Cell wall degrading enzymes of vascular wilt fungi. III. Possible involvement of endo-pectin lyase in Verticillium wilt of tomato

Cooper, Richard M.; Wood, R. K. S.

doi:10.1016/0048-4059(80)90043-0

Cited by 58 publications

(32 citation statements)

References 47 publications

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“…Support for this speculation came from observations that altered cell walls in highly colonized plant tissues failed to stain with ferric hydroxylamine or ruthenium red, considered to be pectin specific (4). Other lines of indirect evidence were derived from induction of wilt symptoms in plant cuttings by preparations containing pectic-degrading enzymes (19), and from pathogenicity correlated to enzyme production (16). In this context, particular attention has been paid to pectolytic enzymes produced by Fusarium oxysporum f. sp.…”

mentioning

confidence: 99%

“…In this context, a central role is played by chain-splitting pectic enzymes which cause disruption of pectin layers, thus allowing further intercellular growth of the pathogen (18,19). It is now well documented that pectic polymers are enzymatically vulnerable ( 15) and that, in turn, their degradation can have diverse effects on the outcome of host-pathogen interactions.…”

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confidence: 99%

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Implication of Pectic Components in Cell Surface Interactions between Tomato Root Cells and Fusarium oxysporum f. sp. radicis-lycopersici

Benhamou¹,

Chamberland²,

Pauzé³

1990

Plant Physiol.

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Aplysia gonad lectin, a polygalacturonic acid-binding lectin isolated from the sea mollusc Aplysia depilans, was complexed to colloidal gold and used for localizing polygalacturonic-acidcontaining molecules in tomato root tissues infected with Fusarium oxysporum f. sp. radicis-lycopersici (FORL). Colonization of host tissues by FORL was associated with striking wall modifications including disruption and even loss of middle lamellae. According to the labeling pattern observed in host wall areas adjacent to fungal penetration channels, it is likely that FORL pectolytic enzymes act through localized wall degradation. The release of polygalacturonic acid-rich wall fragments and the accumulation of polygalacturonic acid-containing molecules in some altered phloem cells were frequently observed and considered to be specific host reactions to fungal attack. The heavy deposition of such molecules at strategic sites such as wall oppositions and intercellular spaces provides support to their implication in the plant defense system. The possible interrelation between polygalacturonic acid-containing molecules and other polymers such as lignin and phenolic compounds remains to be investigated further. The role of these molecules in host-pathogen interactions is discussed in relation to plant defense.

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confidence: 99%

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confidence: 99%

Implication of Pectic Components in Cell Surface Interactions between Tomato Root Cells and Fusarium oxysporum f. sp. radicis-lycopersici

Benhamou¹,

Chamberland²,

Pauzé³

1990

Plant Physiol.

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“…These enzymes are involved in degradation of plant cell components and lignin. Pectic enzymes have been frequently associated with wilt disease on various crops (Cooper and Wood, 1980). Pectic enzymes consist primarily of pectin methyl esterase, polygalacturonase and pectate lyase (Kawano et al, 1999;Verlent et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Combinatorial efficacy of Trichoderma spp. and Pseudomonas fluorescens to enhance suppression of cell wall degrading enzymes produced by Fusarium wilt of Arachis hypogaea.L

Rajeswari

Kapoor

2018

Int. J. Agril. Res. Innov. & Tech.

