Cell-type-specific limitation onin vivo serotonin storage following ectopic expression of theDrosophila serotonin transporter, dSERT

George, Rebecca; Cai, Ying; Chang, Hui-Yun; Krantz, David E.; Friggi-Grelin, Florence; Birman, Serge; Hirsh, Jay

doi:10.1002/neu.20222

Cited by 16 publications

(12 citation statements)

References 60 publications

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“…This indicates a specific serotonin uptake transporter. Only serotonergic neurons express SERT, and even if SERT is ectopically expressed in other neurons, only very few cells that em- ploy other biogenic amines as transmitters are capable of specifically taking up serotonin (Park et al, 2006). Moreover, the number of SERT-neurons in our stainings of the frontal ganglion did not at any time exceed the maximum number of serotonergic neurons found at later stages.…”

Section: Correspondence Of Sert-expressing and Serotonin-synthesizingmentioning

confidence: 42%

Embryonic differentiation of serotonin‐containing neurons in the enteric nervous system of the locust (Locusta migratoria)

Stern¹,

Knipp²,

Bicker³

2007

J of Comparative Neurology

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The enteric nervous system (ENS) of the locust consists of four ganglia (frontal and hypocerebral ganglion, and the paired ingluvial ganglia) located on the foregut, and nerve plexus innervating fore- and midgut. One of the major neurotransmitters of the ENS, serotonin, is known to play a vital role in gut motility and feeding. We followed the anatomy of the serotonergic system throughout embryonic development. Serotonergic neurons are generated in the anterior neurogenic zones of the foregut and migrate rostrally along the developing recurrent nerve to contribute to the frontal ganglion. They grow descending neurites, which arborize in all enteric ganglia and both nerve plexus. On the midgut, the neurites closely follow the leading migrating midgut neurons. The onset of serotonin synthesis occurs around halfway through development-the time of the beginning of midgut closure. Cells developing to serotonergic phenotype express the serotonin uptake transporter (SERT) significantly earlier, beginning at 40% of development. The neurons begin SERT expression during migration along the recurrent nerve, indicating that they are committed to a serotonergic phenotype before reaching their final destination. After completion of the layout of the enteric ganglia (at 60%) a maturational phase follows, during which serotonin-immunoreactive cell bodies increase in size and the fine arborizations in the nerve plexus develop varicosities, putative sites of serotonin release (at 80%). This study provides the initial step for future investigation of potential morphoregulatory functions of serotonin during ENS development.

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Section: Correspondence Of Sert-expressing and Serotonin-synthesizingmentioning

confidence: 42%

Embryonic differentiation of serotonin‐containing neurons in the enteric nervous system of the locust (Locusta migratoria)

Stern¹,

Knipp²,

Bicker³

2007

J of Comparative Neurology

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“…First, the dSert is highly conserved between mammals and Drosophila and takes up serotonin into the presynaptic neuron after release [17, 18]. Second, dSert is exclusively expressed in serotonergic neurons [19], and third, the transformation of non-serotonergic neurons into serotonergic neurons by overexpression of an UAS-dSert transgene is highly unlikely because this transformation depends on excess serotonin levels not present in animals [20]. Finally this tool allows fine-tuning of serotonin signaling by blocking serotonin reuptake, thereby mimicking the effect of serotonin reuptake inhibitors.…”

Section: Introductionmentioning

confidence: 99%

A Single Pair of Serotonergic Neurons Counteracts Serotonergic Inhibition of Ethanol Attraction in Drosophila

Xu¹,

He²,

Kaiser³

et al. 2016

PLoS ONE

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Attraction to ethanol is common in both flies and humans, but the neuromodulatory mechanisms underlying this innate attraction are not well understood. Here, we dissect the function of the key regulator of serotonin signaling—the serotonin transporter–in innate olfactory attraction to ethanol in Drosophila melanogaster. We generated a mutated version of the serotonin transporter that prolongs serotonin signaling in the synaptic cleft and is targeted via the Gal4 system to different sets of serotonergic neurons. We identified four serotonergic neurons that inhibit the olfactory attraction to ethanol and two additional neurons that counteract this inhibition by strengthening olfactory information. Our results reveal that compensation can occur on the circuit level and that serotonin has a bidirectional function in modulating the innate attraction to ethanol. Given the evolutionarily conserved nature of the serotonin transporter and serotonin, the bidirectional serotonergic mechanisms delineate a basic principle for how random behavior is switched into targeted approach behavior.

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“…One complication of the latter approach is that serotonergic and dopaminergic systems may assume each other's function under certain experimental or natural conditions. In Drosophila , for example, ectopic expression of the serotonin transporter molecule (dSERT) leads to the ability to store 5HT in serotonergic and in dopaminergic neurons in the nerve cord [54]. This suggests that the machinery for transmitter storage is similar in 5HT- and DA-containing neurons, and different from that found in other types of neurons where uptake of amines is not observed.…”

Section: Discussionmentioning

confidence: 99%

Targeted Manipulation of Serotonergic Neurotransmission Affects the Escalation of Aggression in Adult Male Drosophila melanogaster

2010

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Dopamine (DA) and serotonin (5HT) are reported to serve important roles in aggression in a wide variety of animals. Previous investigations of 5HT function in adult Drosophila behavior have relied on pharmacological manipulations, or on combinations of genetic tools that simultaneously target both DA and 5HT neurons. Here, we generated a transgenic line that allows selective, direct manipulation of serotonergic neurons and asked whether DA and 5HT have separable effects on aggression. Quantitative morphological examination demonstrated that our newly generated tryptophan hydroxylase (TRH)-Gal4 driver line was highly selective for 5HT-containing neurons. This line was used in conjunction with already available Gal4 driver lines that target DA or both DA and 5HT neurons to acutely alter the function of aminergic systems. First, we showed that acute impairment of DA and 5HT neurotransmission using expression of a temperature sensitive form of dynamin completely abolished mid- and high-level aggression. These flies did not escalate fights beyond brief low-intensity interactions and therefore did not yield dominance relationships. We showed next that manipulation of either 5HT or DA neurotransmission failed to duplicate this phenotype. Selective disruption of 5HT neurotransmission yielded flies that fought, but with reduced ability to escalate fights, leading to fewer dominance relationships. Acute activation of 5HT neurons using temperature sensitive dTrpA1 channel expression, in contrast, resulted in flies that escalated fights faster and that fought at higher intensities. Finally, acute disruption of DA neurotransmission produced hyperactive flies that moved faster than controls, and rarely engaged in any social interactions. By separately manipulating 5HT- and DA- neuron systems, we collected evidence demonstrating a direct role for 5HT in the escalation of aggression in Drosophila.

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Cell-type-specific limitation onin vivo serotonin storage following ectopic expression of theDrosophila serotonin transporter, dSERT

Cited by 16 publications

References 60 publications

Embryonic differentiation of serotonin‐containing neurons in the enteric nervous system of the locust (Locusta migratoria)

Embryonic differentiation of serotonin‐containing neurons in the enteric nervous system of the locust (Locusta migratoria)

A Single Pair of Serotonergic Neurons Counteracts Serotonergic Inhibition of Ethanol Attraction in Drosophila

Targeted Manipulation of Serotonergic Neurotransmission Affects the Escalation of Aggression in Adult Male Drosophila melanogaster

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