Cell viability in the cadmium-stressed cell suspension cultures of tobacco is regulated by extracellular ATP, possibly by a reactive oxygen species-associated mechanism

Shi, Zhenzhen; Cao, Jiaxin; Pang, Hailong; Lee, Jia; Sun, Kun; Feng, Hongmei

doi:10.32604/biocell.2020.08059

Cited by 2 publications

(2 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Data are presented as the average of three replicates. Asterisks mark statistically significant differences (*P < 0.05) between the control and treatment conditions, determined using a one way ANOVA with the LSD post hoc test uM) was measured through ion conductance analysis (Shi et al 2020). Cd treatment affected the growth of plant cultured cells, increased ROS, and caused a change in antioxidant substances.…”

Section: Effects Of CD Treatment In Suspension-cultured Cellsmentioning

confidence: 99%

Differential responses of peroxidases in sweetpotato suspensioncultured cells to cadmium treatment

et al. 2023

View full text Add to dashboard Cite

As cultured plant cells can grow in high oxidative stress conditions, they form an excellent system to study antioxidant mechanisms and the mass production of antioxidants. Oxidative stress is a major cause of damage in plants exposed to various types of environmental stress, including heavy metals, such as cadmium (Cd). Heavy metal accumulation can interfere with many cell functions and plant growth. To evaluate the contribution of oxidative stress to Cd-induced toxicity, cultured sweetpotato (Ipomoea batatas) cells were treated with increasing concentrations of Cd (0, 10, 25, and 50 μM) and cultured further. Cell growth was significantly inhibited by 25 and 50 μM of Cd, and the total protein content increased with 50 μM of Cd. Additionally, the activity of peroxidase (POD) and ascorbate peroxidase (APX), antioxidant enzymes that remove hydrogen peroxide (a reactive oxygen species), increased in the cells after treatment with 50 μM of Cd. The expression analysis of POD, APX, and peroxiredoxin (PRX) isolated from sweetpotato cultured cells in a previous study revealed the differential expression of POD in response to Cd. In this study, the expression levels of several acidic POD (swpa2, swpa3, and swpa4) and basal POD (swpb1, swpb2, and swpb3) genes were increased in Cd-treated cultured cells. These results indicate that Cd-mediated oxidative stress is closely linked to improved POD-mediated antioxidant defense capacity in sweetpotato suspension-cultured cells.

show abstract

Section: Effects Of CD Treatment In Suspension-cultured Cellsmentioning

confidence: 99%

Differential responses of peroxidases in sweetpotato suspensioncultured cells to cadmium treatment

et al. 2023

View full text Add to dashboard Cite

show abstract

“…The plants exposed to Cd, especially in higher concentrations, show toxicity-related symptoms such as generation of reactive oxygen species (ROS) resulted lipid peroxidation, enzyme inactivation and DNA damage [25,26], reduced photosynthesis [27], inhibited respiration and gas exchange [28,29] and cell proliferation [30], diminished water balance [31], and disturbed carbohydrate [32] and nutrient uptake metabolisms [33] resulting in visible symptoms such as chlorosis, necrosis, root blackening, stunting and general reductions in biomass production [34][35][36]. Due to the above effects, Cd causes a decrease in yield and quality, especially in field crops.…”

Section: Introductionmentioning

confidence: 99%

The effects of cadmium on growth, some anatomical and physiological parameters of wheat (Triticum aestivum L.)

Özyiğit

Baktibekova

Hocaoğlu-Özyiğit

et al. 2021

International Journal of Life Sciences and Biotechnology

View full text Add to dashboard Cite

Nowadays, increased population and traffic density, together with the development of industry, caused increasing levels of heavy metals releasing to the environment, and environmental pollution has reached its highest level worldwide. Chemical products, fertilizers, industrial dyes, construction materials, silver dental fillings and vaccines are some of the well-known sources of heavy metals exposed the environment. Toxic heavy metals can normally be present in body parts of living things at very low levels, but at higher concentrations they can show toxic effects depending on species and duration. Among heavy metals, cadmium is one of the most harmful ones to the environment, humans, animals and plants, and can be toxic even at low concentrations. Thus in this study, Cd was applied to the wheat (Triticum aestivum L.) plants grown in Kyrgyzstan in different concentrations (0, 50, 100, 200 and 400 μM for experimental groups) and in addition to accumulations in different plant parts, some growth, development, physiological and anatomic parameters were measured. As a result, it was observed that wheat plants were affected by all Cd concentrations, although they were able to manage lower stress in terms of some parameters. It was also seen that plants were negatively affected by higher levels of Cd stress, although remained alive throughout the experimental period.

show abstract

Cell viability in the cadmium-stressed cell suspension cultures of tobacco is regulated by extracellular ATP, possibly by a reactive oxygen species-associated mechanism

Cited by 2 publications

References 39 publications

Differential responses of peroxidases in sweetpotato suspensioncultured cells to cadmium treatment

Differential responses of peroxidases in sweetpotato suspensioncultured cells to cadmium treatment

The effects of cadmium on growth, some anatomical and physiological parameters of wheat (Triticum aestivum L.)

Contact Info

Product

Resources

About