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Fusarium oxysporum, the soil borne pathogen causes vascular wilt, on majority of crop plants. It has been demonstrated that two different species of Trichoderma and Pseudomonas fluorescens suppress disease by different mechanisms. Therefore, application of a mixture of these biocontrol agents, and thus of several suppressive mechanisms, may represent a viable control strategy. A necessity for biocontrol by combinations of biocontrol agents can be the compatibility of the co-inoculated micro-organisms. Hence, compatibility between Trichoderma spp. and Pseudomonas fluorescens that have the ability to suppress Fusarium oxysporum in vitro on the activity of pectinolytic enzymes of Fusarium oxysporum. The activity of pectinolytic enzymes, i.e. pectin methyl esterase, endo and exo polymethylgalacturonases and exo and endo pectin trans eliminases produced by Fusarium oxysporum (Control) was higher. Maximum inhibition of pectin methylesterase, exo and endo polymethylgalacturonase and exo and endopectin trans eliminase was shown by culture filtrate of Trichoderma viride + Pseudomonas fluorescens (Tv+Pf) (1+2%), followed by Trichoderma harzianum + Pseudomonas fluorescens, (Th +Pf) (1.5+2%) and Trichoderma viride + Trichoderma harzianum (Tv+Th) (1+1.5%). However, pathogenecity suppression of Fusarium oxysporum, a causative of Arachis hypogaea. L by the compatible combination of Trichodema viride + Pseudomonas fluorescens (1+2%) was significantly better as compared to the single bio-agent. This indicates that specific interactions between biocontrol agents influence suppression of pathogenicity factors directly by combinations of these compatible bio-agents.

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“…Several reasons such as secretion in very low concentrations, ionic binding to negatively charged cell walls and vascular elements (Cooper, 1983;Cooper & Wood, 1980;Keon et al, 1987), and inhibition by plant proteins during extraction (Ferraris et al, 1996) have been proposed as possible explanations of this phenomenon. Thus, the nondetection of PG in infected tissues does not rule out its involvement in the development of infection.…”

Section: Discussionmentioning

confidence: 99%

Effect of rubber petiole cell walls and virulence of strains on secretion of pectic enzymes by <i>Pytophthora meadii</i>

Jayasuriya

Wijesundera

Thennakoon

2009

Ceylon J. Sci. (Biol. Sci.)

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The characteristics of the pectic enzyme polygalacturonase (PG) of Phytophthora meadii isolates from petioles of rubber (Hevea brasiliensis) genotypes were compared in relation to (i) aggressiveness (virulence) in infecting detached petioles of a susceptible rubber genotype (PB86) or (ii) in relation to the availability of purified rubber petiole cell walls (obtained from a Phytophthora-resistant clone, RRIC100) in the growth medium, as the sole carbon source. The four most highly aggressive isolates produced PG forms varying within mw 48-60 kDa, while, the highly aggressive MAD86 isolate produced two PG forms of mw 48 and 70 kDa. Moderately or weakly aggressive isolates produced PG forms within the range of 62-66 kDa. With rubber petiole cell walls in the growth medium, the four most highly aggressive isolates produced PG forms of mw 42-62 kDa, while, MAD86 produced a much smaller PG form of 21 kDa. Weak or moderately aggressive isolates produced similar PG forms in both cell wall and pectin media. Quite smaller PG molecules produced in rubber cell wall medium could be due to elicitor molecular fragments bound to rubber cell walls, which may induce P. meadii to secret specific PG forms via signal transduction pathway. None of the isolates produced pectin lyase (PL) in any growth medium. Neither PG nor PL activity was detected in P. meadii-infected petioles.

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Cell wall degrading enzymes of vascular wilt fungi. III. Possible involvement of endo-pectin lyase in Verticillium wilt of tomato

Cited by 58 publications

References 47 publications

Implication of Pectic Components in Cell Surface Interactions between Tomato Root Cells and Fusarium oxysporum f. sp. radicis-lycopersici

Implication of Pectic Components in Cell Surface Interactions between Tomato Root Cells and Fusarium oxysporum f. sp. radicis-lycopersici

Combinatorial efficacy of Trichoderma spp. and Pseudomonas fluorescens to enhance suppression of cell wall degrading enzymes produced by Fusarium wilt of Arachis hypogaea.L

Effect of rubber petiole cell walls and virulence of strains on secretion of pectic enzymes by <i>Pytophthora meadii</i>

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