Cell type specific adhesion to surfaces functionalised by amine plasma polymers

Abstract

Our previously-obtained impressive results of highly increased C2C12 mouse myoblast adhesion to amine plasma polymers (PPs) motivated current detailed studies of cell resistance to trypsinization, cell proliferation, motility, and the rate of attachment carried out for fibroblasts (LF), keratinocytes (HaCaT), rat vascular smooth muscle cells (VSMC), and endothelial cells (HUVEC, HSVEC, and CPAE) on three different amine PPs. We demonstrated the striking difference in the resistance to trypsin treatment between endothelial and non-endothelial cells. The increased resistance observed for the non-endothelial cell types was accompanied by an increased rate of cellular attachment, even though spontaneous migration was comparable to the control, i.e., to the standard cultivation surface. As demonstrated on LF fibroblasts, the resistance to trypsin was similar in serum-supplemented and serum-free media, i.e., medium without cell adhesion-mediating proteins. The increased cell adhesion was also confirmed for LF cells by an independent technique, single-cell force spectroscopy. This method, as well as the cell attachment rate, proved the difference among the plasma polymers with different amounts of amine groups, but other investigated techniques could not reveal the differences in the cell behaviour on different amine PPs. Based on all the results, the increased resistance to trypsinization of C2C12, LF, HaCaT, and VSMC cells on amine PPs can be explained most probably by a non-specific cell adhesion such as electrostatic interaction between the cells and amine groups on the material surface, rather than by the receptor-mediated adhesion through serum-derived proteins adsorbed on the PPs.

Engineering 3D Cortical Spheroids for an In Vitro Ischemic Stroke Model

“…61 This enhanced cell adhesion on the amine-functionalized surface was thought to be celltype-specific and contributed by the nonspecific binding between cells and the amine group. 62,63 Based on these observations, the nitrogen-containing functional groups in the ULA surface might lead to the observed cell−ULA interaction by presenting amine groups to the dissociated cortical cells. Nevertheless, while the 96-well plate with a ULA surface was shown to exhibit increased protein adsorption under a singleprotein solution of high concentration, protein absorbed on the ULA surface from the cell suspension could also contribute to the partial binding of cortical cells.…”

Bone Marrow Mesenchymal Stromal Cells on Silk Fibroin Scaffolds to Attenuate Polymicrobial Sepsis Induced by Cecal Ligation and Puncture

“…A material surface containing an amine group (–NH 2 ) allows cells, including MSCs, to enhance their attachment [ 37 , 38 ], which affects MSC function and activity [ 39 ]. Cellular adhesion associated with chemical groups, such as amine (–NH 2 ) or carboxyl (–COOH), is attributed to electrostatic interactions between the cells and chemical groups on the material surface, rather than by specific receptor-mediated cell adhesion, including extracellular matrix or RGD peptide [ 40 ]. Nevertheless, an appropriate adhesion strength is necessary to initiate cell proliferation and cellular function.…”

Bioconjugation Strategy for Ceramic Membranes Decorated with Candida Antarctica Lipase B—Impact of Immobilization Process on Material Features

“…Among the modified ceramic membranes, slightly higher values of W adh were found after enzyme immobilization, guaranteeing the strongest interactions between the enzyme-rich surface and water molecules. Finally, the most reduced adhesion properties of the modified ceramics were seen for the materials after application in the enzymatic process [49,50].…”