Cell Volume to Aid Analysis and Technique of Somatic Cell Counts in Milk

Abstract

In conjunction with a Coulter Counter, somatic cells in milk were sized by electronic analysis. Quarter milk from cows with mastitis had a cell volume peak with a modal cell volume of 102 mu3 while milk from healthy quarters had no peak. Bulk milks with a peak had higher cell counts than milks where there was no peak. Dimensions of peak from bulk milks were the same as from quarter milks. Modal cell volume and cell count varied after milks were fixed with varying concentrations and types of fixative. The concentration of fixative recommended by the International Dairy Federation was sufficient, but only marginally so, and it should be increased.

Relationship of somatic cell count and cell volume analysis of goat's milk to intramammary infection with coagulase-negative staphylococci

“…A Coulter Channelyzer C1000 was interfaced to the Coulter Counter producing a distribution of the volumes of the cells counted for each milk sample. Details of the Channelyzer were given by Sheldrake et al (1977). The Channelyzer was adjusted to record all particles with a volume between 5 fi 3 and 180 /i 3 .…”

Analysis of somatic cell volume distribution as an aid to the diagnosis of mastitis

“…In samples from mastitic quarters the majority of cells had a cell volume of 50 to 150 /im 3 , with a mode of 102 fim 3 (Sheldrake et al 1977) which in the Model TA is covered by ch 6 and 7. Because of the weighting of channels, a substantial elevation in ch 7 above ch 6 indicates a peak of cells in ch 6 and 7.…”

“…Cell volume analysis has also been used on quarter and bulk milk using the Coulter C1000 Channelyzer (Hoare et al 1975;Sheldrake et al 1977). In samples from mastitic quarters the majority of cells had a cell volume of 50 to 150 /im 3 , with a mode of 102 fim 3 (Sheldrake et al 1977) which in the Model TA is covered by ch 6 and 7.…”

“…Newbould (1975) claimed that electronic analysis of cell volume distributions using the Coulter Counter Model TA assisted in the interpretation of ESCC, and gave examples using mastitic and non-mastitic quarter milk, bulk milk and colostrum. Hoare et al (1975) and Sheldrake et al (1977) reported that cell volume analysis using the Coulter C1000 Channelyzer aided in detecting mastitis in both quarter and herd milk. Newbould (1978), again using the Model TA, gave data on cell count and one channel of the cell volume histogram of milk samples from quarters of known infection status.…”

“…Hoare et al (1975) and Sheldrake et al (1977) reported that cell volume analysis using the Coulter C1000 Channelyzer aided in detecting mastitis in both quarter and herd milk. Newbould (1978), again using the Model TA, gave data on cell count and one channel of the cell volume histogram of milk samples from quarters of known infection status.…”