Cell Types of the Islets of Langerhans and the Hormones They Produce

Lazarow, Arnold

doi:10.2337/diab.6.3.222

Cited by 28 publications

(5 citation statements)

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“…The existence of a second secretory process, involving intracellular solubilization of the granules has been suggested by L~z~_~ow [23]. Confirmatory evidence of such a process in Aeomys may be inferred from observations made on four siblings of a diabetic mother, two of which were examined at birth,, when glycosuria was present, while the other two were observed on the next day, when glycosuria had disappeared.…”

Section: % I~ictet Et Al : I-iyperplastio Islets Of Langerhans Of Smentioning

confidence: 60%

Elektronenmikroskopische Untersuchungen der hyperplastischen Langerhans'schen Inseln der Stachelmaus (Acomys cahirinus) vor und w�hrend der Entwicklung eines diabetischen Syndroms

et al. 1967

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Section: % I~ictet Et Al : I-iyperplastio Islets Of Langerhans Of Smentioning

confidence: 60%

Elektronenmikroskopische Untersuchungen der hyperplastischen Langerhans'schen Inseln der Stachelmaus (Acomys cahirinus) vor und w�hrend der Entwicklung eines diabetischen Syndroms

et al. 1967

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“…He found that 62 per cent of the insulin was isolated with the mitochondria, but he could not resolve the question of whether or not the insulin was contained in a separate secretion granule. Lazarow (9) also found that the majority of the insulin in the islet of the toadfish (Opsanus tau) could be sedimented with low gravitational forces.…”

Section: Introductionmentioning

confidence: 98%

Isolation of an Insulin Secretion Granule Fraction

Lindall

Bauer

Dixit

et al. 1963

The Journal of Cell Biology

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Goosefish islets were homogenized in 0.25 M sucrose and separated into nuclear, mitochondrial + secretion granule, microsomal, and supernatant fractions. Eighty per cent of the cytochrome oxidase activity and 75 per cent of the bioassayed insulin activity were found in the mitochondrial + secretion granule fraction (6000 g for 10 minutes). The mitochondrial + secretion granule fraction was further subfractionated by centrifugation (2 hours at 100,000 g and 0°C) using a continuous linear density gradient 1.0–2.0 M sucrose). Eighteen to 20 subfractions were collected by piercing the bottom of the tube and collecting drops. The total protein was distributed into a bimodal curve consisting of a high density component, which contained 90 per cent of the insulin (secretion granules), and a lower density component, which contained the cytochrome oxidase activity (mitochondria).

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“…Histologically, different cellu lar components of a teleostean islet appear to be similar to those found in mammals (B o w ie (1924], Lazarow [1957], Mosca [1957,1958], K iianna |1902[). G rosso [1950], F alkm er and O lsson [1962] K iia Ní NA [ 1962] and K iianna and Mehrotra |1968| have described three morphologically distinct cell types in the fishes studied by them .…”

Section: Discussionmentioning

confidence: 98%