Cell-type Specificity and Developmental Expression of Neural Cell-surface Components Involved in Cell Interactions and of Structurally Related Molecules

Schachner, Melitta; Faissner, Andréas; Kruse, Jan; Lindner, J.; Meier, Doris; Rathjen, Fritz G.; Wernecke, H

doi:10.1101/sqb.1983.048.01.060

Cited by 63 publications

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“…The cellular mechanism(s) of action of the conditioning factors are unknown although one component of brain-conditioned medium (CM) that promotes growth cone motility is a fibronectin-like molecule [Mattson and Kater, 19881. Fibronectin and laminin are among the extracellular matrix molecules believed to control neuronal outgrowth by interacting with specific cell surface nxeptors Welman, 1983;Manthorpe et al, 1983;Rogers et al, 1983;Rutishauser, 1984;Schachner et al, 1983;Bozyczko and Horwitz, 19861. Taken together, these data indicate that specific interactions of the growth substrate and cell-associated molecules are likely to be important regulators of the filopodial and lamellipodial movements of growth cones.…”

Section: Introductionmentioning

confidence: 99%

Components of neurite outgrowth that determine neuronal cytoarchitecture: Influence of calcium and the growth substrate

Mattson

Guthrie

Kater

1988

J of Neuroscience Research

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The present study examined the cellular mechanisms underlying the generation of neuroarchitecture. Identified Helisoma neurons in isolated cell culture normally require factors present in brain-conditioned medium (CM) in order to display the different components of neurite outgrowth (sprouting, elongation, branching, and growth cone motility), which ultimately determine their overall architecture. We report here that cell calcium and cell-substrate interactions can play quite specific roles in the regulation of these different components of neuronal outgrowth. CM-induced neurite outgrowth was inhibited by calcium ionophore A23187. In the absence of CM the calcium channel blocker La3+ (10 microM) reduced intracellular calcium levels and induced neurite sprouting and elongation; growth cone motility and branching were greatly reduced in the La3+-induced neurites. Neurons plated into an environment containing La3+ and a fibronectin substrate exhibited all of the components of neuronal outgrowth normally seen in response to CM. Fibronectin alone had little outgrowth-promoting activity. Neurite elongation rates and branching were increased by exposure to La3+ in neurons on either a CM or fibronectin substrate. The neurons growing on CM or fibronectin whose outgrowth was accelerated by La3+ elaborated neuritic arbors that differed from those of neurons grown in response to CM; differences were seen in neurite length, area of outgrowth, branching frequency, and varicosity numbers. Taken together, these results indicate that 1) calcium and the growth substrate can exert specific effects on neurite sprouting, elongation, growth cone motility, and branching; 2) appropriate levels of activation of these two systems can elicit neurite outgrowth that closely resembles that induced by endogenous growth factors; 3) both the differential expression of the separate components of outgrowth and the kinetics of outgrowth determine a neuron's morphology.

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Section: Introductionmentioning

confidence: 99%

Components of neurite outgrowth that determine neuronal cytoarchitecture: Influence of calcium and the growth substrate

Mattson

Guthrie

Kater

1988

J of Neuroscience Research

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“…More recently, the application of hybridoma technology to the study of mouse neural cell surfaces has led to the description of another membrane glycoprotein, called LI antigen, which seems to be functionally related to N-CAM in that anti-LI Fab fragments inhibit the calcium-independent aggregation of mouse cerebellar cells and neuroblastoma N2A cells Schachner, 1983, 1984;Schachner et al, 1983). These Fab fragments have also been shown to modify the migration of external granular cells from mouse cerebellum in an in vitro migration system .…”

Section: Introductionmentioning

confidence: 99%

Comparison of two cell surface molecules involved in neural cell adhesion.

