Cell Volume Sensing and Regulation in Skeletal Muscle Cells

Rasgado-Flores, Héctor; Theobald, Jillian; Ruíz, Jesús A.; Bitner, John B.; Markowitz, Susan; Zlatnick, Darryl; Yee, Pei Ang; Yee, Pei Ping; Trais, Lauren; Gohar, Kathy; Hergan, David; Buechler, Robert; Lajvardi, Robert; Peña‐Rasgado, C.

doi:10.1007/0-387-23752-6_25

Cited by 2 publications

(2 citation statements)

References 52 publications

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“…It has been observed that muscle dehydration affects muscle contractile capacity [84,95]. The molecular mechanisms by which ICW affects muscle contractile capacity are, however, poorly understood, although some studies have suggested that, on the surface of hydrophilic substances (such as proteins), water is ordered or structured in such a way that increases its density and/or viscosity up to six times.…”

Section: Water’s Role In Mechanical Muscle Functionmentioning

confidence: 99%

The Role of Water Homeostasis in Muscle Function and Frailty: A Review

2019

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Water, the main component of the body, is distributed in the extracellular and intracellular compartments. Water exchange between these compartments is mainly governed by osmotic pressure. Extracellular water osmolarity must remain within very narrow limits to be compatible with life. Older adults lose the thirst sensation and the ability to concentrate urine, and this favours increased extracellular osmolarity (hyperosmotic stress). This situation, in turn, leads to cell dehydration, which has severe consequences for the intracellular protein structure and function and, ultimately, results in cell damage. Moreover, the fact that water determines cell volume may act as a metabolic signal, with cell swelling acting as an anabolic signal and cell shrinkage acting as a catabolic signal. Ageing also leads to a progressive loss in muscle mass and strength. Muscle strength is the main determinant of functional capacity, and, in elderly people, depends more on muscle quality than on muscle quantity (or muscle mass). Intracellular water content in lean mass has been related to muscle strength, functional capacity, and frailty risk, and has been proposed as an indicator of muscle quality and cell hydration. This review aims to assess the role of hyperosmotic stress and cell dehydration on muscle function and frailty.

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Section: Water’s Role In Mechanical Muscle Functionmentioning

confidence: 99%

The Role of Water Homeostasis in Muscle Function and Frailty: A Review

2019

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“…Cell volume is largely determined by the intracellular osmolyte concentration. Thus changes in cell volume under iso-or hypotonic conditions result from net gain or loss of intracellular osmolytes (1)(2)(3)(4).…”

Section: Introductionmentioning

confidence: 99%

Effect of extracellular glucose and K+ on intracellular osmolytes and volume in a human kidney cell line

Hubert

Indyk²,

Peña‐Rasgado³

et al. 2007

Front Biosci

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The goal of this study was to assess the effect of extracellular glucose and K+ ((K)o) on the intracellular osmolyte content and cell volume maintenance and regulation in a human embryonic kidney cell line (tsA201a). Cell volume maintenance was studied by isotonic (313 +/- 5 mOsm) replacement of culture media by a glucose-free Ringer solution containing (in mM) 0, 3, 6, or 10 K+. Cell volume regulation was studied by exposing cells to hypotonic (250 +/- 5 mOsm) glucose-free Ringer solution containing the various (K)o. The results showed that: 1) intracellular osomlyte content (i.e. Na+, Cl-, Urea and free amino acids (FAA)) and cell volume increased when culture media was replaced with isotonic Ringer at all (K)o; 2) osmolyte content decreased with continuous exposure to isotonic Ringer at all (K)o but cell volume changes depended on (K)o. Volume recovery occurred at 6 and 10 mM K+; 3) exposure to hypotonic Ringer induced swelling at all (K)o followed by a reduction in measured intracellular osmolytes. Regulatory volume decrease occurred in 6 or 10 mM K+ but swelling continued in 0 or 3 mM K+; and 4) addition of ouabain produced swelling without recovery under iso- and hypotonic conditions. These results indicate that the removal of extracellular glucose produced a transient inhibition of the Na+/K+ ATPase resulting in a transient increase in the intracellular content of Urea, FAA and cell volume and (K)o regulated an as yet unidentified intracellular osmolyte.

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Cell Volume Sensing and Regulation in Skeletal Muscle Cells

Cited by 2 publications

References 52 publications

The Role of Water Homeostasis in Muscle Function and Frailty: A Review

The Role of Water Homeostasis in Muscle Function and Frailty: A Review

Effect of extracellular glucose and K+ on intracellular osmolytes and volume in a human kidney cell line

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