Cell Viability Inhibition Effect of Gallic Acid and Its Synthetic Derivative Forms on Primary Cultured Endometriosis Cells

Bustami, Arleni; Nareswari, Salma S. K; Sopiah, Popi; Zoya, M. A; Ade, Alex; Muharram, Raden

doi:10.1166/asl.2017.9371

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“…With the ability of octyl gallate and heptyl gallate which can affect the mechanism of proinflammatory regulation by suppressing the NFkB pathway and COX-2 secretion, the inflammatory process could be prevented and or suppressed, so the process of proliferation, invasion, angiogenesis, and persistence of ectopic implants in endometriosis cells was not occurred. This was in line with the results of our previous studies that demonstrated the ability of gallate acid, heptyl gallate, and octyl gallate to reduce cell proliferation and increase apoptotic endometriosis cells [24,25]. Likewise, previous studies reported that the alkyl ester derivatives of gallic acid such as heptyl gallate and octyl gallate had the ability to suppress proliferation and induced apoptosis in some cancer cells [12].…”

Section: Discussionsupporting

confidence: 92%

2441 Interleukin-1b and Cyclooxygenase-2 Proinflammation Analysis and in Silico Docking Nuclear Factor Kappa B on Endometriosis Cell Culture Given Heptyl Gallate and Octyl Gallate Treatment

Bustami

Utami

Budiarti

et al. 2019

Asian J Pharm Clin Res

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Objective: The aim of this study is to analyze the effect of octyl gallate and heptyl gallate toward the regulation of interleukin-1β and cyclooxygenase (COX)-2 proinflammatory factor on endometriosis cell culture and analyze its activity toward nuclear factor kappa B (NFkB) target protein through in silico docking technique. Methods: In vitro study was performed on endometriosis cells cultured treated with two dosages each of heptyl and octyl gallate (51.2 μg/mL and 102.4 μg/mL) for 48 h, then followed by 10 ng/mL lipopolysaccharides (LPS) induction for 24 h. The positive control group was treated by LPS induced and the negative control was treated without LPS. Inflammation regulation was evaluated with enzyme-linked immunosorbent assay technique and in silico docking analyzed using bioinformatics technique. Results: Molecular docking analysis with gallic acid and their derivatives showed that more stable affinity and stronger binding found on octyl gallate than heptyl gallate and gallic acid at the active site of NFkB. Conclusions: Based on this study results, octyl gallate and heptyl gallate were proven to be able to reduce COX-2 proinflammatory factor through NFkB pathway as an inflammatory regulator; thus, it has the potential to be developed as a therapy for endometriosis.

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Section: Discussionsupporting

confidence: 92%