Cell wall damage and oxidative stress in Candida albicans ATCC10231 and Aspergillus niger caused by palladium nanoparticles

Athie-García, Martha Samira; Piñón-Castillo, Hilda Amelia; Muñoz-Castellanos, Laila Nayzzel; Ulloa-Ogaz, Ana Laura; Martínez-Varela, Perla Ivonne; Quintero‐Ramos, Armando; Duran, Robert; Murillo-Ramírez, J. G.; Orrantia‐Borunda, Erasmo

doi:10.1016/j.tiv.2018.01.006

Cited by 24 publications

(17 citation statements)

References 67 publications

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“…Previously, we have developed PdNPs and tested their effects as a fungicide, showing that treatments induce cell wall damage and oxidative stress in Aspergillus niger and Candida albicans [18]. Here, we extend these initial findings in fungus; we functionalized PdNPs with PVP (PdNPs-PVP) and with quercetin (PdNPs-PVP-Q).…”

Section: Introductionmentioning

confidence: 57%

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Palladium Nanoparticles Functionalized with PVP-Quercetin Inhibits Cell Proliferation and Activates Apoptosis in Colorectal Cancer Cells

et al. 2021

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Nanotechnology is focused on the development and application of novel nanomaterials with particular physicochemical properties. Palladium nanoparticles (PdNPs) have been used as antimicrobials, antifungals, and photochemicals and for catalytic activity in dye reduction. In the present investigation, we developed and characterized PdNPs as a carrier of quercetin and initiated a study of its effects in colorectal cancer cells. PdNPs were first functionalized with polyvinylpyrrolidone (PVP) and then coupled to quercetin (PdNPs-PVP-Q). Our results showed that quercetin was efficiently incorporated to PdNPs-PVP, as demonstrated using UV/Vis and FT-IR spectroscopy. Using transmission electron microscopy, we demonstrated a reduction in size from 3–14.47 nm of PdNPs alone to 1.8–7.4 nm of PdNPs-PVP and to 2.12–3.14 of PdNPs-PVP-Q, indicating an increase in superficial area in functionalized PdNPs-Q. Moreover, hydrodynamic size studies using dynamic light scattering showed a reduction in size from 2120.33 nm ± 112.53 with PdNPs alone to 129.96 nm ± 6.23 for PdNPs-PVP-Q, suggesting a major reactivity when quercetin is coupled to nanoparticles. X-ray diffraction assays show that the addition of PVP or quercetin to PdNPs does not influence the crystallinity state. Catalytic activity assays of PdNPs-PVP-Q evidenced the chemical reduction of 4-nitrophenol, methyl orange, and methyl blue, thus confirming an electron acceptor capacity of nanoparticles. Finally, biological activity studies using MTT assays showed a significant inhibition (p < 0.05) of cell proliferation of HCT-15 colorectal cancer cells exposed to PdNPs-PVP-Q in comparison to untreated cells. Moreover, treatment with PdNPs-PVP-Q resulted in the apoptosis activation of HCT-15 cells. In conclusion, here we show for the first time the development of PdNPs-PVP-Q and evidence its biological activities through the inhibition of cell proliferation and apoptosis activation in colorectal cancer cells in vitro.

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Section: Introductionmentioning

confidence: 57%

“…Phan et al [16] synthesized PdNP coatings with chitosan, which showed a 20% inhibition in MDA-MB-231 cells when irradiated with infrared light. Moreover, PdNPs have been used for bacterial [17] or fungal inhibition [18], showing effective inhibition. Different PdNP synthesis methods have been validated.…”

Section: Introductionmentioning

confidence: 99%

Palladium Nanoparticles Functionalized with PVP-Quercetin Inhibits Cell Proliferation and Activates Apoptosis in Colorectal Cancer Cells

et al. 2021

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“…Chemical reduction of palladium salt has produced PdNPs in an average size of 9 nm (±3.9 nm). The PdNPs showed a significative Candida albicans growth reduction at 150 ppm [107].…”

Section: Other Nanoparticlesmentioning

confidence: 95%

“…The squalene monooxygenase expression in Candida albicans exposed to TeNPs showed an increase [112]. The squalene monooxygenase catalyzes the conversion of squalene to 2,3-oxidosqualene; inhibition in this step generates the squalene increase and the disruption of the Candida albicans cell wall [107].…”

Section: Nanoparticle Characteristics and Toxicity And Candida Albicamentioning

confidence: 97%

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Nanoparticles as New Therapeutic Agents against Candida albicans

Castillo¹,

Muñoz-Castellanos²,

Chamorro³

et al. 2019

Candida Albicans

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Candida albicans is an opportunistic dimorphic yeast. This organism is pathogen associated to superficial and systemic infections. Actually, Candida albicans represents an emergent pathogen especially in a patient with some immunity compromises. Added to this, the use of antifungal in an indiscriminate form has increased the resistance of the existing drugs. In this aspect, the nanotechnology generates the possibility of creating new therapeutic agents. Nanoparticles are structures of 1-100 nm with special physicochemical characteristics that allow it to function as therapeutic agents or as carriers of these. Palladium, silver, and gold metallic nanoparticles and iron, titanium, zinc, and copper oxides have been used as growth inhibitors. These nanoparticles have been proved alone or in form of nanocomposites. The objective of this chapter is to describe the state of the art of the use of nanoparticles as inhibitors of the growth of Candida albicans, as well as the most relevant results regarding the mechanisms involved in this inhibition.

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