Cell Transformation and the Genesis of Cancer

Bendich, Adrianne; Borenfreund, Ellen; Honda, Yuji; Steinglass, Mildred

doi:10.1080/00039896.1969.10666824

Cited by 8 publications

(3 citation statements)

References 28 publications

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“…The ability of cells to synthesize specific antigens is certainly under gene control. Arguments that oncogenesis is also an example of altered genetic expression have been proposed (5,7,(33)(34)(35), and the results of the present study support them. What is still ambiguous is the nature of the DNA responsible for the heritable effects obtained, and the biochemical mechanism involved.…”

Section: Ch Cells Enmeshed With Dna-deae-d Fibers Still Incorporated supporting

confidence: 87%

“…3 a, b), but only when the cells were in intimate contact. Analogous passage of DNA from mouse leukemia L-5178 to Syrian hamster embryo cells (7), and from a mouse leukemia line to mouse L cells (16) has been described in instances when these cells were cultivated together. Although the possible biological consequences of this kind of cell-to-cell interaction were not elucidated, the results below reveal the emergence of a new type of cell when EA and CH cells are grown together for extended periods.…”

Section: Interactions Between Ehrlich a Scites Tumor And Chinese Hamsmentioning

confidence: 99%

“…The donors were mouse Ehrlich ascites tumor cells and the recipients were nonmalignant Chinese hamster cells. A preliminary account of the background and the results of our earlier experiments has appeared (7).…”

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confidence: 99%

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Studies of Dna-Induced Heritable Alteration of Mammalian Cells

Borenfreund

Honda

Steinglass

et al. 1970

The Journal of Experimental Medicine

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The chemical nature of genetic determinants was eludicated in 1944 by Avery, MacLeod, and McCarty in their classic finding of DNA-mediated transformation of pneumococcal types (1). This provided knowledge of the kinds of biochemical phenomena which are directly controlled by DNA, in bacteria. That DNA is involved in similar functions in somatic mammalian cells is not in dispute; rigorous experimental evidence however has been difficult to obtain (2). Heritable acquisition of genetic activity has resulted from the "infection" of mammalian cells by DNA isolated from oncogenic viruses (3-5), but convincing examples of transformation by mammalian DNA have beenvery rare indeed. Perhaps the most persuasive instance of such change is the recent report (6) that the implantation of amelanotic cells, after incubation with DNA isolated from melanin-producing embryonic cells, into white mice, induced the formation of melanin-producing nodules; unfortunately, controls with DNA from white mice or other sources were not included.The present studies are concerned with heritable acquisition of cellular genetic properties by the exposure of cells to genetically distinct cells or to D N A isolated from them. The properties chosen were the ability to synthesize mouse gene products (antigens), and the expression of oncogenic potential. The donors were mouse Ehrlich ascites tumor cells and the recipients were nonmalignant Chinese hamster cells. A preliminary account of the background and the results of our earlier experiments has appeared (7). Materials and MethodsCells and Reagents.---Chinese hamster (CH) 1 cells were used preferentially because their easily recognizable chromosomes (11 = 22) greatly simplify study of their karyotypes (8, 9).

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Section: Ch Cells Enmeshed With Dna-deae-d Fibers Still Incorporated supporting

confidence: 87%

Section: Interactions Between Ehrlich a Scites Tumor And Chinese Hamsmentioning

confidence: 99%