned onion peels (Suslov and Vissenberg 2018). The cells in these tissues are unique in that cell expansion occurs uncoupled from cell wall deposition, perhaps as an adaptation allowing rapid growth.…”

Section: Chemorheological Cell Wall Expansionmentioning

confidence: 99%

Cell Wall-Related Mechanisms Underlying Plant Cell Expansion

Vissenberg,

Höfte

2023

Plant Cell Walls

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A central question in plant biology is how plant cells can expand while maintaining a strong cell wall. Two main mechanisms have been proposed for irreversible wall expansion, intussusception and chemorheological growth. We discuss here the molecular nature of the cross-links and how their removal can contribute to growth of both tip-growing or diffusely-growing cells. We also discuss recent observations suggesting that at least in some cases processive intussusception may contribute to cell wall expansion in plants.

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Cell wall dynamics: novel tools and research questions

Baez

Bacete

2023

Journal of Experimental Botany

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Years ago, a classic textbook would define plant cell walls based on passive features. For instance, a sort of plant exoskeleton of invariable polysaccharide composition, and probably painted in green. However, currently, this view has been expanded to consider plant cell walls as active, heterogeneous, and dynamic structures with a high degree of complexity. However, what do we mean when we refer to a cell wall as a dynamic structure? How can we investigate the different implications of this dynamism? While the first question has been the subject of several recent publications, defining the ideal strategies and tools needed to address the second question has proven to be challenging due to the myriad of techniques available. In this review, we will describe the capacities of several methodologies to study cell wall composition, structure, and other aspects developed or optimized in recent years. Keeping in mind cell wall dynamism and plasticity, the advantages of performing long-term non-invasive live-imaging methods will be emphasized. We specifically focus on techniques developed for Arabidopsis thaliana primary cell walls, but the techniques could be applied to both secondary cell walls as well as other plant species. We believe this toolset will help researchers in expanding knowledge of these dynamic/evolving structures.

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Cell Wall Expansion as Viewed by the Creep Method

Cited by 4 publications

References 68 publications

Sucrose and Mannans Affect Arabidopsis Shoot Gravitropism at the Cell Wall Level

Sucrose and Mannans Affect Arabidopsis Shoot Gravitropism at the Cell Wall Level

Cell Wall-Related Mechanisms Underlying Plant Cell Expansion

Cell wall dynamics: novel tools and research questions

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