Cell trapping in microfluidic chips

Johann, Robert

doi:10.1007/s00216-006-0369-6

Cited by 93 publications

(83 citation statements)

References 17 publications

(18 reference statements)

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“…Different methods for single cell trapping have been applied so far, including contactless and contact based methods. 22,23 However, most methods were applied to eukaryotic cells. 24 Evidently, the typically 10-100 times smaller prokaryotic cells used in industrial biotechnology necessitate more precise microfabrication and well device control.…”

Section: Introductionmentioning

confidence: 99%

A disposable picolitre bioreactor for cultivation and investigation of industrially relevant bacteria on the single cell level

et al. 2012

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In the continuously growing field of industrial biotechnology the scale-up from lab to industrial scale is still a major hurdle to develop competitive bioprocesses. During scale-up the productivity of single cells might be affected by bioreactor inhomogeneity and population heterogeneity. Currently, these complex interactions are difficult to investigate. In this report, design, fabrication and operation of a disposable picolitre cultivation system is described, in which environmental conditions can be well controlled on a short time scale and bacterial microcolony growth experiments can be observed by time-lapse microscopy. Three exemplary investigations will be discussed emphasizing the applicability and versatility of the device. Growth and analysis of industrially relevant bacteria with single cell resolution (in particular Escherichia coli and Corynebacterium glutamicum) starting from one single mother cell to densely packed cultures is demonstrated. Applying the picolitre bioreactor, 1.5-fold increased growth rates of C. glutamicum wild type cells were observed compared to typical 1 litre labscale batch cultivation. Moreover, the device was used to analyse and quantify the morphological changes of an industrially relevant L-lysine producer C. glutamicum after artificially inducing starvation conditions. Instead of a one week lab-scale experiment, only 1 h was sufficient to reveal the same information. Furthermore, time lapse microscopy during 24 h picolitre cultivation of an arginine producing strain containing a genetically encoded fluorescence sensor disclosed time dependent single cell productivity and growth, which was not possible with conventional methods.

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Section: Introductionmentioning

confidence: 99%

A disposable picolitre bioreactor for cultivation and investigation of industrially relevant bacteria on the single cell level

et al. 2012

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“…Cells are immobilized in certain regions of the chip after being separated from the flow by different mechanical barriers or obstacles. 32,33 The obstacle or barrier dimensions are similar to the sizes of the cells to be captured. There have been a few ways to trap cells by hydrodynamic trapping: ͑i͒ front-trapping, ͑ii͒ side-trapping, ͑iii͒ gravity trapping, and ͑iv͒ microvortex trapping.…”

Section: Hydrodynamic Trappingmentioning

confidence: 99%

“…The continuous exposure to the force ͑resulting in mechanical movement͒ might lead to a slight increase in temperature, which might cause unexpected physiological damage especially to sensitive cell or tissue types. 32,98 …”

Section: Magnetophoretic Trappingmentioning

confidence: 99%

“…28 The hydrogel is often immobilized with favorable cell-binding motifs that resemble those on the natural ECM in in vivo environments that interact with cells to regulate the cellular functions such as proliferation or differentiation. 32 Various natural and synthetic hydrogels; for instance, calcium alginate 155 and gelatin, 156 have been used in microfluidic systems for cell encapsulation. Recently, cell laden agarose microfluidic hydrogels have been fabricated using a soft lithographic approach.…”

Section: Gel-based System For Chemical Trapping Of Cellsmentioning

confidence: 99%

“…155 Cell immobilization in a microfluidic system is becoming increasingly important as a way of creating artificial tissues. 32 It can also be used for fundamental cell studies for understanding cell-cell interactions and cell responses to soluble stimuli.…”

Section: Engineering Microtissue Constructs For Applicationsmentioning

confidence: 99%

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Exploitation of physical and chemical constraints for three-dimensional microtissue construction in microfluidics

Choudhury

Iliescu

et al. 2011

Biomicrofluidics

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There are a plethora of approaches to construct microtissues as building blocks for the repair and regeneration of larger and complex tissues. Here we focus on various physical and chemical trapping methods for engineering three-dimensional microtissue constructs in microfluidic systems that recapitulate the in vivo tissue microstructures and functions. Advances in these in vitro tissue models have enabled various applications, including drug screening, disease or injury models, and cellbased biosensors. The future would see strides toward the mesoscale control of even finer tissue microstructures and the scaling of various designs for high throughput applications. These tools and knowledge will establish the foundation for precision engineering of complex tissues of the internal organs for biomedical applications.

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