Cell type-dependent alterations of binding of synthetic blood group antigen-related oligosaccharides in lung cancer

Kayser, Klaus; Bovin, Nicolai V.; Zemlyanukhina, T. V.; Donaldo-Jacinto, Sonia; Koopmann, Jens-Oliver; Gabius, Hans‐Joachim

doi:10.1007/bf00731207

Cited by 15 publications

(8 citation statements)

References 51 publications

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“…A number of OS, such as LacNAc, T ββ , GalNAcβ1-4GlcNAc, asialoGM1 and blood group A are known to be potent ligands for cellular lectins such as galectins [26]. Indeed, MDCK exhibited significant level of binding to LacNAc, asialoGM1, GalNAcβ1-4GlcNAc, TF, blood group A (A tri , A tetra ) and Fucα1-2Galβ1-3GlcNAc (Le d ) oligosaccharides as shown in Figure 1.…”

Section: Carbohydrate-binding Pattern Of Mdck and Vero Cellsmentioning

confidence: 91%

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et al. 2006

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Sialyl oligosaccharides have long been considered to be the sole receptors for influenza virus. However, according to [1] some viruses are able to grow in sialic-free MDCK cells. Here we attempted to reveal a possible second, non-sialic receptor, hypothesizing the involvement of additional carbohydrate lectin recognition in influenza virus reception process, first of all in situations when a lectin of the host cell could recognize the viral carbohydrate ligand. We tested the presence of galactose- and sialic acid-binding lectins, as well as mannoside- and sulfo-N-acetyllactosamine-recognizing properties of MDCK and Vero cells using polyacrylamide neoglycoconjugates and antibodies. MDCK cells bind galactoside probes stronger than Vero cells, whereas Vero cells bind preferentially sialoside, mannoside and various sulfo-oligosaccharide probes. The probing of viruses with the neoglycoconjugates revealed specific 6'-HSO (3) LacNAc (but not other sulfated oligosaccharides) binding property of A and B human strains. Affinity of 6'-HSO (3) LacNAc probe was comparable with affinity of 6'-SiaLac probe but the binding was not inhibited by the sialooligosaccharide.

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Section: Carbohydrate-binding Pattern Of Mdck and Vero Cellsmentioning

confidence: 91%

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et al. 2006

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“…Statistical analysis was performed with chi-square test and the t-test. The biotinylated fucan was synthesized and ligandohistochemistry including the adequate specificity controls was performed, as described previously (Zeng et al, 1992;Kayser et al, 1994a). Histochemical reaction was classified to be positive, if all or clusters of pulmonary epithelial cells displayed a dark brown colour, and that of concomitant negative controls no detectable staining intensity.…”

Section: Methodsmentioning

confidence: 99%

Application of Computer‐assisted Morphometry to the Analysis of Prenatal Development of Human Lung

Kayser¹,

Jacinto²,

Böhm³

et al. 1997

Anat Histol Embryol

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Morphometry is well-established in tumour pathology. To evaluate is potential usefulness for description of developmental processes, histological slides from paraffin-embedded specimens of 67 human fetal lungs were Feulgen-stained, and morphometric characteristics of nuclei of epithelial pulmonary cells were analysed with an automated image analysis system. The measured cytometric features comprised of integrated optical density (IOD), S-phase-related IOD fraction, IOD entropy and nuclear area. Histometric features of the specimens were based upon the minimum spanning tree (MST) and included distances between neighboring epithelial cells, between epithelial cells and neighboring lymphocytes, and assessment of MST entropy. Notably, certain parameters revealed a non-uniform level during prenatal development S-phase-related IOD fraction increased from 5% to 8% between 14 and 16 weeks of gestation, then declined to 6% until birth. The IOD entropy steadily increased during development, whereas the extent of nuclear area remained constant. In accordance with an increase of the S-phase-related fraction the MST entropy displayed a singular peak between 14 and 16 weeks of gestation, which is probably associated with development of glandular structures in the lung. Correlation of expression of binding sites for markers, presumably involved in functional aspects of development, with such alterations, is shown for binding capacities of biotinylated fucoidan and the S-phase-related fraction. This may be helpful to infer immuno- or ligando histochemically defined tissue sites with potential physiological significance in morphometrically distinguished periods of development.

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“…Experimental details have been given elsewhere. 26,39 Additionally, gel filtration on Biogel P-10 was performed to remove any aggregates. The lectin was judged to be homogeneous by SDS-PAGE analysis.…”

Section: Carbohydrate-binding Proteinsmentioning

confidence: 99%

Role of aromatic amino acids in carbohydrate binding of plant lectins: Laser photo chemically induced dynamic nuclear polarization study of hevein domain-containing lectins

et al. 1997

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Carbohydrate recognition by lectins often involves the side chains of tyrosine, tryptophan, and histidine residues. These moieties are able to produce chemically induced dynamic nuclear polarization (CIDNP) signals after laser irradiation in the presence of a suitable radical pair-generating dye. Elicitation of such a response in proteins implies accessibility of the respective groups to the light-absorbing dye. In principle, this technique is suitable to monitor surface properties of a receptor and the effect of ligand binding if CIDNP-reactive amino acids are affected. The application of this method in glycosciences can provide insights into the protein-carbohydrate interaction process, as illustrated in this initial study. It focuses on a series of N-acetylglucosamine-binding plant lectins of increasing structural complexity (hevein, pseudohevein, Urtica dioica agglutinin and wheat germ agglutinin and its domain B), for which structural NMR- or X-ray crystallographic data permit a decision of the validity of the CIDNP method-derived conclusions. On the other hand, the CIDNP data presented in this study can be used for a rating of our molecular models of hevein, pseudohevein, and domain B obtained by various modeling techniques. Experimentally, the shape and intensity of CIDNP signals are determined in the absence and in the presence of specific glycoligands. When the carbohydrate ligand is bound, CIDNP signals of side chain protons of tyrosine, tryptophan, or histidine residues are altered, for example, they are broadened and of reduced intensity or disappear completely. In the case of UDA, the appearance of a new tryptophan signal upon ligand binding was interpreted as an indication for a conformational change of the corresponding indole ring. Therefore, CIDNP represents a suitable tool to study protein-carbohydrate interactions in solution, complementing methods such as X-ray crystallography, high-resolution multidimensional nuclear magnetic resonance, transferred nuclear Overhauser effect experiments, and molecular modeling.

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Cell type-dependent alterations of binding of synthetic blood group antigen-related oligosaccharides in lung cancer

Cited by 15 publications

References 51 publications

Search for additional influenza virus to cell interactions

Search for additional influenza virus to cell interactions

Application of Computer‐assisted Morphometry to the Analysis of Prenatal Development of Human Lung

Role of aromatic amino acids in carbohydrate binding of plant lectins: Laser photo chemically induced dynamic nuclear polarization study of hevein domain-containing lectins

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