Cell wall‐bound trans‐ and cis‐ferulic acids in growing maize roots

Abstract

E. 1994. Cell wall-bound trans-and cis-ferulic acids in growing maize roots -Physiol. Plant. 90; 734-738.The levels of ceil wall-bound trans-and ci's-ferulic acids in roots of dark grown Zea mays cv.LGl 1 plants were measured. They were quantified after alkaline hydrolysis of purified cell walls by reversed phase HPLC using fran.!-cinnamic acid as internal standard. The total amount of ferulic acid (trans-and cH-feruilc acid) in the root ba.se was 3-4 times higher than in the root tip. Cu-ferulic acid represented between 2% (tip) and 18% (base) of the total ferulic acid content. The total content of trans-and f is-femlic acids was approximately the same in the stele and the cortex, but the level of CK-ferulic acid in the stele was 5-6 times higher than in the cortex. Trans-and cK-ferulic acid levels as well as the percentage of cis-ferulic acid in the elongation zone were steady between 48 and 96 h after the beginning of germination. Slowly growing roots contained more wall-hound ferulic acids, particularly m-femlic acid, than fast growing roots. This relationship was found in the differentiation zone but not in the elongation zone. The importance of cell wall-bound trans-and ci.s-ferulic acids is discussed in the context of root growth and differentiation.

<i>Azospirillum</i> Growth Promotion Is Related to Changes in Ferulate and Dehydrodiferulate Contents in Cell Wall of Inoculated Cucumber Seedlings Hypocotyls

“…Cell wall-bound phenolics were extracted according to the method described by [17] with slight modifications. Briefly, 0.2 g DW of each pooled hypocotyl segment tissue was crushed in liquid nitrogen, then extracted with methanol/distilled water (80/20, v/v) and absolute ethanol consecutively, and finally centrifuged at 930 × g for 10 min.…”

“…Precisely how CW components and their intermolecular interactions are changed to allow CW stiffening during growth cessation is not completely known [17]. Azospirillum inoculation induced higher CW elasticity a biophysical indication of CW induced differences during plant cell elongation [3].…”

Cis- and Trans-Cinnamic Acids Have Different Effects on the Catalytic Properties of Arabidopsis Phenylalanine Ammonia Lyases PAL1, PAL2, and PAL4

“…The trans-to-cis conversion of CA is able to occur both in vitro under light (Kahnt 1967) and in dark-grown root tissues (Locher et al 1994). However, information regarding the regulatory effects of cis-CA on PAL enzymatic activity remains scarce.…”

“…However, information regarding the regulatory effects of cis-CA on PAL enzymatic activity remains scarce. Cis-cinnamic acid, like cis-p-coumaric acid, cis-ferulic acid, and ciscaffeic acid (Haskins et al 1964;Ohashi et al 1987;Braun and Tevini 1993;Locher et al 1994;Rasmussen and Rudolph 1997;Wu et al 2001), has been found to be naturally occurring (Yin et al 2003). The biological activities of cis-CA have also been demonstrated in plants (Veldstra 1953;Yang et al 1999).…”

Composition of cellular walls in callus cultures of strawberries with different capacity to morphogenesis

“…Другим возможным объяснением этого является модификация состава и свойств клеточной стенки вследствие присоединения феруловой кислоты к полисахаридным остаткам. В литературе в последнее время появились данные об участии фенольных кислот клеточной стенки в регуляции способности клетки к растяжению и формированию агрегатов [7,8 ]. Наши результаты свидетельствуют о ключевой роли этих соединений в процессах регенерации в каллусных культурах.…”