Cell type-specific activation of actin genes in the early amphibian embryo

Mohun, Tim; Brennan, S.; Dathan, Nina A.; Fairman, S.; Gurdon, J. B.

doi:10.1038/311716a0

Cited by 345 publications

(156 citation statements)

References 27 publications

Supporting

Mentioning

149

Contrasting

Order By: Relevance

“…Although somitogenesis starts at stage 17 (Keller, 2000), the differentiation of the first muscle fibers begins medially (Fig. 1B) at the end of gastrulation (stage 12.5-13) as previously suggested by the first report on sarcomeric actin expression (Mohun et al, 1984). In addition, although Mrf4 and Myogenin have not been reported to be expressed at these stages (Jennings, 1992;Nicolas et al, 1998), we could detect them at a very low level in paraxial mesoderm (Fig.…”

Section: Differentiation Of the Median Myogenic Cells Is Initiated Bementioning

confidence: 45%

See 1 more Smart Citation

Myogenic waves and myogenic programs during Xenopus embryonic myogenesis

Gaspera

Armand

Sequeira

et al. 2012

Developmental Dynamics

View full text Add to dashboard Cite

Background: Although Xenopus is a key model organism in developmental biology, little is known about the myotome formation in this species. Here, we assessed the expression of myogenic regulatory factors of the Myod family (MRFs) during embryonic development and revealed distinct MRF programs. Results: The expression pattern of each MRF during embryonic development highlights three successive myogenic waves. We showed that a first median and lateral myogenesis initiates before dermomyotome formation: the median cell population expresses Myf5, Myod, and Mrf4, whereas the lateral one expresses Myod, moderate levels of Myogenin and Mrf4. The second wave of myoblasts arising from the dermomyotome is characterized by the full MRF program expression, with high levels of Myogenin. The third wave is revealed by Myf5 expression in the myotome and could contribute to the formation of plurinucleated fibers at larval stages. Furthermore, Myf5-or Myod-expressing anlagen are identified in craniofacial myogenesis. Conclusions: The first median and lateral myogenesis and their associated MRF programs have probably disappeared in mammals. However, some aspects of Xenopus myogenesis have been conserved such as the development of somitic muscles by successive myogenic waves and the existence of Myf5-dependent andindependent lineages. Developmental Dynamics 241:995-1007,

show abstract

Section: Differentiation Of the Median Myogenic Cells Is Initiated Bementioning

confidence: 45%

“…In the head, Des appears first, followed by Actc, Ckm, and later MyhE3 and MyhE19 (Fig. 8C), while during myotome formation, Actc is the first expressed, from stage 12.5 in a large paraxial domain (Mohun et al, 1984), followed by Des, MyhE3 and Ckm (Fig. 1B).…”

Section: Identification Of Additional Mrf Programs During Craniofaciamentioning

confidence: 99%

Myogenic waves and myogenic programs during Xenopus embryonic myogenesis

Gaspera

Armand

Sequeira

et al. 2012

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

“…1B Lower Left). This effect was not secondary to a block of dorsal mesodermal differentiation because expression of the notochord marker collagen type II (19) was not affected in these explants; activin-mediated induction of the somite marker muscle actin (20) was modestly reduced or unaffected by Xctr1 misexpression (Fig. 1C, lanes 2 and 4, and data not shown).…”

Section: Resultsmentioning

confidence: 90%

Vertebrate Ctr1 coordinates morphogenesis and progenitor cell fate and regulates embryonic stem cell differentiation

Haremaki

Fraser

Kuo

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Embryogenesis involves two distinct processes. On the one hand, cells must specialize, acquiring fates appropriate to their positions (differentiation); on the other hand, they must physically construct the embryo through coordinated mechanical activity (morphogenesis). In early vertebrate development, fibroblast growth factor (FGF) regulates multiple embryonic events, including germ layer differentiation and morphogenesis; the cellular components that direct FGF signaling to evoke these different responses remain largely unknown. We show here that the copper transporter 1 (Ctr1)

show abstract

“…Then RT-PCR was performed by using a pair of primers for XSM22␣ and for Xenopus cardiac actin (primer 5, 5Ј-TCC CTG TAC GCT TCT GGT CGT A-3Ј; primer 6, 5Ј-TCT CAA AGT CCA AAG CCA CAT A-3Ј) that were expected to amplify the 250-bp fragment as a marker for the skeletal and cardiac muscle lineages. 39,40 These PCR products were subjected to electrophoresis and quantified by NIH image.…”

Section: Injection Of Dominant-negative Mutant Of Fgf Receptormentioning

confidence: 99%

Fibroblast Growth Factor Plays a Critical Role in SM22α Expression During Xenopus Embryogenesis

Oka

Shiojima

Monzen

et al. 2000

ATVB

View full text Add to dashboard Cite

Abstract-Although smooth muscle cells (SMCs) are critical components of the circulatory system, the regulatory mechanisms of SMC differentiation remain largely unknown. In the present study, we examined the mechanism of SMC differentiation by using Xenopus laevis SM22␣ (XSM22␣) as an SMC-specific marker. XSM22␣ cDNA contained a 600-bp open reading frame, and the predicted amino acid sequences were highly conserved in evolution. XSM22␣ transcripts were first detected in heart anlage, head mesenchyme, and the dorsal side of the lateral plate mesoderm at the tail-bud stage, possibly representing the precursors of muscle lineage. At the tadpole stage, XSM22␣ transcripts were restricted to the vascular and visceral SMCs. XSM22␣ was strongly induced by basic fibroblast growth factor (FGF) in animal caps. Although expressions of Xenopus cardiac actin were not affected by the expression of a dominantnegative FGF receptor, its injection dramatically suppressed the XSM22␣ expression. These results suggest that XSM22␣ is a useful molecular marker for the SMC lineage in Xenopus and that FGF signaling plays an important role in the induction of XSM22␣ and in the differentiation of SMCs.

show abstract

Cell type-specific activation of actin genes in the early amphibian embryo

Cited by 345 publications

References 27 publications

Myogenic waves and myogenic programs during Xenopus embryonic myogenesis

Myogenic waves and myogenic programs during Xenopus embryonic myogenesis

Vertebrate Ctr1 coordinates morphogenesis and progenitor cell fate and regulates embryonic stem cell differentiation

Fibroblast Growth Factor Plays a Critical Role in SM22α Expression During Xenopus Embryogenesis

Contact Info

Product

Resources

About