Rathjen¹,

Rutishauser²

1984

The EMBO Journal

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Two cell surface molecules found in mouse brain, N‐CAM and the L1 antigen, were compared in terms of their cell adhesion function, polypeptide structures, antigenic determinants and distribution in cerebellar tissue. Fab fragments of polyclonal antibodies to either N‐CAM or L1 antigen only partially inhibited the rate of calcium‐independent aggregation of neuroblastoma N2A cells, whereas complete and more efficient inhibition was obtained when they were used in combination. Despite the functional similarity, comparison of the electrophoretic behaviour of the purified molecules and of their proteolytic fragments shows that the L1 antigen polypeptide is distinct from that of N‐CAM. In addition, no antigenic cross‐reactivity was detected between the two molecules. In cryostat sections of cerebellum from young post‐natal mice, N‐CAM was found to be present in all cell and neurite layers, whereas L1 antigen was expressed only in regions containing post‐mitotic cells. These results indicate that two chemically and histochemically distinct cell surface polypeptides can contribute to the calcium‐independent adhesiveness of neural cells, and suggest that their differential expression might cause adhesive specificity among cells of developing neural tissues.

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“…Although a sufficient number of cells was a prerequisite factor for cell differentiation in vitro (Deuchar 1970), the high RBE values for tritium in cell differentiation in the MBC system (Wang et al 1996) might not be ascribed only to a lowered cell density due to the significant accumulation of p53 protein, and the prolonged time of G, and growth arrest. Disturbance of cell aggregation, damage of cellular surface structures, interference of the intracellular interaction of the surviving cells and of their cell cycle, and activation of certain genes should also be taken into account (Schachner et al 1983;Moscana 1975;Canman and Kastan 1997;Walker et al 1993). On the other hand, as there are many factors affecting the RBE values, such as the type of tritium compounds, experimental materials and systems, the endpoints, dose estimation, and reference radiations (Straume and Carsten 1993), multiple mechanisms may be involved.…”

Section: Discussionmentioning

confidence: 98%

Induction Of Apoptosis By Beta Radiation From Tritium Compounds In Mouse Embryonic Brain Cells

Wang¹,

Takeda²,

Gao³

et al. 1999

Health Physics

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Induction of apoptosis by tritium exposure was investigated in both cultured embryonic mid brain cells and brain sections of embryos and of newborns in mice. In the cultures of mid brain cells, addition of methyl-3H-thymidine (3H-TdR) (21 kBq mL(-1)) and tritiated water (5.616 MBq mL(-1)) induced late appearances and low percentages of apoptosis when compared to x-irradiation at the ID50 dose, the inhibitory dose that reduced cellular differentiation by 50% of the control. A significant increase in p53 protein was detected about 2 h before the marked appearance of apoptosis. The pregnant mice were given an intraperitoneal injection of tritiated water at the concentration of 481.8 kBq g(-1) of body weight on gestation day 12.5, by which treatment behavioral changes in the offspring occurred. Increased apoptotic cells were observed in the neural tube of embryos from 1 d after the injection to 1 wk postnatal age. Apoptosis induced by x-rays appeared 2 h after irradiation, with a peak at 4 h. Increase of apoptotic cells was also found in the brain cortexes of newborns. The percentage of apoptosis in the brain was higher in the prenatal tritiated water exposed mice than in the prenatal x-irradiated mice. Possible mechanisms on apoptosis and its relation to the higher relative biological effectiveness value of tritium beta-rays are discussed.

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Cell-type Specificity and Developmental Expression of Neural Cell-surface Components Involved in Cell Interactions and of Structurally Related Molecules

Cited by 63 publications

References 0 publications

Components of neurite outgrowth that determine neuronal cytoarchitecture: Influence of calcium and the growth substrate

Components of neurite outgrowth that determine neuronal cytoarchitecture: Influence of calcium and the growth substrate

Comparison of two cell surface molecules involved in neural cell adhesion.

Induction Of Apoptosis By Beta Radiation From Tritium Compounds In Mouse Embryonic Brain Cells

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