Cell types and neuronal circuitry underlying female aggression in Drosophila

Schretter, Catherine E.; Aso, Yoshinori; Robie, Alice A.; Dreher, Marisa; Dolan, Michael J.; Chen, Nan; Ito, Masayoshi; Yang, Tansy; Parekh, Ruchi; Branson, Kristin; Rubin, Gerald M.

doi:10.7554/elife.58942

Cited by 77 publications

(107 citation statements)

References 102 publications

(232 reference statements)

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“…An innate behavioral repertoire is thought to be initiated by higher order brain centers that represent a specific motivational state, or drive 1,[19][20][21][22]52,53 . These centers are activated by stimuli relevant to the drive and then select an appropriate motor program for action 16,23,24,54 .…”

Section: Discussionmentioning

confidence: 99%

Decisions in an Innate Behavioral Sequence

2021

Preprint

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Innate behaviors are comprised of ordered sequences of component actions that progress to satisfy drives. We have characterized the structure of egg-laying behavior in Drosophila in detail and observed that the sequence is not merely comprised of motor acts but also acts of sensory exploration that govern the transitions between component actions. We have identified a cluster of internal sensory neurons that provide information about the progression of the egg during ovipositor burrowing, a behavior necessary for the subterraneous deposition of the egg. These neurons impart sensory feedback that allows burrowing to continue to egg deposition or to abort in favor of further exploration. Diminished activity of these neurons upon completed egg expulsion may initiate the transition to the final phase of egg-laying, allowing the cycle to repeat. Sensory feedback therefore plays a critical role at decision points between transitions affording innate behaviors with an adaptive flexibility.

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Section: Discussionmentioning

confidence: 99%

Decisions in an Innate Behavioral Sequence

2021

Preprint

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“…Bulk quantitative analysis. We evaluated the accuracy of PatchPerPixMatch quantitatively on 10 target neuron morphologies and respective 47 known matching GAL4 lines [17,19,18], as listed in Appendix A, Figure 8. For each known matching GAL4 line, we determined two different ranks: (1) The rank of the best-matching MCFO acquisition of the GAL4 line among all ∼20,000 searched MCFO acquisitions (termed sample rank), and (2) the rank of the best-matching MCFO acquisition of the GAL4 line among all ∼3,500 best-matching MCFO acquisitions per GAL4 line (termed line rank).…”

Section: Resultsmentioning

confidence: 99%

“…Appendix B, Figure 10 lists the first Fig. 4: Exemplary pC1d and pC1e neurons (excerpts from Figure 4 -figure supplements 3D and 4D of [17]). pC1d features a distinctive arbor, see arrow.…”

Section: Resultsmentioning

confidence: 99%

PatchPerPixMatch for Automated 3d Search of Neuronal Morphologies in Light Microscopy

Mais

Hirsch

Managan

et al. 2021

Preprint

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Studies of individual neurons in the Drosophila nervous system are facilitated by transgenic lines that sparsely and repeatably label respective neurons of interest. Sparsity can be enhanced by means of intersectional approaches like the split-GAL4 system, which labels the positive intersection of the expression patterns of two (denser) GAL4 lines. To this end, two GAL4 lines have to be identified as labelling a neuron of interest. Current approaches to tackling this task include visual inspection, as well as automated search in 2d projection images, of single cell multi-color flip-out (MCFO) acquisitions of GAL4 expression patterns. There is to date no automated method available that performs full 3d search in MCFO imagery of GAL4 lines, nor one that leverages automated reconstructions of the labelled neuron morphologies. To close this gap, we propose PatchPerPixMatch, a fully automated approach for finding a given neuron morphology in MCFO acquisitions of Gen1 GAL4 lines. PatchPerPixMatch performs automated instance segmentation of MCFO acquisitions, and subsequently searches for a target neuron morphology by minimizing an objective that aims at covering the target with a set of well-fitting segmentation fragments. PatchPerPixMatch is computationally efficient albeit being full 3d, while also highly robust to inaccuracies in the automated neuron instance segmentation. We are releasing PatchPerPixMatch search results for ~30,000 neuron morphologies from the Drosophila hemibrain in ~20,000 MCFO acquisitions of ~3,500 Gen1 GAL4 lines.

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“…For example, genes annotated as being involved in cholinergic synaptic transmission are overrepresented among socially responsive genes (GO:0007271, P = 0.0015), suggesting that cholinergic neurons may play a role in the aggressive encounters between the wasps. Recent work in Drosophila has implicated cholinergic signaling in aggression in both males and females 54–56 , suggesting potentially shared mechanisms related to aggressive interactions across taxa.…”

Section: Resultsmentioning

confidence: 99%

Dynamic neurogenomic responses to social interactions and dominance outcomes in female paper wasps

Jernigan

Zaba

et al. 2021

Preprint

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Social interactions have large effects on individual physiology and fitness. In the immediate sense, social stimuli are often highly salient and engaging. Over longer time scales, competitive interactions often lead to distinct social ranks and differences in physiology and behavior. Understanding how initial responses lead to longer-term effects of social interactions requires examining the changes in responses over time. Here we examined the effects of social interactions on transcriptomic signatures at two points, at the end of a 45-minute interaction and 4 hours later, in female P. fuscatus paper wasp foundresses. Female P. fuscatus have variable facial patterns that are used for visual individual recognition, so we separately examined the transcriptional dynamics in the optic lobe and the central brain. Results demonstrate much stronger transcriptional responses to social interactions in the central brain compared to the optic lobe. Differentially regulated genes in response to social interactions are enriched for memory-related transcripts. Comparisons between winners and losers of the encounters revealed similar overall transcriptional profiles at the end of an interaction, which significantly diverged over the course of 4 hours, with losers showing changes in expression levels of genes associated with aggression and reproduction in paper wasps. On nests, subordinate foundresses are less aggressive, do more foraging and lay fewer eggs compared to dominant foundresses and we find losers shift expression of many genes, including vitellogenin, related to aggression, worker behavior, and reproduction within hours of losing an encounter. These results highlight the early neurogenomic changes that likely contribute to behavioral and physiological effects of social status changes in a social insect.

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Cell types and neuronal circuitry underlying female aggression in Drosophila

Cited by 77 publications

References 102 publications

Decisions in an Innate Behavioral Sequence

Decisions in an Innate Behavioral Sequence

PatchPerPixMatch for Automated 3d Search of Neuronal Morphologies in Light Microscopy

Dynamic neurogenomic responses to social interactions and dominance outcomes in female paper wasps

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