Cell wall composition and transcriptomics in stem tissues of stinging nettle (<i>Urtica dioica</i> L.): Spotlight on a neglected fibre crop

Xuan, Xuenan; Backes, Aurélie; Legay, Sylvain; Berni, Roberto; Faleri, Claudia; Gatti, Edoardo; Hausman, Jean-François; Cai, Giampiero; Guerriero, Gea

doi:10.1002/pld3.151

Cited by 17 publications

(45 citation statements)

References 120 publications

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“…The activation of sugar transporters may be critical for carbohydrate turnover, balancing of assimilates, and maintenance of osmotic status, all of which accompany TCW deposition in bast fibers. The analysis of cell wall composition and transcriptomics in stem tissues of stinging nettle (Urtica dioica L.) revealed up-regulation of SWEET15 gene in the cortical tissues sampled at the bottom; it was proposed that product of this gene could be involved in the transfer of sucrose to the thickening bast fibers (Xu et al, 2019).…”

Section: Genes Whose Expression Levels Are Highly Related To the Fibementioning

confidence: 99%

The Toolbox for Fiber Flax Breeding: A Pipeline From Gene Expression to Fiber Quality

et al. 2020

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Section: Genes Whose Expression Levels Are Highly Related To the Fibementioning

confidence: 99%

The Toolbox for Fiber Flax Breeding: A Pipeline From Gene Expression to Fiber Quality

et al. 2020

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“…In this study, the recently published de novo transcriptome of stinging nettle [24] was mined for CalS genes to study the expression in different tissues and internodes of the stem. Nettle is a bast fibre-producing plant and its cellulosic fibres are characterized by noteworthy lengths that are reached by a mechanism known as intrusive or invasive growth [25,26].…”

Section: Of 14mentioning

confidence: 99%

“…Nettle plants (clone 13) were grown as previously described [24,49,50]. To summarize, stem apical cuttings were taken from a donor plant, they were immediately dipped in tap water and subsequently in a commercial root-inducing hormone mix in powder form.…”

Section: Plant Growthmentioning

confidence: 99%

“…RNA was extracted and its purity/concentration checked as previously described [24,49,50]. Primers were designed with Primer3Plus ( [51] and available at: http://www.bioinformatics.nl/cgibin/primer3plus/primer3plus.cgi) and checked with the OligoAnalyzer Tool from Integrated DNA Technologies (IDT) (available at: https://eu.idtdna.com/pages/tools/oligoanalyzer).…”

Section: Rna Extraction and Gene Expression Analysismentioning

confidence: 99%

“…Primers were designed with Primer3Plus ( [51] and available at: http://www.bioinformatics.nl/cgibin/primer3plus/primer3plus.cgi) and checked with the OligoAnalyzer Tool from Integrated DNA Technologies (IDT) (available at: https://eu.idtdna.com/pages/tools/oligoanalyzer). Their amplification efficiencies were calculated using serial dilutions, as described previously [24,49,50].…”

Section: Rna Extraction and Gene Expression Analysismentioning

confidence: 99%

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Identification of Callose Synthases in Stinging Nettle and Analysis of Their Expression in Different Tissues

Guerriero

Piasecki

Berni

et al. 2020

IJMS

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Callose is an important biopolymer of β-1,3-linked glucose units involved in different phases of plant development, reproduction and response to external stimuli. It is synthesized by glycosyltransferases (GTs) known as callose synthases (CalS) belonging to family 48 in the Carbohydrate-Active enZymes (CAZymes) database. These GTs are anchored to the plasma membrane via transmembrane domains. Several genes encoding CalS have been characterized in higher plants with 12 reported in the model organism Arabidopsis thaliana. Recently, the de novo transcriptome of a fibre-producing clone of stinging nettle (Urtica dioica L.) was published and here it is mined for CalS genes with the aim of identifying members differentially expressed in the core and cortical tissues of the stem. The goal is to understand whether specific CalS genes are associated with distinct developmental stages of the stem internodes (elongation, thickening). Nine genes, eight of which encoding full-length CalS, are identified in stinging nettle. The phylogenetic analysis with CalS proteins from other fibre crops, namely textile hemp and flax, reveals grouping into 6 clades. The expression profiles in nettle tissues (roots, leaves, stem internodes sampled at different heights) reveal differences that are most noteworthy in roots vs. leaves. Two CalS are differentially expressed in the internodes sampled at the top and middle of the stem. Implications of their role in nettle stem tissue development are discussed.

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Immunohistochemical analyses on two distinct internodes of stinging nettle show different distribution of polysaccharides and proteins in the cell walls of bast fibers

Faleri

Mareri

et al. 2021

Protoplasma

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Stinging nettle is a perennial herbaceous species holding value as a multi-purpose plant. Indeed, its leaves and roots are phytofactories providing functional ingredients of medicinal interest and its stems produce silky and resistant extraxylary fibers (a.k.a. bast fibers) valued in the biocomposite sector. Similarly to what is reported in other fiber crops, the stem of nettle contains both lignified and hypolignified fibers in the core and cortex, respectively, and it is therefore a useful model for cell wall research. Indeed, data on nettle stem tissues can be compared to those obtained in other models, such as hemp and flax, to support hypotheses on the differentiation and development of bast fibers. The suitability of the nettle stem as model for cell wall-related research was already validated using a transcriptomics and biochemical approach focused on internodes at different developmental stages sampled at the top, middle, and bottom of the stem. We here sought to complement and enrich these data by providing immunohistochemical and ultrastructural details on young and older stem internodes. Antibodies recognizing non-cellulosic polysaccharides (galactans, arabinans, rhamnogalacturonans) and arabinogalactan proteins were here investigated with the goal of understanding whether their distribution changes in the stem tissues in relation to the bast fiber and vascular tissue development. The results obtained indicate that the occurrence and distribution of cell wall polysaccharides and proteins differ between young and older internodes and that these changes are particularly evident in the bast fibers.

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Cell wall composition and transcriptomics in stem tissues of stinging nettle (Urtica dioica L.): Spotlight on a neglected fibre crop

Cited by 17 publications

References 120 publications

The Toolbox for Fiber Flax Breeding: A Pipeline From Gene Expression to Fiber Quality

The Toolbox for Fiber Flax Breeding: A Pipeline From Gene Expression to Fiber Quality

Identification of Callose Synthases in Stinging Nettle and Analysis of Their Expression in Different Tissues

Immunohistochemical analyses on two distinct internodes of stinging nettle show different distribution of polysaccharides and proteins in the cell walls of bast fibers

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