Cell wall degrading enzymes for silage. 2. Aerobic stability of enzyme‐treated laboratory silages

Abstract

The effects of two commercial cellulase/hemicellulase enzymes derived from Trichoderma reesei on silage fermentation and aerobic stability were investigated in three laboratory-scale experiments. In Experiment 1, perennial ryegrass (Lolium perenne) was treated wiih enzyme A at the rates of 0. 0-125, 0-250, 0-500 and 0-750 cm^ kg"'. In Experiment 2, Italian ryegrass (Lolium muhiflorum) was treated with the same enzyme at the rates of 0, 0-250 and 0-500 cm-' kg""' and with 85% formic acid {3-5 cm^ kg-'). In Experiment 3, perennial ryegrass was ensiled untreated, with enzyme A (0-250 and 0-500 cm-"* kg ') and with 0-200 and 0-400 cm^ kg"' enzyme B which also contained glucose oxidase. All silages were well preserved. Aerobic deterioration was related to numbers of lactate assimilating yeasts. In Experiment 1, all silages were stable over a 4-day period. In Experiment 2, enzyme A treatment delayed the temperature rise compared with the untreated control (P<0-05), whereas the formic acid-treated silage was stable. In Experiment 3, the temperature rise in the silage treated with the higher level of enzyme B occurred one day later than in the other silages (P<0-05).

The effect of enzyme treatment on the nutritive value of lucerne for equids

“…Therefore, such effects may be envisaged for enzymetreated silage fed to horses. However, although studies have shown improved fermentation characteristics and in vivo digestibilities of enzyme-treated silages in ruminants, a reduction in the aerobic stability of these silages has also been reported (Selmer-Olsen et al, 1993;Weinberg et al, 1995). This may be of particular interest to horse owners since the feeding-out of big bale silage is generally extended over a period of days.…”

Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing

“…Studies have shown that treatment with enzymes (cellulase, amylase, and pectinase) can enhance lactate levels and decrease ammonia-N levels by 40% (Sheperd et al 1995). A study by Selmer-Olsen et al (1993) showed that cellulase/hemicellulase enzymes improve the silage quality of perennial ryegrass (Lolium perenne) and Italian ryegrass (Lolium multi orum) and delay aerobic deterioration.…”

Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing

“…Studies have shown that treatment with enzymes (cellulase, amylase, and pectinase) can increase lactate levels and decrease ammonia-N levels by 40% (Sheperd et al 1995). A study by Selmer-Olsen et al (1993) showed that cellulase/hemicellulase enzymes improve the silage quality of perennial ryegrass (Lolium perenne) and Italian ryegrass (Lolium multi orum) and delay aerobic deterioration.…”