Cell type-specific expression of FoxP2 in the ferret and mouse retina

Sato, Chihiro; Iwai-Takekoshi, Lena; Ichikawa, Yoshie; Kawasaki, Hiroshi

doi:10.1016/j.neures.2016.11.008

Cited by 14 publications

(9 citation statements)

References 54 publications

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“…In support with this hypothesis, we found some dRGCs in Gsk3α f/+ β f/f ;α-Cre and littermate control retina positive for the transcription factor Tbr2, described as essential for RGC specification participating in non-image-forming visual circuits ( Figure 7D) (Sweeney et al, 2014(Sweeney et al, , 2019. A small subset of dRGCs also expressed Foxp2, a transcription factor involved in DS-RGC differentiation in mice ( Figure 7D) (Rousso et al, 2016;Sato et al, 2017). These two factors were expressed in a mutually exclusive way in Rpbms-positive dRGCs, suggesting that dRGCs in Gsk3α f/+ β f/f ;α-Cre might encompass several subtypes.…”

Section: Loss Of Either Gsk3a or Gsk3β In Rpcs Leads To Increased Numsupporting

confidence: 62%

Glycogen Synthase Kinase 3 regulates the genesis of the rare displaced ganglion cell retinal subtype

Kisseleff

Vigouroux

Hottin

et al. 2021

Preprint

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Glycogen Synthase Kinase 3 (GSK) proteins (GSK3α and GSK3β) are key mediators of signaling pathways, with crucial roles in coordinating fundamental biological processes during neural development. Here we show that the complete loss of GSK3 signaling in mouse retinal progenitors leads to microphthalmia with broad morphological defects. Both proliferation of retinal progenitors and neuronal differentiation are impaired and result in enhanced cell death. A single wild-type allele of either Gsk3α or Gsk3β is able to rescue these phenotypes. In this genetic context, all cell types are present with a functional retina. However, we unexpectedly detect a large number of cells in the inner nuclear layer expressing retinal ganglion cell (RGC)-specific markers (called displaced RGCs, dRGCs) when at least one allele of Gsk3α is expressed. Excess dRGCs lead to increased number of axons projecting into the ipsilateral medial terminal nucleus, an area of the brain belonging to the non-image-forming visual circuit and poorly targeted by RGCs in wild-type retina. Transcriptome analysis and optomotor response assay suggest that at least a subset of dRGCs in Gsk3 mutant mice are direction-selective RGCs. Our study thus uncovers a unique role of GSK3 in controlling the genesis of dRGCs, a rare and poorly characterized retinal cell type.

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Section: Loss Of Either Gsk3a or Gsk3β In Rpcs Leads To Increased Numsupporting

confidence: 62%

Glycogen Synthase Kinase 3 regulates the genesis of the rare displaced ganglion cell retinal subtype

Kisseleff

Vigouroux

Hottin

et al. 2021

Preprint

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“…The two identified transcription factors, Foxp2 and Tshz2 [ 53 , 54 ] are expressed and GCL-enriched early in postnatal development (P0-P7), however at P14 – P22 Tshz2 expression is reduced but limited to the GCL, while Foxp2 expression extends to include both INL and GCL. This is somewhat surprising since recent reports suggest that retinal Foxp2 expression is restricted to a subset of RGC types [ 55 , 56 ]. However, a transiently broader pattern of expression is suggested by using a Foxp2 Cre crossed into a general reporter [ 55 ].…”

Section: Discussionmentioning

confidence: 96%

Postnatal developmental dynamics of cell type specification genes in Brn3a/Pou4f1 Retinal Ganglion Cells

Muzyka¹,

Brooks

Badea³

2018

Neural Dev

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BackgroundAbout 20–30 distinct Retinal Ganglion Cell (RGC) types transmit visual information from the retina to the brain. The developmental mechanisms by which RGCs are specified are still largely unknown. Brn3a is a member of the Brn3/Pou4f transcription factor family, which contains key regulators of RGC postmitotic specification. In particular, Brn3a ablation results in the loss of RGCs with small, thick and dense dendritic arbors (‘midget-like’ RGCs), and morphological changes in other RGC subpopulations. To identify downstream molecular mechanisms underlying Brn3a effects on RGC numbers and morphology, our group recently performed a RNA deep sequencing screen for Brn3a transcriptional targets in mouse RGCs and identified 180 candidate transcripts.MethodsWe now focus on a subset of 28 candidate genes encoding potential cell type determinant proteins. We validate and further define their retinal expression profile at five postnatal developmental time points between birth and adult stage, using in situ hybridization (ISH), RT-PCR and fluorescent immunodetection (IIF).ResultsWe find that a majority of candidate genes are enriched in the ganglion cell layer during early stages of postnatal development, but dynamically change their expression profile. We also document transcript-specific expression differences for two example candidates, using RT-PCR and ISH. Brn3a dependency could be confirmed by ISH and IIF only for a fraction of our candidates.ConclusionsAmongst our candidate Brn3a target genes, a majority demonstrated ganglion cell layer specificity, however only around two thirds showed Brn3a dependency. Some were previously implicated in RGC type specification, while others have known physiological functions in RGCs. Only three genes were found to be consistently regulated by Brn3a throughout postnatal retina development – Mapk10, Tusc5 and Cdh4.Electronic supplementary materialThe online version of this article (10.1186/s13064-018-0110-0) contains supplementary material, which is available to authorized users.

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“…Finally, comparisons with other species will provide information about the evolution and function of this particular heterotypically-connected RGC circuit. FOXP2 is also found in a subset of RGCs in the ferret 46 and macaque retinas 15 , so it is possible that the F-mini RGC circuit has a homolog in humans.…”

Section: Discussionmentioning

confidence: 99%

An offset ON–OFF receptive field is created by gap junctions between distinct types of retinal ganglion cells

Cooler

Schwartz

2020

Nat Neurosci

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Receptive fields (RFs) are a foundational concept in sensory neuroscience. The RF of a sensory neuron is shaped by the properties of its synaptic inputs from connected neurons. In the early visual system, retinotopic maps define a strict relationship between the location of a cell's dendrites and its RF location in visual space 1-3 . Retinal ganglion cells (RGCs), the output cells of the retina, form dendritic mosaics that tile retinal space and have corresponding RF mosaics that tile visual space 1,2 . The precise location of dendrites in some RGCs has been shown to predict their RF shape 4 . Previously described ON-OFF RGCs have aligned dendrites in ON and OFF synaptic layers, so the cells respond to increments and decrements of light at the same locations in visual space [5][6][7][8] . Here we report a systematic offset between the ON and OFF RFs of an RGC type. Surprisingly, this property does not come from offset ON and OFF dendrites but instead arises from electrical synapses with RGCs of a different type. This circuit represents a new channel for direct communication between ON and OFF RGCs. Using a multi-cell model, we find that offset ON-OFF RFs could improve the precision with which edge location is represented in an RGC population.1

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Cell type-specific expression of FoxP2 in the ferret and mouse retina

Cited by 14 publications

References 54 publications

Glycogen Synthase Kinase 3 regulates the genesis of the rare displaced ganglion cell retinal subtype

Glycogen Synthase Kinase 3 regulates the genesis of the rare displaced ganglion cell retinal subtype

Postnatal developmental dynamics of cell type specification genes in Brn3a/Pou4f1 Retinal Ganglion Cells

An offset ON–OFF receptive field is created by gap junctions between distinct types of retinal ganglion cells

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